1887

Abstract

SUMMARY: Oligotrich protozoa from the sheep rumen, principally , were maintained in culture and dividing every 2 days at a density of 15,000–30,000 protozoa/ml. for 18 months on a medium of rice starch, dried grass, 10% (v/v) rumen fluid (fresh or autoclaved) and 50 g. chloramphenicol/ml. The effect on these organisms of varying each of these factors in turn and the use of different growth conditions is described. The protozoa have also been grown from an inoculum of 100’900/ml. to a density of over 50,000/ml. in 10 days.

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/content/journal/micro/10.1099/00221287-22-2-555
1960-04-01
2021-07-27
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References

  1. Becker E. R., Schultz J. A., Emmerson M. A. 1929; Experiments on the physiological relationships between the stomach infusoria of ruminants and their hosts, with a bibliography. Iowa St. Coll. J. Sci 4:215
    [Google Scholar]
  2. Bhatia B. L. 1936 The Fauna of British India Protozoa-Ciliophora London: Taylor and Francis;
    [Google Scholar]
  3. Coleman G. S. 1958; Maintence of oligotrich protozoa fromthe sheep rumen in vitro . Nature; Lond: 1821104
    [Google Scholar]
  4. Gutierrez J., Davis R. E. 1959; Bacterial ingestion by the rumen ciliates Entodinium and Diplodinium . J. Protozool 6:222
    [Google Scholar]
  5. Hungate R. E. 1942; The culture of Eudiplodinium neglectum with experiments on the digestion of cellulose. Biol. Bull Woods Hole 83:303
    [Google Scholar]
  6. Hungate R. E. 1943; Further experiments on cellulose digestion by the protozoa in the rumen of cattle. Biol. Bull Woods Hole 84:157
    [Google Scholar]
  7. Kandatsu M., Takahashi N. 1955a; Studies on reticulo-rumen digestion Part 2 on the articlecultureof some Entodinia I. J. Agric. Chem. Soc Japan: 29833
    [Google Scholar]
  8. Kandatsu M., Takahashi N. 1955b; Studies on reticulo-rumen digestion Part 2 on the articlecultureof some Entodinia . J. Agric. Chem. Soc Japan: 29915
    [Google Scholar]
  9. Kandatsu M., Takahashi N. 1956; Studies on reticulo-rumen digestion. Part 4. On the artificial culture of some Entodinia. III. J. Agric. Chem. Soc 30:96
    [Google Scholar]
  10. McPherson M. J. 1953; Isolation and identification of amylolytic Streptococci from the rumen of the sheep. J. Path. Bact 66:95
    [Google Scholar]
  11. Margolin S. 1930; Methods for the cultivation of cattle ciliates. Biol. Bull Woods Hole 59:301
    [Google Scholar]
  12. Stone H. W., Beeson C. 1936; Preparation and storage of standard chromous sulphate solution. Industr. Engng Chem. (Anal.) 8:188
    [Google Scholar]
  13. Sugden B. 1953; The cultivation and metabolism of oligotrich protozoa from the sheep’s rumen. J. gen. Microbiol 9:44
    [Google Scholar]
  14. Westphal A. 1934; Studien über Ophryoscoleciden in der Kultur. Z Parasitenk 7:71
    [Google Scholar]
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