1887

Abstract

An important step in infections associated with biliary drains is adhesion of micro-organisms to the surface. In this study the role of three surface proteins of (enterococcal surface protein, aggregation substances 1 and 373) in the adhesion to silicone rubber, fluoro-ethylene-propylene and polyethylene was examined. Four isogenic strains with and without aggregation substances and one strain expressing enterococcal surface protein were used. The kinetics of enterococcal adhesion to the materials was measured in a parallel plate flow chamber. Initial deposition rates were similar for all strains, whereas the presence of surface proteins increased the total number of adhering bacteria. Nearest neighbour analysis demonstrated that enterococci expressing the whole sex-pheromone plasmid encoding aggregation substances 1 or 373 adhered in higher numbers through mechanisms of positive cooperativity, which means that adhesion of bacteria enhances the probability of adhesion of other bacteria near these bacteria. Enterococci with the enterococcal surface protein did not adhere through this mechanism. These findings indicate that the surface proteins play a key role in the adhesion to bile drains and bile drain associated infections.

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2002-06-01
2024-11-05
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References

  1. An Y. H., Friedman R. J. 1998; Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. J Biomed Mater Res 43:338–348 [CrossRef]
    [Google Scholar]
  2. Ben-Ari Z., Neville L., Davidson B., Rolles K., Burroughs A. K. 1998; Infection rates with and without T-tube splintage of common bile duct anastomosis in liver transplantation. Transplant Int 11:123–126 [CrossRef]
    [Google Scholar]
  3. Berti M., Candiani G., Kaufhold A., Muscholl A., Wirth R. 1998; Does aggregation substance of Enterococcus faecalis contribute to development of endocarditis?. Infection 26:48–53 [CrossRef]
    [Google Scholar]
  4. Bos R., Van der Mei H. C., Busscher H. J. 1999; Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for study. FEMS Microbiol Rev 23:179–230
    [Google Scholar]
  5. Bowen B. D., Levine S., Epstein N. 1976; Fine particles depostion in laminar flow through parallel plate and cylindrical channels. J Colloid Interface Sci 54:375–390 [CrossRef]
    [Google Scholar]
  6. Busscher H. J., Van der Mei H. C. 1995; Use of flow chamber devices and image analysis methods to study microbial adhesion. Methods Enzymol 253:455–477
    [Google Scholar]
  7. Busscher H. J., Bos R., Van der Mei H. C., Handley P. S. others 2000; Physicochemistry of microbial adhesion from an overall approach to the limits. In Physical Chemistry of Biological Interfaces pp 431–458 Edited by Baszkin A. New York, Basel: Marcel Dekker;
    [Google Scholar]
  8. Clewell D. B. 1993; Bacterial sex pheromone-induced plasmid transfer. Cell 73:9–12 [CrossRef]
    [Google Scholar]
  9. Clewell D. B., An F. Y., White B. A., Gawron-Burke C. 1985; Streptococcus faecalis sex pheromone (cAM373) also produced by Staphylococcus aureus and identification of a conjugative transposon (Tn 918 . J Bacteriol 162:1212–1220
    [Google Scholar]
  10. Coque T. M., Patterson J. E., Steckelberg J. M., Murray B. E. 1995; Incidence of hemolysin, gelatinase, and aggregation substance among enterococci isolated from patients with endocarditis and other infections and from feces of hospitalized and community-based persons. J Infect Dis 171:1223–1229 [CrossRef]
    [Google Scholar]
  11. Costerton J. W., Stewart P. S., Greenberg E. P. 1999; Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322 [CrossRef]
    [Google Scholar]
  12. Dabros T., Van de Ven T. G. M. 1982; Kinetics of coating by colloid particles. J Colloid Interface Sci 89:232–244 [CrossRef]
    [Google Scholar]
  13. De Boever E. H., Clewell D. B., Fraser C. M. 2000; Enterococcus faecalis conjugative plasmid pAM373: complete nucleotide sequence and genetic analyses of sex pheromone response. Mol Microbiol 37:1327–1341 [CrossRef]
    [Google Scholar]
  14. Doyle R. J. others 1991; Strategies in experimental microbial adhesion research. In Microbial Cell Surface Analysis – Structural and Physicochemical Methods pp 291–316 Edited by Mozes N. New York: VCH;
    [Google Scholar]
  15. Doyle R. J., Nesbitt W. E., Taylor K. G. 1982; On the mechanism of adhesion of Streptococcus sanguis to hydroxylapatite. FEMS Microbiol Lett 15:1–5 [CrossRef]
    [Google Scholar]
  16. Dunny G. M., Leonard B. A., Hedberg P. J. 1995; Pheromone-inducible conjugation in Enterococcus faecalis : interbacterial and host–parasite chemical communication. J Bacteriol 177:871–876
    [Google Scholar]
  17. Francia M. V., Haas W., Wirth R. 7 other authors 2001; Completion of the nucleotide sequence of the Enterococcus faecalis conjugative virulence plasmid pAD1 and identification of a second transfer origin. Plasmid 46:117–127 [CrossRef]
    [Google Scholar]
  18. Franke A. E., Clewell D. B. 1981; Evidence for a chromosome-borne resistance transposon (Tn 916 ) in Streptococcus faecalis that is capable of ‘conjugal’ transfer in the absence of a conjugative plasmid. J Bacteriol 145:494–502
    [Google Scholar]
  19. Galli D., Wirth R. 1991; Comparative analysis of Enterococcus faecalis sex pheromone plasmids identifies a single homologous DNA region which codes for aggregation substance. J Bacteriol 173:3029–3033
    [Google Scholar]
  20. Galli D., Wirth R., Wanner G. 1989; Identification of aggregation substances of Enterococcus faecalis cells after induction by sex pheromones.An immunological and ultrastructural investigation. Arch Microbiol 151:486–490 [CrossRef]
    [Google Scholar]
  21. Galli D., Lottspeich F., Wirth R. 1990; Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1. Mol Microbiol 4:895–904 [CrossRef]
    [Google Scholar]
  22. Hirt H., Antiporta M. H., Waters C. M., McCormick J. K., Schlievert P. M., Dunny G. M. 2000; In vivo expression and role of aggregation substance in experimental endocarditis. In Abstract of the 1st International ASM Conference on Enterococci: Pathogenesis, Biology, and Antibiotic Resistance, abstract S14 pp 15–16 Banff, Alberta, Canada: American Society for Microbiology;
    [Google Scholar]
  23. Ike Y., Craig R. A., White B. A., Yagi Y., Clewell D. B. 1983; Modification of Streptococcus faecalis sex pheromones after acquisition of plasmid DNA. Proc Natl Acad Sci USA 80:5369–5373 [CrossRef]
    [Google Scholar]
  24. Jacob A. E., Hobbs S. J. 1974; Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes . J Bacteriol 117:360–372
    [Google Scholar]
  25. Jett B. D., Huycke M. M., Gilmore M. S. 1994; Virulence of enterococci. Clin Microbiol Rev 7:462–478
    [Google Scholar]
  26. Koivusalo A., Makisalo H., Talja M., Siitonen A., Vuopio-Varkila J., Ruutu M., Hockerstedt K. 1996; Bacterial adherence and biofilm formation on latex and silicone T-tubes in relation to bacterial contamination of bile. Scand J Gastroenterol 31:398–403 [CrossRef]
    [Google Scholar]
  27. Kreft B., Marre R., Schramm U., Wirth R. 1992; Aggregation substance of Enterococcus faecalis mediates adhesion to cultured renal tubular cells. Infect Immun 60:25–30
    [Google Scholar]
  28. Molinari G., Pugliese V., Schito G. C., Guzman C. A. 1996; Bacteria involved in the blockage of biliary stents and their susceptibility to antibacterial agents. Eur J Clin Microbiol Infect Dis 15:88–92 [CrossRef]
    [Google Scholar]
  29. Murray B. E. 1990; The life and times of the Enterococcus. Clin Microbiol Rev 3:46–65
    [Google Scholar]
  30. Muscholl A., Galli D., Wanner G., Wirth R. 1993; Sex pheromone plasmid pAD1-encoded aggregation substance of Enterococcus faecalis is positively regulated in trans by traE1. Eur J Biochem 214:333–338 [CrossRef]
    [Google Scholar]
  31. Muscholl-Silberhorn A. 1999; Cloning and functional analysis of Asa373, a novel adhesin unrelated to the other sex pheromone plasmid-encoded aggregation substances of Enterococcus faecalis . Mol Microbiol 34:620–630 [CrossRef]
    [Google Scholar]
  32. Olmsted S. B., Dunny G. M., Erlandsen S. L., Wells C. L. 1994; A plasmid-encoded surface protein on Enterococcus faecalis augments its internalization by cultured intestinal epithelial cells. J Infect Dis 170:1549–1556 [CrossRef]
    [Google Scholar]
  33. Ruoslahti E. 1996; RGD and other recognition sequences for integrins. Annu Rev Cell Dev Biol 12:697–715 [CrossRef]
    [Google Scholar]
  34. Sartingen S., Rozdzinski E., Muscholl-Silberhorn A., Marre R. 2000; Aggregation substance increases adherence and internalization, but not translocation, of Enterococcus faecalis through different intestinal epithelial cells in vitro . Infect Immun 68:6044–6047 [CrossRef]
    [Google Scholar]
  35. Shankar V., Baghdayan A. S., Huycke M. M., Lindahl G., Gilmore M. S. 1999; Infection-derived Enterococcus faecalis strains are enriched in esp , a gene encoding a novel surface protein. Infect Immun 67:193–200
    [Google Scholar]
  36. Shankar N., Lockatell C. V., Baghdayan A. S., Drachenberg C., Gilmore M. S., Johnson D. E. 2001; Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection. Infect Immun 69:4366–4372 [CrossRef]
    [Google Scholar]
  37. Sjollema J., Busscher H. J. 1990; Deposition of polystyrene particles in a parallel plate flow cell. 2. Pair distribution functions between deposited particles. Colloids Surfaces 47:337–352 [CrossRef]
    [Google Scholar]
  38. Toledo-Arana A., Valle J., Solano C. 7 other authors 2001; The Enterococcal Surface Protein, Esp, is involved in Enterococcus faecalis biofilm formation. Appl Environ Microbiol 67:4538–4545 [CrossRef]
    [Google Scholar]
  39. Van der Mei H. C., Cox S. D., Geertsema-Doornbusch G. I., Doyle R. J., Busscher H. J. 1993; A critical appraisal of positive cooperativity in oral streptococcal adhesion: Scatchard analyses of adhesion data versus analyses of the spatial arrangement of adhering bacteria. J Gen Microbiol 139:937–948 [CrossRef]
    [Google Scholar]
  40. Willems R. J., Homan W., Top J. 9 other authors 2001; Variant esp gene as a marker of a distinct genetic lineage of vancomycin-resistant Enterococcus faecium spreading in hospitals. Lancet 357:853–855 [CrossRef]
    [Google Scholar]
  41. Wirth R. 1994; The sex pheromone system of Enterococcus faecalis . More than just a plasmid-collection mechanism?. Eur J Biochem 222:235–246 [CrossRef]
    [Google Scholar]
  42. Yu J. L., Andersson R., Ljungh A. 1996a; Infections associated with biliary drains. Scand J Gastroenterol 31:625–630 [CrossRef]
    [Google Scholar]
  43. Yu J. L., Andersson R., Parsson H., Hallberg E., Ljungh A., Bengmark S. 1996b; A bacteriologic and scanning electron microscope study after implantation of foreign bodies in the biliary tract in rats. Scand J Gastroenterol 31:175–181
    [Google Scholar]
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