1887

Abstract

A putative operon encoding a probable zinc-responsive regulatory element () and components of an ABC-type transporter () have been characterized in . The gene was inactivated but apparently this did not alter Zn uptake. Expression of is at a low level under a range of ion conditions. To allow inducible expression of the operon, a construct was made placing it under the control of the IPTG-inducible P promoter. Using this approach, it was shown that is able to repress expression of the entire operon in a Zn-dependent manner, and that and are likely to be involved in high-affinity ion uptake. has no apparent role in pathogenicity in a lesion model of infection.

Keyword(s): fur , metals , regulation , transcription and zinc
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2001-05-01
2020-04-07
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