1887

Abstract

SUMMARY: Amino acid-requiring mutants of were examined for their ability to regenerate flagella when incubated with a source of nitrogen and of energy, but in the absence of the amino acid required for growth. The mutants fell into two major groups and one intermediate group. The major groups were: () mutants requiring for growth an amino acid (such as leucine or tyrosine) present in flagellin and unable to regenerate flagella in the absence of this amino acid, () mutants requiring for growth an amino acid (such as tryptophan or cysteine) not present in flagellin and able to regenerate flagella in the absence of this amino acid. The intermediate group () contained mutants requiring for growth an amino acid (such as histidine or methionine) present in small amount in flagellin and able to regenerate flagella in the absence of this amino acid. With all the mutants tested there was no significant net synthesis of RNA or protein in the absence of the amino acid required for growth, but DNA synthesis did occur.

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1959-08-01
2022-01-23
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References

  1. Burton K. 1956; A study of the conditions and mechanism of the diphenyl-amine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  2. Cohn M. 1957; Contributions of the studies on the β-galactosidase of Escherichia coli to our understanding of enzyme synthesis. Bact. Rev 21:140
    [Google Scholar]
  3. Demerec M., Hartman Z., Hartman P. E., Yura T., Demerec Z. E., Gots J. S., Ozeki H., Glover S. W. 1956; Genetic studies with bacteria. Publ. Carneg. Instn612
    [Google Scholar]
  4. Fox E. N., Krampitz L. O. 1956; Studies on the biosynthesis of the M protein of group A haemolytic streptococci. J. Bact 71:454
    [Google Scholar]
  5. Lark K. G., MaalØe O. 1956; Nucleic acid and the division cycle of Salmonella typhimurium . Biochim. Biophys.Acta 21:448
    [Google Scholar]
  6. Leifson E. 1951; Staining, shape and arrangement of bacterial flagella. J. Bact 62:377
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein estimation with the folin phenol reagent. J. biol. Chem 192:265
    [Google Scholar]
  8. Mandelstam J. 1958; Turnover of protein in growing and non-growing populations of Escherichia coli . Biochem. J 69:110
    [Google Scholar]
  9. Morse M. L., Carter C. E. 1949; The synthesis of nucleic acids in cultures of Escherichia coli strains B and B/R. J. Bact 58:317
    [Google Scholar]
  10. Ogur M., Rosen G. 1950; The nucleic acids of plant tissues. 1. The extraction and estimation of RNA and DNA. Arch. Biochem. Biophys 25:262
    [Google Scholar]
  11. Pardee A. B., Prestridge L. S. 1956; The dependence of nucleic acid synthesis on the presence of amino-acids in Escherichia coli . J. Bact 71:677
    [Google Scholar]
  12. Pollock M. R. 1946; Adaptation of ‘nitratase’ in washed suspensions of bacteria. Brit. J. exp. Path 27:419
    [Google Scholar]
  13. Quadling C. 1958; The unilinear transmission of motility and its material basis in Salmonella . J. gen. Microbiol 18:237
    [Google Scholar]
  14. Rinker J. N., Kobayashi T., Koffler H. 1957; Isolation and chemical properties of flagellar proteins. Fed. Proc 16:238
    [Google Scholar]
  15. Roberts R. B., Cowie D. B., Abelson P. H., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia coli . Publ. Carneg. Instn607
    [Google Scholar]
  16. Stocker B. A. D. 1957; Methods of removing flagella from live bacteria; effects on motility. J. Path. Bact 73:314
    [Google Scholar]
  17. Weibull C. 1953; The free amino-groups of the Proteus flagellar protein. Quantitative determinations of the DNP amino-acids using paper chromatography. Acta Chem. Scand 7:233
    [Google Scholar]
  18. Zinder N. D., Lederberg J. 1952; Genetic exchange in Salmonella . J. Bact 64:679
    [Google Scholar]
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