1887

Abstract

SUMMARY: The nucleic acids were isolated from by extraction of mechanically disintegrated organisms with 0·14 -sodium chloride at pH 7·5. Upon subsequent isolation, about 80 % of the deoxyribonucleic acid (DNA) originally contained in the organisms was obtained, but only about 50% of the ribonucleic acid (RNA). The loss of RNA was mainly due to enzymic degradation. By extraction of with 1 % sodium cholate at 60°, enzymic degradation was virtually eliminated and a good yield of both DNA and RNA obtained. They were relatively undegraded and both showed evidence of the presence of hydrogen bonds. The amounts of the purine and pyrimidine bases in both types of nucleic acid were determined. The DNA and RNA from the normal streptomycin-sensitive strain of differed significantly in base composition from the corresponding nucleic acids from a strain which had been trained to grow in 5 % (w/v) streptomycin.

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/content/journal/micro/10.1099/00221287-17-3-586
1957-12-01
2021-10-18
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References

  1. Alexander H. E., Leidy G. 1953; Induction of streptomycin resistance in sensitive Haemophilusinfluenzae by extracts containing deoxyribonucleic acid from resistant H. influenzae. J. exp. Med 97:17
    [Google Scholar]
  2. Avery O. T., Macleod C. M., Mccarty M. 1944; Chemical nature of the substance inducing transformation of pneumococcal types. Induction of transformation by a deoxyribonucleic acid fraction isolated from pneumococcus type III. J. exp. Med 79:137
    [Google Scholar]
  3. Cox R. A., Jones A. S., Marsh G. E., Peacocke A. R. 1956; On hydrogen bonding and branching in a bacterial ribonucleic acid. Biochim. biophys. Acta 21:576
    [Google Scholar]
  4. Cox R. A., Peacocke A. R. 1956; Electrometric titration of the sodium salts of deoxyribonucleic acids. Part IV. Denaturation by heat in aqueous solution. J. chem. Soc p. 2646
    [Google Scholar]
  5. Crestfield A. M., Smith K. C., Allen F. W. 1955; The preparation and characterisation of ribonucleic acids from yeast. J. biol. Chem 216:185
    [Google Scholar]
  6. Crosbie G. W., Smellie R. M. S., Davidson J. N. 1953; Phosphorus compounds in the cell. V. Composition of the cytoplasmic and nuclear ribonucleic acids of the liver cell. Biochem. J 54:287
    [Google Scholar]
  7. Dische Z. 1930; Über einige neue charakteristische Farbreaktionen der Thymo-nucleinsäure und eine Mikromethode zur Bestimmung derselben in tierischen Organen mit Hilfe dieser Reaktionen. Mikrochemie 8:4
    [Google Scholar]
  8. Dorner R. W., Knight C. A. 1953; The preparation and properties of some plant virus nucleic acids. J. biol. Chem 205:959
    [Google Scholar]
  9. Dubois M., Gilles K., Hamilton J. K., Rebers P. A., Smith F. 1951; A colorimetric method for the determination of sugars. Nature; Lond.: 168167
    [Google Scholar]
  10. Dutta S. K., Jones A. S., Stacey M. 1956; The nucleic acids of Sarcina lutea. J. gen. Microbiol 14:160
    [Google Scholar]
  11. Eagle H., Saz A. K. 1955; Antibiotics. Annu. Rev. Microbiol 9:200
    [Google Scholar]
  12. Elson D., Chargaff E. 1955; Evidence of common regularities in the composition of pentosenucleic acid. Biochim. biophys. Acta 17:367
    [Google Scholar]
  13. Elson D., Trent L. W., Chargaff E. 1955; The nucleotide composition of pentosenucleic acids in different cellular fractions. Biochim. biophys. Acta 17:362
    [Google Scholar]
  14. Foster A. B. 1952; A simplified apparatus for filter paper ionophoresis. Chem. & Ind p. 1050
    [Google Scholar]
  15. Foster A. B., Jones A. S., Rizvi S. B. H. 1956; Ionophoresis of nucleic acid components. Chem. & Ind p. 914
    [Google Scholar]
  16. Grinnan E. L., Mosher W. A. 1951; Highly polymerised ribonucleic acid.Preparation from liver and depolymerisation. J. biol. Chem 191:719
    [Google Scholar]
  17. Henry H., Stacey M. 1946; Histochemistry of the Gram staining reaction for micro-organisms. Proc. Roy. Soc. B 133:391
    [Google Scholar]
  18. Hunter G. D., Butler J. A. V. 1956; Stimulation by ribonucleic acid of induced β-galactosidase formation in Bacillus megaterium. Biochim. biophys. Acta 20:406
    [Google Scholar]
  19. Jones A. S., Marsh G. E. 1954; The deproteinisation of nucleoproteins. Biochim. biophys. Acta 14:559
    [Google Scholar]
  20. Kay E. R. M., Dounce A. L. 1953; The preparation of sodium ribonucleate with the use of sodium dodecyl sulphate. J. Amer. chem. Soc 75:4041
    [Google Scholar]
  21. Kirby K. S. 1956; A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem. J 64:405
    [Google Scholar]
  22. Laland S. G., Lee W. A., Overend W. G., Peacocke A. R. 1954; The ultraviolet absorption of some degraded deoxyribonucleic acids. Biochim. biophys. Acta 14:356
    [Google Scholar]
  23. Lombard A., Chargaff E. 1956; The pentosenucleic acid of Azotobacter vinelandii. Biochim.biophys. Acta 20:585
    [Google Scholar]
  24. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  25. Mitchell P., Moyle J. 1954; The Gram reaction and cell composition: Nucleic acids and other phosphate fractions. J. gen. Microbiol 10:533
    [Google Scholar]
  26. Schmidt G., Thannhauser S. J. 1945; A method for the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues. J. biol. Chem 161:83
    [Google Scholar]
  27. Schneider W. C. 1945; Phosphorus compounds in animal tissues. I. Extraction and estimation of deoxypentosenucleic acid and pentosenucleic acid. J. biol. Chem 161:293
    [Google Scholar]
  28. Wyatt G. R. 1951; The purine and pyrimidine composition of deoxypentosenucleic acids. Biochem. J 48:584
    [Google Scholar]
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