1887

Abstract

infects humans, causing Legionnaires’ disease, from aerosols generated by domestic and environmental water sources. In aquatic environments is thought to replicate primarily in protozoa. A ‘repeats in structural toxin’ (RTX) gene, , from was identified recently that plays a role in entry and replication in human macrophages and also has the ability to infect mice. However, the role of this gene in the interaction of with environmental protozoa and its distribution in different species has not been examined. Southern analyses demonstrated that is present in all isolates tested and correlates with species that have been shown to cause disease in humans. To evaluate the importance of in the interaction with protozoa a series of studies was carried out in an environmental host for , . The gene plays a role in both adherence and entry into similar to that observed in human monocytic cells. Furthermore, it was found that is involved in intracellular survival and trafficking. In addition to demonstrating involvement of in the interaction of with host cells, these data support a role for this gene both during disease in humans and in environmental reservoirs.

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2002-06-01
2020-02-29
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