1887

Abstract

It is known that the Fur protein negatively regulates iron-uptake systems in different bacterial species, including . In this study it has been shown that the intracellular concentration of cyclic AMP (cAMP) is lower in a knockout mutant than in the wild-type strain. According to this, the expression of two cAMP-regulated genes, such as (encoding an α-aspartyl dipeptidase) and the operon, is decreased in cells in comparison with wild-type cells. Introduction of an additional mutation in , encoding a cyclic 3′,5′-cAMP phosphodiesterase, recovers wild-type intracellular cAMP concentration in the mutant. Likewise, expression of and the operon was the same in the double mutant and the wild-type strain. Moreover, these results also demonstrate that the Fur protein positively regulates the expression of the master operon governing the flagellar regulon. This positive control must be mediated by binding of the Fur protein to the promoter as indicated by the fact that this promoter tests positive in a Fur titration assay.

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2002-04-01
2024-10-13
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