1887

Abstract

SUMMARY: An account is given of the development of a reliable method for the isolation of ( White), an organism associated with European foul-brood disease of the larval honeybee. , isolated as an anaerobe, may be trained to grow as an aerobe in rod form. Its principal anaerobic growth requirements are a low molar ratio of Na: K, high inorganic phosphate concentration, glucose or fructose, and undetermined factors provided by yeast extract. Peptones are harmful to growth. Aerobic growth has no very critical requirements other than glucose, fructose or sucrose. White which, together with , causes European foul-brood disease grows well anaerobically on a yeast extract + glucose + fructose medium; either sugar alone supports only feeble anaerobic growth. Anaerobic growth of . is also accelerated by a low molar ratio of Na: K and is inhibited by peptones. and . appear to be separate organisms; no evidence has been obtained to support claims by previous workers that is a variant of .

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/content/journal/micro/10.1099/00221287-17-1-39
1957-08-01
2021-10-22
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References

  1. Bailey L. 1956; Aetiology of European foul brood; a disease of the larval honeybee. Nature; Lond.: 1781130
    [Google Scholar]
  2. Bergey’s Manual of Determinative Bacteriology 1948, 6th ed.. p. 724 Breed R. S., Murray E. G. D., Hitchens A. P. Edited by London: Baillière, Tindall & Cox;
  3. Bishop G. H., Briggs A. P., Ronzoni E. 1925; Body fluids of the honeybee larva. II. Chemical constituents of the blood and their osmotic effects. J. biol. Chem 66:77
    [Google Scholar]
  4. Burri R. 1943; Weitere Beobachtungen über Formwandlungen beim Erreger der Sauerbrut der Bienen. Beih. Schweiz. Bienenztg 1:209
    [Google Scholar]
  5. Gubler H. U. 1954; Bakteriologische Untersuchungen iiber die gutartige Faulbrut der Honigbiene (Apis mellifica L.). Schweiz. Z. allg. Path 17:507
    [Google Scholar]
  6. Lochhead A. G. 1928; The etiology of European foul brood of bees. Science 67:159
    [Google Scholar]
  7. MacLeod R. A., Snell E. E. 1947; Some mineral requirements of the lactic acid bacteria. J. biol. Chem 170:351
    [Google Scholar]
  8. MacLeod R. A., Snell E. E. 1948; The effect of related ions on the potassium requirements of lactic acid bacteria. J. biol. Chem 176:39
    [Google Scholar]
  9. Report of the Rothamsted Experimental Station 1954 p. 136
  10. Steinhaus E. A. 1949 Principles of Insect Pathology, 1st ed.. p. 244 New York:: McGraw-Hill;
    [Google Scholar]
  11. Tarr H. L. A. 1937; Studies on European foul brood of bees. III. Further experiments on the production of the disease. Ann. appl. Biol 24:614
    [Google Scholar]
  12. White G. F. 1912; The cause of European foul brood. Dep. Circ. U.S. Dep. Agric57
    [Google Scholar]
  13. White G. F. 1920; European foul brood. Dep. Bull. U.S. Dep. Agric810
    [Google Scholar]
  14. Wills A. P. 1956; Sodium and potassium in Australian foods. Aust. J. exp. Biol, mod. Sci 34:165
    [Google Scholar]
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