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Volume 148, Issue 12

Genetics of biosynthesis and structure of the capsular exopolysaccharide from the Asian pear pathogen

The GenBank accession number for the sequence reported in this paper is AJ300463.

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Abstract

is a novel bacterial pathogen, which causes Asian pear blight and is related to , the causative agent of fire blight. produces exopolysaccharide (EPS) related to amylovoran in its sugar composition and sugar linkages. This was shown by degradation of the EPS with a viral depolymerase, and by methylation analysis and ESI/MS. The structure of the repeating units was confirmed by H-NMR spectra. The EPS of carried side chains, which were mainly terminated by acetyl and pyruvyl residues as found previously for amylovoran. On the other hand, a second side chain with glucose found for up to 65% of the repeating units of amylovoran was completely absent. The nucleotide sequences of five genes of the cluster of encoding proteins for EPS synthesis were characterized and displayed a high homology with the corresponding genes. Similar functions of the gene products are assumed. As for mutants of , a mutant of did not synthesize EPS and did not produce ooze on slices of immature pears or symptoms on pear seedlings. The mutant was complemented for EPS synthesis and virulence on pear slices with a gene cluster of that included .

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  • Published Online:
Keyword(s): amylovoran , Asian pear blight , CPC, cetylpyridinium chloride , cps genes , EPS, exopolysaccharide , fire blight , HR, hypersensitive reaction and sugar linkage
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2002-12-01
2024-04-27
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Genetics of biosynthesis and structure of the capsular exopolysaccharide from the Asian pear pathogen Erwinia pyrifoliaeThe GenBank accession number for the sequence reported in this paper is AJ300463.
Microbiology 148, 4015 (2002); https://doi.org/10.1099/00221287-148-12-4015
/content/journal/micro/10.1099/00221287-148-12-4015
/content/journal/micro/10.1099/00221287-148-12-4015
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