1887

Abstract

Summary: Investigations on the size of inhibition zones in agar tube cultures of (Mayo) due to diffusion of streptomycin have confirmed the formula previously suggested. No significant deviation from expectations has been found over a temperature range of 27–40°, nor with variations of inoculum size from 2 × 10 to 5 × 10 organisms/ml. The theoretical basis of the formula has been further elucidated and the meaning of and clarified. These constants are determined by the necessity of absorbing a of antibiotic on the organisms to inhibit growth within the zone. To maintain this absorption a minimum inhibitory concentration must remain in the surrounding medium.

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/content/journal/micro/10.1099/00221287-18-3-670
1958-06-01
2021-10-19
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References

  1. Berkman S., Henry R.J., Housewright R.D., Henry J. 1948; Streptomycin. IV. Absorption of streptomycin by susceptible and resistant bacteria. Proc. Soc. exp. Biol., N.Y 68:65
    [Google Scholar]
  2. Burroughs, Wellcome and Co., London. Opacity tables A and B.
  3. Clarke A.J. 1933 The Mode of Action of Drugs on Cells p. 44 London: Edward Arnold and Co.;
    [Google Scholar]
  4. Cooper K.E. 1952; Note on the theory of inhibition zones. J. gen. Microbiol 7:13
    [Google Scholar]
  5. Cooper K.E. 1955; Theory of antibiotic inhibition zones in agar media. Nature; Lond.: 176510
    [Google Scholar]
  6. Cooper K.E., Gillespie W.A. 1952; The influence of temperature on Streptomycin inhibition zones in agar cultures. J.gen. Microbiol 7:1
    [Google Scholar]
  7. Cooper K.E., Linton A.H. 1952; The importance of the temperature during the early hours of incubation of agar plates in assays. J. gen. Microbiol 7:8
    [Google Scholar]
  8. Cooper K.E., Woodman D. 1946; The diffusion of antiseptics through agar gels, with special reference to the agar cup assay method of estimating the activity of penicillin. J. Path. Bact 58:75
    [Google Scholar]
  9. Eagle H., Levy M., Fleischman R. 1955; The binding of Penicillin in relation to its cytotoxic action. IV. The amounts bound by bacteria at ineffective, growth-inhibitory, bactericidal and maximally effective concentrations. J. Bact 69:167
    [Google Scholar]
  10. Gale E.F. 1943; Factors influencing the enzymic activities of Bacteria. Bad. Rev 7:139
    [Google Scholar]
  11. Gillissen G. 1954; Der Agar-Diffusionstest (Lochtest) als Routineverfahren. Zbl. Bakt.(1 Org.) 161:482
    [Google Scholar]
  12. Herdan G. 1949; Assay of antiseptics using a Gaussian co-ordinate system. Research 2:590
    [Google Scholar]
  13. Hershey A.D. 1938; Factors limiting bacterial growth. II. Growth without lag in Bacterium coli cultures. Proc. Soc. exp. Biol., N.Y 38:127
    [Google Scholar]
  14. Hershey A.D. 1939; Factors limiting bacterial growth. IV. The age of the parent culture and the rate of growth of transplants of E. coli. . J. Bact 37:285
    [Google Scholar]
  15. Hinshelwood C.N. 1946 The Chemical Kinetics of the Bacterial Cell p. 40 Oxford University Press;
    [Google Scholar]
  16. Humphrey J.H., Lightbown J.W. 1952; A general theory for plate assay of antibiotics with some practical applications. J. gen. Microbiol 7:129
    [Google Scholar]
  17. Ingram G.I.C., Foxell A.W.H., Armitage P. 1953; The nature of the dose-response curve in penicillin plate assay. Antibiol. & Chemother 3:1247
    [Google Scholar]
  18. Johnson F.H., Lewin I. 1946; The action of quinine on dehydrogenases of E. coli. The disinfection of E. coli in relation to temperature, hydrostatic pressure and quinine. J. cell. comp. Physiol 28:2347
    [Google Scholar]
  19. Kaye C.W.C., Laby T.H. 1944 Physical and Chemical Constants and some Mathematical Functions, 9th ed.. p. 39 London: Longmans, Green and Co.;
    [Google Scholar]
  20. Linton A.H. 1949 Action of antibiotics on inhibition zones Thesis M.Sc. Bristol University;
    [Google Scholar]
  21. Linton A.H. 1954 Studies in the viability of bacterial populations Thesis Ph.D. Bristol University;
    [Google Scholar]
  22. Linton A.H. 1955; The relationship between growth rate of different bacteria and the formation of antibiotic inhibition zones. J. gen. Microbiol 13:viii
    [Google Scholar]
  23. Masuyama G. 1948; An assay method and a basic formula by use of one-dimensional diffusion. Kagaku 17:158
    [Google Scholar]
  24. Mayr-Harting A. 1947; The growth of bacterial colonies and their viable population. J. Hyg., Camb 45:19
    [Google Scholar]
  25. Mcquillen K. 1951; The bacterial surface. IV. Effect of streptomycin on the electrophoretic mobility of Escherichia coli and Staphylococcus aureus. . Biochem. biophys. Acta 7:54
    [Google Scholar]
  26. Miles A.A., Misra S.S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 38:732
    [Google Scholar]
  27. Mitchison D.A., Spicer C.C. 1949; A method of estimating streptomycin in serum and other body fluids by diffusion through Agar enclosed in glass tubes. J. gen. Microbiol 3:184
    [Google Scholar]
  28. Miyamura S. 1953; Determination of the sensitivity of micro-organisms to antibiotics by the agar plate diffusion method. Antibiot. & Chemother 3:903
    [Google Scholar]
  29. Schlesinger F.G. 1954; Sensitivity tests by means of diffusion methods and their clinical interpretation. Acta med. scand 148:357
    [Google Scholar]
  30. Schonfeld J.K. 1953 The investigation of bacterial sensitivity in regard to antibiotics Thesis Leiden University;
    [Google Scholar]
  31. Sykes G., Lewis D.G., Goshawk B. 1951; Preliminary observations on the effect of some sugars on the assay of penicillin. Proc. Soc. appl. Bact 14:40
    [Google Scholar]
  32. Vesterdal J. 1947; Studies on the inhibition zones observed in the agar cup method of penicillin assay. Acta path, microbiol. Scand 24:272
    [Google Scholar]
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