1887

Abstract

SUMMARY: Cell-free extracts of contain an inorganic polyphosphatase and a ‘polyphosphate-AMP-phosphotransferase’. The latter enzyme, which synthesizes adenosine triphosphate from adenosine-5-monophosphate and inorganic polyphosphate, has a pH optimum of about 6·3, and is stimulated by Mg. It does not appear to be attached to cell particles, and a slight purification may be effected by ammonium sulphate precipitation.

With a tracer technique it was shown that cellular inorganic polyphosphate is probably not the sole precursor either of lipid phosphorus or of nucleic acid phosphorus in . The rapid accumulation of insoluble polyphosphate caused in and but not , by a number of organic substances, particularly alcohols, was studied. This accumulation takes place even after inoculation into a fresh nitrogenous medium, when polyphosphate normally decreases.

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1957-12-01
2021-08-06
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References

  1. American Trudeau Society 1950; Report on the Laboratory Sub-commmittee of the Committee on Medical Research and Therapy. Amer. Rev. Tuberc 61274
    [Google Scholar]
  2. Cohn W. E., Carter C. E. 1950; The separation of adenosine polyphosphates by ion exchange and paper chromotography. J. Amer. chem. Soc 72:4273
    [Google Scholar]
  3. Davis J. C., Mudd S. 1955; The cytology of a strain of Corynebacterium diphtheriae . J. Bact 69:372
    [Google Scholar]
  4. Duguid J. P., Smith I. W., Wilkinson J. F. 1954; Volutin production in Bacterium aerogenesdue to development of an acid reaction. J. Path. Bact 67:289
    [Google Scholar]
  5. Hoffmann-Ostenhof O., Kenedy J., Keck K., Gabriel O., Schönfellinger H. W. 1954; Ein neues Phosphat-übertragendes Ferment aus Hefe. Biochim. biophys. Acta 14:285
    [Google Scholar]
  6. Hoffmann-Ostenhof O., Klima A., Kenedy J., Keck K. 1955; Zur Kenntnis des Phosphatstoffwechsels der Hefe. I. Quantitative Untersuchungen iiber das Verhalten verschiedener Phospliatfraktionen unter bestimmten Stoffwechselbedingungen. Mh. Chem 86:604
    [Google Scholar]
  7. Juni E., Kamen M. D., Spiegelman S., Wiame J. M. 1947; Physiological heterogeneity of metaphosphate in yeast. Nature; Lond.: 160717
    [Google Scholar]
  8. Juni E., Kamen M. D., Reiner J. M., Spiegelman S. 1948; Turnover and distribution of phosphate compounds in yeast metabolism. Arch. Biochem 18:387
    [Google Scholar]
  9. Katchman B. J., Van Wazer J. R. 1954; The ‘soluble’ and ‘insoluble’ polyphosphates of yeast. Biochim. biophys. Acta 14:445
    [Google Scholar]
  10. Kornberg A., Kornberg S. R., Simms E. S. 1956; Metaphosphate synthesis by an enzyme from Escherichia coli . Biochim. biophys. Acta 20:215
    [Google Scholar]
  11. Lieberman I., Eto W. H. 1956; Inorganic triphosphate synthesis by muscle adenylate kinase. J. biol. Chem 219:307
    [Google Scholar]
  12. Meissner J., Diller W. 1953; Über die Aufnahme von Ortho-und Metaphosphate durch das Mycobacterium tuberculosis . Z. Hyg. InfektKr 137:518
    [Google Scholar]
  13. Meissner J., Kropp F. 1953; Über die Phosphatresorption beim Mycobacterium tuberculosis . Z. Hyg. InfektKr 137:429
    [Google Scholar]
  14. Meissner J., Lemke J. 1955; Über die Aufnahme 32P-markierter kondensierter Phosphate bei BCG- und H 37-Stämmen von Mycobacterium tuberculosis . Z. Hyg. InfektKr 141:249
    [Google Scholar]
  15. Oliver I. T., Peel J. L. 1956; Myokinase activity in microorganisms. Biochim. biophys. Acta 20:390
    [Google Scholar]
  16. Ruska H., Bringmann G., Neckel I., Schuster G. 1952; Über die Entwicklung sowie den morphologischen und zytochemischen Aufbau v. Mycobacterium avium(Chester). Z. wiss. Mikr 60:425
    [Google Scholar]
  17. Sall T., Mudd S., Davis J. C. 1956; Factors conditioning the accumulation and disappearance of metaphosphate in cells of Corynebacterium diphtheriae . Arch. Biochem. Biophys 60:130
    [Google Scholar]
  18. Smith I. W., Wilkinson J. F., Duguid J. P. 1954; Volutin production in Aerobacter aerogenesdue to nutrient imbalance. J. Bact 68:450
    [Google Scholar]
  19. Stich H. 1953; Der Nachweis und das Verhalten von Metaphosphaten in normalen, verdunkelten und Trypaflavin-behandelten Acetabularien . Z. Naturf 8b:36
    [Google Scholar]
  20. Waton N. G. 1956; The effect of organic solvents on mammalian histidine decarboxylase. Biochem. J 64:318
    [Google Scholar]
  21. Wiame J. M. 1949; The occurrence and physiological behaviour of two metaphosphate fractions in yeast. J. biol. Chem 178:919
    [Google Scholar]
  22. Winder F., Denneny J. M. 1954; Metaphosphate in mycobacterial metabolism. Nature; Lond.: 174353
    [Google Scholar]
  23. Winder F., Denneny J. M. 1955; Utilization of metaphosphate for phosphory-lation by cell-free extracts of Mycobacterium smegmatis . Nature; Lond.: 175636
    [Google Scholar]
  24. Winder F. G., Denneny J. M. 1956; Phosphorus metabolism of mycobacteria: determination of phosphorus compounds in some mycobacteria. J. gen. Microbiol 15:1
    [Google Scholar]
  25. Yoshida A., Yamataka A. 1953; On the metaphosphate of yeast. I. J.Biochem. (Japan) 40:85
    [Google Scholar]
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