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Summary: Of 13 sulphur-containing compounds tested only cystine, cysteine and glutathione satisfied the sulphur requirements of the strains of Haemophilus pertussis used when grown in a defined liquid medium containing 15 amino acids. Very small inocula (3–10 viable organisms) of 8 strains grew in a medium containing glutamic acid + cystine as the only amino acids added. Four strains were carried through 9 subcultures in this medium.
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