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Volume 17, Issue 3

The Metabolism of Inorganic Polyphosphate in Mycobacteria Free

Abstract

SUMMARY: Cell-free extracts of contain an inorganic polyphosphatase and a ‘polyphosphate-AMP-phosphotransferase’. The latter enzyme, which synthesizes adenosine triphosphate from adenosine-5-monophosphate and inorganic polyphosphate, has a pH optimum of about 6·3, and is stimulated by Mg. It does not appear to be attached to cell particles, and a slight purification may be effected by ammonium sulphate precipitation.

With a tracer technique it was shown that cellular inorganic polyphosphate is probably not the sole precursor either of lipid phosphorus or of nucleic acid phosphorus in . The rapid accumulation of insoluble polyphosphate caused in and but not , by a number of organic substances, particularly alcohols, was studied. This accumulation takes place even after inoculation into a fresh nitrogenous medium, when polyphosphate normally decreases.

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© Society for Gerenal Microbiology, 1957
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1957-12-01
2024-04-26
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The Metabolism of Inorganic Polyphosphate in Mycobacteria
Microbiology 17, 573 (1957); https://doi.org/10.1099/00221287-17-3-573
/content/journal/micro/10.1099/00221287-17-3-573
/content/journal/micro/10.1099/00221287-17-3-573
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