SUMMARY: Protoplasts of 569 and 569/H were shown to be as competent as intact cells in the induced and constitutive formation of penicillinase, as well as in other biochemical activities. It was demonstrated that the cell-bound penicillinase was localized at or near the cell surface. The β-penicillinase fraction was released by digestion of the cell wall and by washing of the protoplast membrane. However, the γ-penicillinase was tightly bound to the cytoplasmic membrane. It was obtained in a soluble form by treating the membrane with sonic vibration or with fat solvents.


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