1887

Abstract

Gene encodes peptidyl-tRNA hydrolase (Pth), an enzyme that cleaves peptidyl-tRNAs released abortively from ribosomes during protein synthesis. In the chromosome, is flanked by and , two genes of unknown function. Pth is essential for cell viability, especially under conditions leading to overproduction of peptidyl-tRNA. In an attempt to unveil the elements that affect expression, the transcriptional features of the region were investigated. Northern blot experiments showed that both and , the 3′-proximal gene, are cotranscribed in a bicistronic transcript. However, transcripts containing each of the individual messages were also detected. Accordingly, two transcriptional promoters were identified by primer extension experiments: one located upstream of , which presumably gives rise to both the mono and bicistronic transcripts, and the other, preceding , which generates its monocistronic message. Deletion analysis indicates that transcript stability depends on integrity. Also, a defect in RNase E activity resulted in Pth overproduction. It is proposed that RNase E processing within F in the bicistronic message limits expression.

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2002-11-01
2020-04-04
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