Above a value of one, repeatedly doubling the multiplicity of infection of chick embryo cells by vesicular stomatitis virus progressively shortened the latent period by about 0·6 hr.; this phenomenon is referred to as ‘shortened latency’. Varying the multiplicity above unity with dilute-passage stocks did not interfere with rate of infective virus release, number of cells infected, or final yield, i.e. there was no ‘von Magnus’ effect or other obvious interference phenomena. The doubling time for virus release was also about 0·6 hr. This suggested that virus may have been growing as a simple intracellular pool equally accessible to all adsorbing virus, and that 1 particle was released when the pool reached a certain size (perhaps 20–200 units) irrespective of inocula. However, other explanations are possible, and of those allowing experimental test, earlier initial adsorption of virus, multiplicity reactivation amongst a partly inactivated population, more rapid elution of attached virus or more rapid release of accumulated internal virus could not account for shortened latency.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error