1887

Abstract

SUMMARY: A strain of , grown in a medium containing a suboptimal concentration (0.4 x 10- ) of biotin, was shown to contain less deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and protein but, during the early stages of growth, increased concentrations of acid-soluble ultraviolet (u.v.)-absorbing substances, as compared with the same organism grown in the presence of an optimal concentration (8.0 x 10 ) of biotin. The concentration of acid-soluble u.v.-absorbing substances in the biotin-deficient yeast was higher, irrespective of the nature of the extracting acid (0.2N-perehloric acid, 5% (w/v) and 10% (w/v) trichloroacetic acid, or 5% (v/v) -butanol in M/15 KHPO). Raising the temperature of extraction from 3° to 21° or 30° had little or no effect on the amounts of these u.v.-absorbing substances extracted. Analyses of the nucleotides and nucleo-bases in the yeast RNA showed these to have a ratio of purine: pyrimidine bases of 1.00–1.15, with the exception of the RNA from 5-day cultures of biotin-deficient yeast which had a slightly but consistently higher ratio. The significance of these results is discussed in relation to the metabolic function of biotin.

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/content/journal/micro/10.1099/00221287-24-1-69
1961-01-01
2020-01-17
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