1887

Abstract

The genetic diversity of , the aetiological agent of fowl cholera, was investigated. The strain collection comprised 69 clinical isolates representing a wide spectrum of hosts and geographic origin. The three type strains for the subspecies of were also included. Avian isolates of subsp. and subsp. did not represent separate lines by II ribotyping and the two type strains of mammalian origin (porcine and cat bite) seemed to be representative of avian strains of subspp. and . By ribotyping, all subsp. strains, except one chicken isolate and the type strain, clustered together. This indicated that the bovine type strain was not representative of this subspecies and that most strains of subsp. are genetically related and may be distantly related to other isolates, including those of avian origin. By 16S rRNA and sequence comparisons of selected strains, including both isolated from birds and mammals and selected distantly related species associated with birds and mammals, it was found that is monophyletic. Extended DNA–DNA hybridizations are highly indicated since strains may exist which would connect the existing subspecies at species level. The considerable genetic diversity of fowl cholera isolates is probably related to the clonal nature of this organism, resulting in many divergent lines.

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2001-10-01
2019-10-23
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