SUMMARY: The crystal violet and iodine uptake by suspensions of and was determined. It was found that equal amounts of intracellular dye-iodine complex were formed in these micro-organisms. The molar ratio dye to iodine was 1:2. The extractability of this complex with methanol and ethanol was compared with the relative solubility of dye-iodine precipitates. Results with suggest that the solvents pass freely into the cells and decolorize them after dissociation of the dye-iodine complex. Gram-positive micro-organisms may be arranged in a number of groups according to their extractability pattern with regard to the complex. These patterns suggest the presence of interaction between the cells and ethanol. This interaction may be located at the cell wall.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error