SUMMARY: B166 synthesizes as much vitamin B when growing on serine or glycine plus glycolaldehyde as does its parent ( B) on unsupplemented medium. Suspensions of strain B166 harvested after growth on serine+ glycine plus glycolaldehyde, and incubated with glucose and NH , only synthesize vitamin B when glycolaldehyde is added; serine + glycine increases the synthesis. Strain 22–99 produces more vitamin B when grown on glycolaldehyde plus serine + glycine than when it is grown on the amino acids alone, while, depending upon the conditions of growth, vitamin B synthesis by suspensions is absolutely or partially dependent on serine+ glycine plus glycolaldehyde. However, serine + glycine plus glycolaldehyde have little effect on the biosynthesis of vitamin B by wild-type during growth, or in washed suspensions.


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