1887

Abstract

SUMMARY: The formation of acetoin was studied with 44 strains of the genus when growing on a medium containing -lactate as the main carbon source. Most of the strains produced only limited amounts of acetoin. However, strains of the species and converted most of their substrate into acetoin, up to 74 % of the theoretical amount. The influence of the substrate concentration and of the degree of aeration was studied.

The Voges-Proskauer positive substance was isolated from large-scale fermentations, purified and definitely identified as acetoin. It was present chiefly in the laevo- rotatory form. Young resting cells oxidized ( —)- lactate, ( + )- lactate, sodium pyruvate, acetoin and diacetyl, often nearly to completion. Cell-free extracts synthesized acetoin from pyruvate optimally at about pH 7. More than 99 of the enzyme activity was located in the soluble enzyme fraction. Thiamin pyrophosphate stimulated CO and acetoin production from pyruvate. Evidence is presented that acetoin may arise both from the acetaldehyde and from the a-acetolactate pathway.

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1959-10-01
2024-04-26
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References

  1. Berl S., Bueding E. 1951; Metabolism of acetylmethylcarbinol in filariae. J. Mol. Chem 191:401
    [Google Scholar]
  2. De Ley J. 1958; Studies on the metabolism of Acetobacter peroxydans. I. General properties and taxonomic position of the species. Ant. v. Leeuwenhoek J. Microbiol. Serol 24:281
    [Google Scholar]
  3. De Ley J., Schell J. 1959; Studies on the metabolism of Acetobacter peroxydans. II.The enzymic mechanism of lactate metabolism. Biochim. biophys. Acta in the Press
    [Google Scholar]
  4. Dolin M. I., Gunsalus I. C. 1951; Pyruvic acid metabolism. II. An acetoin- forming enzyme system in Streptococcus faecalis . J. Bact 62:199
    [Google Scholar]
  5. Frateur J. 1950; Essaisur la systématique des Acétobacters. Cellule 53:287
    [Google Scholar]
  6. Fulmer E. I., Underkofler L. A., Bantz A. C. 1948; The production of acetylmethylcarbinol by the action of Acetobacter suboxydans upon 2,3-butylene glycol. J. Amer. chem. Soc 65:1425
    [Google Scholar]
  7. Gunsalus I. C., Horecker B. L., Wood W. A. 1955; Pathways of carbohydrate metabolism in microorganisms. Bact. Rev 19:79
    [Google Scholar]
  8. Hermann S., Neuschull P. 1931; Zur Biochemie der Essigbakterien. Zugleichein Vorschlag für ein neue Systematik. Biochem. Z 233:129
    [Google Scholar]
  9. Hermann S., Neuschull P. 1932; Milchsäure- und Brenztraubensäureabbaudurch Essigbakterien. Biochem. Z 246:446
    [Google Scholar]
  10. Juni E. 1952a; Mechanism of formation of acetoin by bacteria. J. biol. Chem 195:715
    [Google Scholar]
  11. Juni E. 1952b; Mechanism of formation of acetoin by yeast and mammalian tissue. J. biol. Chem 195:727
    [Google Scholar]
  12. Juni E., Heym G. A. 1953; A new pathway for the bacterial dissimilation of diacetyl. Bact. Proc p. 81
    [Google Scholar]
  13. Juni E., Heym G. A. 1954; Diphosphothiamine dependent condensation reactions. Fed. Proc 13:238
    [Google Scholar]
  14. Juni E., Heym G. A. 1956; A cyclic pathway for the bacterial dissimilation of 2,3-butanediol, acetylmethylcarbinol and diacetyl. J. Bact 71:425
    [Google Scholar]
  15. Kitasato T. 1928; Aeyloin-Aufbau mittels Enzymen der Essigbakterien. Biochem. Z 195:118
    [Google Scholar]
  16. Kitos P. A., King T. E., Cheldelin V. H. 1956; Carbohydrate metabolic pathways in Acetobacter suboxydans . Fed. Proc 15:943
    [Google Scholar]
  17. Kitos P. A., King T. E., Cheldelin V. H. 1957; Metabolism of fructose-1,6-diphosphate and acetate in Acetobacter suboxydans . J. Bact 74:565
    [Google Scholar]
  18. Kobel M., Hackenthal E. 1941; Enzymatische Kondensationen (Karboligase). In BAMANN, E. & MYRBÄCK, K., Die Methoden der Fermentforschung p. 2206 Leipzig: Thieme;
    [Google Scholar]
  19. Rainbow C., Mitson G. W. 1953; Nutritional requirements of acetic acid bacteria. J. gen. Microbiol 9:371
    [Google Scholar]
  20. Rao M. R. R. 1955; Pyruvate and acetate metabolism in Acetobacter suboxydans and Acetobacter aceti. Thesis Urbana, Ill, U.S.A:
    [Google Scholar]
  21. Singer T. P., Pensky J. 1952; Mechanism of acetoin synthesis by α-carboxylase. Biochim. Biophys. Acta 9:316
    [Google Scholar]
  22. Tanko B., Munk L., Abonyi I. 1940; Über die optische Aktivität der durch biochemische Synthese entstandenen Acetoine. Z. physiol. Chem 264:91
    [Google Scholar]
  23. Westerfeld W. W. 1945; A colorimetric determination of blood acetoin. J. biol. Chem 161:495
    [Google Scholar]
  24. White A. G. C., Krampitz L. O., Werkman C. H. 1946; Method for the direct determination of diacetyl in tissue and bacterial filtrates. Arch. Biochem 9:229
    [Google Scholar]
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