1887

Abstract

Components of the ATP-dependent Clp protease complex are found in a wide range of prokaryotic cells and they are often expressed as part of the cellular stress response. To investigate the physiological role of the proteolytic subunit, ClpP, in serovar Typhimurium () an in-frame deletion of the gene was constructed. Growth experiments revealed that is important for the ability of to grow under various stressful conditions, such as low pH, elevated temperature and high salt concentrations. Since the stationary-phase sigma factor, RpoS, is a target of the Clp proteolytic complex, the effect of the deletion in the absence of RpoS was examined; it was observed that growth of the mutant is affected in both an RpoS-dependent and an RpoS-independent manner. Analysis of the degradation of abnormal puromycyl-containing polypeptides showed that ClpP participates in the proteolysis of such proteins in . These findings prompted an investigation of the growth of an mutant under various stress conditions. The growth of this mutant was affected by heat, salt and low pH, although not to the same extent as observed for the mutant. The results of this study indicate that the mutant is generally more sensitive to environmental stress than the mutant and it is proposed that this is due to a reduced ability to degrade misfolded proteins generated under these conditions.

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2002-09-01
2020-03-29
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