1887

Abstract

SUMMARY: Washed suspensions of var. were found to contain 10 % by weight of carbohydrate. The only sugars detected in hydrolysates were galactose and ribose. A galactan was obtained by extraction with warm aqueous phenol. The galactan was separated from ribonucleic acid by ultracentrifugation or by treatment with an ion-exchange resin. The product was rapidly hydrolysed by acid, releasing galactose and . 4 % of CHCl- soluble lipid. It formed precipitates with specific antiserum and could be used to sensitize sheep erythrocytes to agglutination by the antiserum. It was not pyrogenic for rabbits and had little in common with the lipopolysaccharides of typical Gram-negative bacteria.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-23-2-357
1960-10-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/23/2/mic-23-2-357.html?itemId=/content/journal/micro/10.1099/00221287-23-2-357&mimeType=html&fmt=ahah

References

  1. Andrews P., Hough L., Jones J. K. N. 1954; The galactan of Strychnos nux-vomica seeds. J. chem. Soc 806:
    [Google Scholar]
  2. Ballance P. E., Crombie W. M. 1958; Paper chromatography of saturated and unsaturated fatty acids. Biochem. J 69:632
    [Google Scholar]
  3. Barker S. A., Stephens R. 1954; Infra-red spectra of carbohydrates. Part IV. Characterisation of furanose derivatives. J. chem. Soc p. 4550
    [Google Scholar]
  4. Barker S. A., Bourne E. J., Stacey M., Whiefen D. H. 1954; Infra-red spectra of carbohydrates. Part I. Some derivatives of d-glucopyranose. J. chem. Soc 171:
    [Google Scholar]
  5. Bartlett G. R. 1959; Phosphorus assay in column chromatography. J. biol. Chem 234:466
    [Google Scholar]
  6. Buchanan J. G., Dekker C. A., Long A. G. 1950; The detection of glycosides and non-reducing carbohydrate derivatives in paper partition chromatography. J. chem. Soc p. 3162
    [Google Scholar]
  7. Buttery S. 1959; Detection of antigens as specific precipitates on paper electrophoresis strips. Nature; Lond.: 183686
    [Google Scholar]
  8. Chen P. S. Jr. Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. Anal. Chem 28:1756
    [Google Scholar]
  9. Conway E. J. 1947 Microdiffusion Analysis and Volumetric Error Revised ed. p. 132 London: Crosby Lockwood;
    [Google Scholar]
  10. Cottew G. S. 1960; Indirect haemagglutination and haemagglutination inhibition with Mycoplasma mycoides. . Aust. vet. J 36:54
    [Google Scholar]
  11. Cummins C. S., Harris H. 1956; The chemical composition of the cell wall in some Gram-positive bacteria and its possible value as a taxonomic character. J. gen. Microbiol 14:583
    [Google Scholar]
  12. Dedonder R. 1952; Sugars and related compounds. Bull. Soc. chim. Fr p. 874
    [Google Scholar]
  13. Dische Z. 1947; A new specific color reaction of hexuronic acids. J. biol. Chem 167:189
    [Google Scholar]
  14. Dische Z. 1953; Qualitative and quantitative colorimetric determination of heptoses. J. biol. Chem 204:983
    [Google Scholar]
  15. Dische Z. 1955; Color reactions of nucleic acid components. In The Nucleic Acids Chargaff E., Davidson J. N. I p. 285 New York: Academic Press Inc.;
    [Google Scholar]
  16. Entenman C. 1957; Preparation and determination of higher fatty acids. In Methods in Enzymology Colowick S. P., Kaplan N. O. Ed. III p. 317 New York: Academic Press Inc;
    [Google Scholar]
  17. Gottschalk A., Ada G. L. 1956; The separation and quantitative determination of the component sugars of mucoproteins. Biochem. J 62:681
    [Google Scholar]
  18. Hanahan D. J., Olley J. N. 1958; Chemical nature of monophosphoinositides. J. biol. Chem 231:813
    [Google Scholar]
  19. Haworth W. N., Raistrick H., Stacey M. 1935; The molecular structure of vari- anose produced from glucose by Penicillium varians G. Smith. Biochem. J 29:2668
    [Google Scholar]
  20. Haworth W. N., Raistrick H., Stacey M. 1937; The molecular structure of galacto-carolose produced from glucose by Penicillium charlesii G. Smith. Biochem. J 31:640
    [Google Scholar]
  21. Heyrovsky A. 1956; A new detection method for paper chromatography of sugars. Biochem. biophys. Acta 21:180
    [Google Scholar]
  22. Hough L., Jones J. K. N., Wadman W. H. 1949; The separation of the sugars and their methylated derivatives on columns of powdered cellulose. J. chem. Soc p. 2511
    [Google Scholar]
  23. Kandler O., Zehender C. 1957; über das Vorkommen von α, ε-Diaminopimelin- säure bei verschiedenen l-Phasentypen von Proteus vulgaris und bei den pleuropneu- monie-ähnlichen Organismen. Z. Naturf 12b:725
    [Google Scholar]
  24. Kurotchkin T. J. 1937; Specific carbohydrate from Asterococcus mycoides for serological tests of bovine pleuropneumonia. Proc. Soc. exp. Biol., N.Y 37:21
    [Google Scholar]
  25. Micheel F., Suckfüll F. 1933; Die Hydrolyse des α-Methyl-galakto-septanosids. Ber. dtsch. chem. Ges 66:1957
    [Google Scholar]
  26. Militzer W. E. 1946; The orcinol reagent. Arch. Biochem 9:85
    [Google Scholar]
  27. Mitchell P. 1949; A new technique for stirred aerated culture. Nature; Lond.: 164846
    [Google Scholar]
  28. Nash T. 1953; The colorimetric estimation of formaldehyde by means of the Hantzsh reaction. Biochem. J 55:416
    [Google Scholar]
  29. Nowotny A., Lüderitz O., Westphal O. 1958; Rudfilter-Chromatographie lang- kettiger Fettsauren bei der Analyse bakterieller Lipopolysaccharide. Biochem. Z 330:47
    [Google Scholar]
  30. O’dea J. F. 1953; Production of formaldehyde during the oxidation of carbohydrates with periodate. Chem. Ind 1338:
    [Google Scholar]
  31. O’dea J. F., Gibbons R. A. 1953; The estimation of small amounts of formaldehyde liberated during the oxidation of carbohydrates and other substances with periodate. Biochem. J 55:580
    [Google Scholar]
  32. O’Neill J. F. 1955; 3,6-anhydro-d-galactose as a constituent of k-carrageenin. J. Amer. chem. Soc 77:2837
    [Google Scholar]
  33. Plackett P. 1959; On the probable absence of mucocomplex from Mycoplasma mycoides. . Biochem. biophys. Acta 35:260
    [Google Scholar]
  34. Rodwell A. W., Rodwell E. S. 1954; The breakdown of carbohydrates by Asterococcus mycoides, the organism of bovine pleuropneumonia. Aust. J. biol. Sci 7:18
    [Google Scholar]
  35. Somogyi M. 1952; Notes on sugar determination. J. biol. Chem 195:19
    [Google Scholar]
  36. Stadtman T. C. 1957; Prepiaration and assay of cholesterol and ergosterol. In Methods in Enzymology Colowick S. P., Kaplan N. O. Ed. III p. 392 New York: Academic Press Inc;
    [Google Scholar]
  37. Stern I., Shapiro B. 1953; A rapid and simple method for the determination of esteri-fied fatty acids and for total fatty acids in blood. J. clin. Path 6:158
    [Google Scholar]
  38. Trevelyan W. E., Harrison J. S. 1952; Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem. J 50:298
    [Google Scholar]
  39. Turner A. W., Campbell A. D., Dick A. T. 1935; Recent work on pleuropneumonia contagiosa bourn in North Queensland. Aust. vet. J 11:63
    [Google Scholar]
  40. Vasseur E. 1948; A spectrophotometric study on the orcinol reaction with carbohydrates. Acta chem. scand 2:693
    [Google Scholar]
  41. Westphal O., Lüderitz O., Bister F. 1952; über die Extraktion von Bakterien mit Phenol/Wasser. Z. Naturf 7b:148
    [Google Scholar]
  42. Westphal O., Lüderitz O., Eichenberger E., Keiderling W. 1952; Über bakterielle Reizstoffe I. Mitt.: Reindarstellung eines Polysaccharid-Pyrogens aus Bacterium coli. . Z. Naturf 7b:536
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-23-2-357
Loading
/content/journal/micro/10.1099/00221287-23-2-357
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error