The Minimal Amino Acid Requirements of Haemophilus pertussis

SUMMARY: Of 13 sulphur-containing compounds tested only cystine, cysteine and glutathione satisfied the sulphur requirements of the strains of Haemophilus pertussis used when grown in a defined liquid medium containing 15 amino acids. Very small inocula (3-10 viable organisms) of 8 strains grew in a medium containing glutamic acid + cystine as the only amino acids added. Four strains were carried through 9 subcultures in this medium.