1887

Abstract

The gene of encodes the pseudouridine-55 (ψ55) synthase and is responsible for modifying all tRNA molecules in the cell at the U55 position. A null mutant grew normally on all growth media tested, but exhibited a competitive disadvantage in extended co-culture with its wild-type progenitor. The mutant phenotype could be complemented by both the cloned gene and by a D48C, catalytically inactive allele of . The mutant also exhibited a defect in survival of rapid transfer from 37 to 50 °C. This mutant phenotype could be complemented by the cloned gene but not by a D48C, catalytically inactive allele of . The temperature sensitivity of mutants could be enhanced by combination with a mutation in the gene, encoding an mU-methyltransferase, modifying the universal U54 tRNA nucleoside, but not by mutations in , encoding the enzyme catalysing the formation of Gm18. The mutant proteome contained altered levels of intermediates involved in biogenesis of the outer-membrane proteins OmpA and OmpX. The mutation also reduced the basal expression from two σ promoters, and P3. Three novel aspects to the phenotype of mutants were identified. Importantly the data support the hypothesis that TruB-effected ψ55 modification of tRNA is not essential, but contributes to thermal stress tolerance in , possibly by optimizing the stability of the tRNA population at high temperatures.

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2002-11-01
2020-09-26
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