1887

Abstract

Alkali-extractable and water-soluble polysaccharides were purified from cell walls of five species of or its teleomorphs, and , and from , the type species of , a heterogeneous genus that belongs to the Hypocreales. Methylation and NMR analyses for determination of linkage types and structure were performed and indicated differences between the polysaccharides purified during the present study and those isolated from other nectrioid fungi, namely the presence of 5--substituted galactofuranose (→5)-Gal-(1→) in the main chain together with 2,6-di--substituted galactofuranose (→2,6)-Gal-(1→) residues in and . The polysaccharide from was similar to those obtained from the above species, although an additional residue of 6--substituted glucopyranose (→6)-Glc-(1→), was detected in some side chains. In and the polysaccharide contained an additional branching point of 5,6-di--substituted galactofuranose (→5,6)-Gal-(1→) linked to terminal -acetylglucosamine GlcNAc-(1→). These chains were linked to a small mannan core. All these polysaccharides showed major differences to the polysaccharide of , which was formed by a main chain of (1→6)-β-linked galactofuranose units almost fully branched at positions 2- by either single residues of glucopyranose or acidic chains containing glucuronic acid and mannose.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-147-7-1839
2001-07-01
2020-11-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/147/7/1471839a.html?itemId=/content/journal/micro/10.1099/00221287-147-7-1839&mimeType=html&fmt=ahah

References

  1. Ahrazem O., Prieto A., Leal J. A., Domenech J, Gómez-Miranda B., Jiménez-Barbero J., Bernabé M. 1997; Structural elucidation of acidic fungal polysaccharides isolated from the cell-wall of genera Cylindrocladium and Calonectria. Carbohydr Res303:67–72[CrossRef]
    [Google Scholar]
  2. Ahrazem O., Gómez-Miranda B., Prieto A., Barasoaı́n I., Bernabé M., Leal J. A. 1999; Structural characterisation of a cell wall polysaccharide from Penicillium vermoesenii : chemotaxonomic application. Can J Bot77:961–968
    [Google Scholar]
  3. Ahrazem O., Gómez-Miranda B., Prieto A., Barasoaı́n I., Bernabé M., Leal J. A. 2000; A characteristic water-soluble cell wall polysaccharide for Fusarium and Gibberella species. Mycol Res104:603–610[CrossRef]
    [Google Scholar]
  4. Bartnicki-Garcı́a S. 1968; Cell wall chemistry, morphogenesis and taxonomy of fungi. Annu Rev Microbiol22:87–108[CrossRef]
    [Google Scholar]
  5. Bartnicki-Garcı́a S. 1987; The cell wall: a crucial structure in fungal evolution. In Evolutionary Biology of the Fungi pp389–403 Edited by Rayner A. D. M., Brasier C. M., Moore D.. Cambridge: Cambridge University Press;
    [Google Scholar]
  6. Bissett J. 1983; Notes on Tolypocladium and related genera. Can J Bot61:1311–1329[CrossRef]
    [Google Scholar]
  7. Bitter T., Muir H. M. 1962; A modified uronic acid carbazole reaction. Anal Biochem4:330–334[CrossRef]
    [Google Scholar]
  8. Bock K., Pedersen C. 1983; 13C-NMR spectroscopy of monosaccharides. Adv Carbohydr Chem Biochem42:193–225
    [Google Scholar]
  9. Booth C. 1959; Studies on Pyrenomycetes. IV. Nectria (part 1). Mycol Pap73:1–115
    [Google Scholar]
  10. Chen G. C., Johnson B. R. 1983; Improved colorimetric determination of cell wall chitin in wood decay fungi. Appl Environ Microbiol46:13–16
    [Google Scholar]
  11. Ciucanu I., Kerek F. 1984; A simple and rapid method for the permethylation of carbohydrates. Carbohydr Res131:209–217[CrossRef]
    [Google Scholar]
  12. Domsch K. H., Gams W., Anderson T. H. 1980; Compendium of Soil Fungi vol. 1 London: Academic Press;
    [Google Scholar]
  13. Dubois M., Giller K. A., Rebers P. A., Smith F. A. 1956; Colorimetric method for determination of sugars and related substances. Anal Chem28:350–356[CrossRef]
    [Google Scholar]
  14. Gams K. W. 1968; Die systematische Stellung der Schimmelpilze Fusidium buxi und Verticillium candelabrum. Acta Bot Neerl. 17455–460[CrossRef]
  15. Gerwig G. J., Kamerling J. P., Vliegenthart J. F. G. 1979; Determination of the absolute configuration of monosaccharides in complex carbohydrates by capillary G.L.C. Carbohydr Res77:10–17
    [Google Scholar]
  16. Gómez-Miranda B., Rupérez P.. Leal J. A. 1981; Changes in chemical composition during germination of Botrytis cinerea sclerotia. Curr Microbiol6:243–246[CrossRef]
    [Google Scholar]
  17. Gómez-Miranda B.. Moya A., Leal J. A. 1988; Differences in the cell wall composition in the type species of Eupenicillium and Talaromyces . Exp Mycol12:258–263[CrossRef]
    [Google Scholar]
  18. Jikibara T., Takegawa K., Iwahara S. 1992; Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1. III. The primary structures of the acidic polysaccharides of the glycoproteins . J Biochem111:236–243
    [Google Scholar]
  19. Laine R. A., Esselman W. J., Sweeley C. C. 1972; Gas-liquid chromatography of carbohydrates. Methods Enzymol28:159–167
    [Google Scholar]
  20. Leal J. A., Bernabé M. 1998; Taxonomic applications of polysaccharides. In Chemical Fungal Taxonomy pp153–181 Edited by Frisvad J. C., Bridge P. D., Arora D. K.. New York & Hong Kong: Marcel Dekker;
    [Google Scholar]
  21. Leal J. A., Prieto A., Domenech J, Jiménez-Barbero J., Gómez-Miranda B., Bernabé M. 1996; Structural investigation of a cell-wall galactomannan from Neurospora crassa and N. sitophila. Carbohydr Res283:215–222[CrossRef]
    [Google Scholar]
  22. Leal J. A., Prieto A., Domenech J., Ahrazem O, Bernabé M, Gómez-Miranda B.. 1997; Possible chemotypes from cell-wall polysaccharides, as an aid in the systematics of the genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces . Mycol Res101:259–264
    [Google Scholar]
  23. Lee C. K., Gray G. R. 1988; A general strategy for the chemical sequencing of polysaccharides. J Am Chem Soc110:1292–1293[CrossRef]
    [Google Scholar]
  24. Messner R., Prillinger H., Altmann F., Lopandic K., Wimmer K., Weigang F, Molnár O.. 1994; Molecular characterization and application of random amplified polymorphic DNA analysis of Mrakia and Sterigmatomyces species. Int J Syst Bacteriol44:694–703[CrossRef]
    [Google Scholar]
  25. Parra E., Leal J. A., Prieto A, Jiménez-Barbero J., Bernabé M., Gómez-Miranda B. 1994; Structural investigation of two cell-wall polysaccharides of Penicillium expansum strains. Carbohydr Res257:239–248[CrossRef]
    [Google Scholar]
  26. Pfyffer G. E. 1998; Carbohydrates and their impact on fungal taxonomy. In Chemical Fungal Taxonomy pp247–261 Edited by Frisvad J. C., Bridge P. D., Arora D. K.. New York & Hong Kong: Marcel Dekker;
    [Google Scholar]
  27. Price W. J. 1972; Sample handling techniques.. In Laboratory Methods in Infrared Spectroscopy pp97–128 Edited by Miller R. G. J., Stace B. C.. London: Heyden;
    [Google Scholar]
  28. Prieto A., Leal J. A, Rupérez P., Hernández-Barranco A.. 1988; Partial characterisation of galactofuranose-containing heteropolysaccharides from the cell walls of Talaromyces helicus . Carbohydr Res177:265–272[CrossRef]
    [Google Scholar]
  29. Prieto A., Leal J. A, Bernabé M.. 1995; Isolation, purification and chemical characterization of alkali-extractable polysaccharides from the cell walls of Talaromyces species. Mycol Res99:69–75[CrossRef]
    [Google Scholar]
  30. Prieto A., Leal J. A., Poveda A., Domenech J., Ahrazem O, Jiménez-Barbero J., Gómez-Miranda B., Bernabé M. 1997; Structure of complex cell wall polysaccharides isolated from Trichoderma and Hypocrea species. Carbohydr Res304:281–291[CrossRef]
    [Google Scholar]
  31. Prillinger H., Hauska G, Dörfler C., Laaser G.. 1990; Ein Beitrag zur Systematik und Entwicklungsbiologie höherer Pilze Hefe-Typen der Basidiomyceten. Teil III: Ustilago -typ. Z Mykol56:251–278
    [Google Scholar]
  32. Prillinger H., Laaser G., Lockau W, Dörfler C.. 1991; Ein Beitrag zur Systematik und Entwicklungsbiologie höherer Pilze Hefe-Typen der Basidiomyceten. Teil II: Microbotryum -typ. Bot Acta104:5–17[CrossRef]
    [Google Scholar]
  33. Prillinger H., Oberwinkler F., Umile C., Tlachac K., Bauer R., Taufratzhofer E, Dörfler C.. 1993; Analysis of cell wall carbohydrate (neutral sugars) from ascomycetes and basidiomycetes yeast with and without derivatization. J Gen Appl Microbiol39:1–34[CrossRef]
    [Google Scholar]
  34. Rossman A. Y. 1989; A synopsis of the Nectria cinnabarina group. Mem N Y Bot Gard49:253–255
    [Google Scholar]
  35. Rossman A. Y. 1993; Nectria sensu-stricto and teleomorphs of Fusarium . In Holomorphic Hypocrealean Fungi pp149–160 Edited by Reynolds D. R., Taylor J. W.. Wallingford: CAB International;
    [Google Scholar]
  36. Rossman A. Y., Samuels G., Rogerson C. T., Lowen R. 1999; Genera of Bionectriaceae, Hypocreaceae and Nectriaceae (Hypocreales, Ascomycetes). Stud Mycol42:1–248
    [Google Scholar]
  37. Samuels G. J. 1989; Nectria and Sesquicillium . Mem N Y Bot Gard49:266–285
    [Google Scholar]
  38. Samuels G. J., Rossman A. Y. 1979; Conidia and classification of the nectrioid fungi. In The Whole Fungus: the Sexual-Asexual Synthesis pp167–182 Edited by Kendrick B.. Ottawa: National Museums of Canada;
    [Google Scholar]
  39. Samuels G. J., Seifert K. A. 1987; Taxonomic implications of variation among Hypocrealean anamorphs. In Pleomorphic Fungi: the Diversity and its Taxonomic Implications pp29–56 Edited by Sugiyama J.. Tokyo: Kodansha;
    [Google Scholar]
  40. Seifert K. A. 1985; A monograph of Stilbella and some allied Hyphomycetes. Stud Mycol27:1–235
    [Google Scholar]
  41. Taylor R. Y., Conrad H. E. 1972; Stoichiometric depolymerization of polyuronides and glycosaminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups. Biochemistry11:1383–1388[CrossRef]
    [Google Scholar]
  42. Weijman A. C. M., Golubev W. I. 1987; Carbohydrate patterns and taxonomy of yeast and yeast-like fungi. Stud Mycol30:361–371
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-147-7-1839
Loading
/content/journal/micro/10.1099/00221287-147-7-1839
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error