1887

Abstract

SUMMARY: A turbidimetric assay is described for antibiotic substances produced by NCIB 8132, and NCIB 4940, based on the amount of inhibition of growth of a celluloseless mutant. Yield of antibiotic from each organism was independent of the source of nitrogen in the medium, but varied markedly with the carbon source and was highest when the organisms were grown for 10 days at 30° in a medium containing glucose (9 %, w/v), Difco yeast- extract (0·5 %, w/v) and peptone (Evans, 3 % w/v), in distilled water. Under these conditions growth of the assay organism was inhibited 50 % by filtrate diluted 1/44. The antibiotics were extremely alkali-labile being destroyed at 18° in 2 min. at pH 10. Activity of the filtrate from organism NCIB 8132 was not affected significantly by treatment with calcium carbonate, charcoal, cation- or anion-exchange resins, but was decreased by extraction at pH 3·0 with w-butanol, chloroform or methyl ethyl ketone. Activity of filtrate of organism NCIB 4940 was decreased by treatment with charcoal, cation- or anion-exchange resins and by extraction at pH 3·0 with benzene, -butanol, chloroform, diethyl ether, ethyl acetate or methyl ethyl ketone.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-18-2-369
1958-04-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/18/2/mic-18-2-369.html?itemId=/content/journal/micro/10.1099/00221287-18-2-369&mimeType=html&fmt=ahah

References

  1. Brownlee K. A. 1949 Industrial Experimentation, 4th ed. London: Her Majesty’s Stationery Office;
    [Google Scholar]
  2. Clayton J. C., Hems B. A., Robinson F. A., Andrews R. D., Hunwicke R. F. 1944; Preparation of penicillin.Improved method of isolation. Biochem. J 38:452
    [Google Scholar]
  3. Kulka D., Walker T. K. 1954; The ketogenic activities of Acetobacterspecies in a glucose medium. Arch. Biochem. Biophys 50:169
    [Google Scholar]
  4. Steel R., Walker T. K. 1957a; Celluloseless mutants of certain Acetobacter species. J. gen. Microbiol 17:12
    [Google Scholar]
  5. Steel R., Walker T. K. 1957b; A highly specific growth-inhibitory factor produced by certain Acetobacter species. Nature; Lond: 180 201
    [Google Scholar]
  6. Tosic J., Walker T. K. 1946; Procedure for the characterisation of the acetic acid bacteria. Chem. & Ind. (Rev.) 65:104
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-18-2-369
Loading
/content/journal/micro/10.1099/00221287-18-2-369
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error