1887

Abstract

The genetic diversity of , the aetiological agent of fowl cholera, was investigated. The strain collection comprised 69 clinical isolates representing a wide spectrum of hosts and geographic origin. The three type strains for the subspecies of were also included. Avian isolates of subsp. and subsp. did not represent separate lines by II ribotyping and the two type strains of mammalian origin (porcine and cat bite) seemed to be representative of avian strains of subspp. and . By ribotyping, all subsp. strains, except one chicken isolate and the type strain, clustered together. This indicated that the bovine type strain was not representative of this subspecies and that most strains of subsp. are genetically related and may be distantly related to other isolates, including those of avian origin. By 16S rRNA and sequence comparisons of selected strains, including both isolated from birds and mammals and selected distantly related species associated with birds and mammals, it was found that is monophyletic. Extended DNA–DNA hybridizations are highly indicated since strains may exist which would connect the existing subspecies at species level. The considerable genetic diversity of fowl cholera isolates is probably related to the clonal nature of this organism, resulting in many divergent lines.

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2001-10-01
2020-08-13
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References

  1. Aalbaek B., Eriksen L., Rimler R. B., Leifsson P. S., Basse A., Christiansen T., Eriksen E.. 1999; Typing of Pasteurella multocida from haemorrhagic septicaemia in Danish fallow deer ( Dama dama . APMIS107:913–920[CrossRef]
    [Google Scholar]
  2. Amann R., Sostak P., Ludwig W., Schleifer K. H.. 1988; Cloning and sequencing of genes encoding the beta subunits of the ATP-synthases from Enterobacter aerogenes and Flavobacterium ferrugineum . FEMS Microbiol Lett50:101–106[CrossRef]
    [Google Scholar]
  3. Angen Ø., Caugant D. A., Olsen J. E., Bisgaard M.. 1997; Genotypic relationships among strains classified under the [ Pasteurella ] haemolytica -complex as indicated by ribotyping and multilocus enzyme electrophoresis. Zentbl Bakteriol286:333–354[CrossRef]
    [Google Scholar]
  4. Biberstein E. L.. 1979; The pasteurelloses. In Handbook Series in Zoonoses pp495–514 Edited by Steele J. H.. Boca Raton, FL: CRC Press;
    [Google Scholar]
  5. Bisgaard M.. 1993; Ecology and significance of Pasteurellaceae in animals. Zentbl Bakteriol279:7–26[CrossRef]
    [Google Scholar]
  6. Bisgaard M., Houghton S. B., Mutters R., Stenzel A.. 1991; Reclassification of German, British and Dutch isolates of so-called Pasteurella multocida obtained from pneumonic calf lungs. Vet Microbiol26:115–124[CrossRef]
    [Google Scholar]
  7. Blackall P. J., Fegan N., Chew G. T., Hampson D. J.. 1998; Population structure and diversity of avian isolates of Pasteurella multocida from Australia. Microbiology144:279–289[CrossRef]
    [Google Scholar]
  8. Blackall P. J., Fegan N., Pahoff J. L.. 8 other authors 2000; The molecular epidemiology of four outbreaks of porcine pasteurellosis. Vet Microbiol72:111–120[CrossRef]
    [Google Scholar]
  9. Bowles R. E., Pahoff J. L., Smith B. N., Blackall P. J.. 2000; Ribotype diversity of porcine Pasteurella multocida from Australia. Aust Vet J78:630–635[CrossRef]
    [Google Scholar]
  10. Carter G. R.. 1955; Studies on Pasteurella multocida . I. A hemagglutination test for the identification of serological types. Am J Vet Res16:481–484
    [Google Scholar]
  11. Carter G. R.. 1984; Genus I. Pasteurella. In Bergey’s Manual of Systematic Bacteriology pp552–557 Edited by Krieg N. R., Holt J. G.. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  12. Christensen H., Olsen J. E.. 1998; Phylogenetic relationships of Salmonella based on DNA sequence comparison of atpD encoding the β subunit of ATP synthase. FEMS Microbiol Lett161:89–96
    [Google Scholar]
  13. Christensen J. P., Olsen J. E., Bisgaard M.. 1993; Ribotypes of Salmonella enterica serovar Gallinarum biovars gallinarum and pullorum . Avian Pathol22:725–738[CrossRef]
    [Google Scholar]
  14. Christensen J. P., Dietz H. H., Bisgaard M.. 1998; Phenotypic and genotypic characters of isolates of Pasteurella multocida obtained from back-yard poultry and from two outbreaks of avian cholera in avifauna in Denmark. Avian Pathol27:373–381[CrossRef]
    [Google Scholar]
  15. Christiansen K. H., Carpenter T. E., Snipes K. P., Hird D. W.. 1992; Transmission of Pasteurella multocida on California turkey premises in 1988–89. Avian Dis36:262–271[CrossRef]
    [Google Scholar]
  16. De Ley J., Mannheim W., Mutters R.. 7 other authors 1990; Inter- and intrafamilial similarities of rRNA cistrons of the Pasteurellaceae . Int J Syst Bacteriol40:126–137[CrossRef]
    [Google Scholar]
  17. Dewhirst F. E., Paster B. J., Bright P. L.. 1988; Chromobacterium , Eikenella , Kingella , Neisseria , Simonsiella , and Vitreoscilla species comprise a major branch of the beta group Proteobacteria by 16S ribosomal ribonucleic acid sequence comparison: transfer of Eikenella and Simonsiella to the family Neisseriaceae (emend.). Int J Syst Bacteriol38:258–266
    [Google Scholar]
  18. Dewhirst F. E., Paster B. J., Olsen I., Fraser G. J.. 1993; Phylogeny of the Pasteurellaceae as determined by comparison of 16S ribosomal ribonucleic acid sequences. Zentbl Bakteriol279:35–44[CrossRef]
    [Google Scholar]
  19. Fegan N., Blackall P. J., Pahoff J. L.. 1995; Phenotypic characterisation of Pasteurella multocida isolates from Australian poultry. Vet Microbiol47:281–286[CrossRef]
    [Google Scholar]
  20. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376[CrossRef]
    [Google Scholar]
  21. Heddleston K. L., Gallagher J. E., Rebers P. A.. 1972; Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis16:925–936[CrossRef]
    [Google Scholar]
  22. Hirsh D. C., Jessup D. A., Snipes K. P., Carpenter T. E., Hird D. W., McCapes R. H.. 1990; Characteristics of Pasteurella multocida isolated from waterfowl and associated avian species in California. J Wildl Dis26:204–209[CrossRef]
    [Google Scholar]
  23. Ludwig W., Neumaier J., Klugbauer N.. 9 other authors 1993; Phylogenetic relationships of Bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase β-subunit genes. Antonie Leeuwenhoek64:285–305
    [Google Scholar]
  24. Muhairwa A. P., Christensen J. P., Bisgaard M.. 2000; Investigations on the carrier rate of Pasteurella multocida in healthy commercial poultry flocks and flocks affected by fowl cholera. Avian Pathol29:133–142[CrossRef]
    [Google Scholar]
  25. Muhairwa A. P., Christensen J. P., Bisgaard M.. 2001; Relationships among Pasteurellaceae isolated from free ranging chickens and their animal contacts as determined by quantitative phenotyping, ribotyping and REA-typing. Vet Microbiol78:119–137[CrossRef]
    [Google Scholar]
  26. Mutters R., Ihm P., Pohl S., Frederiksen W., Mannheim W.. 1985a; Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis , Pasteurella canis , Pasteurella stomatis , Pasteurella anatis , and Pasteurella langaa . Int J Syst Bacteriol35:309–322[CrossRef]
    [Google Scholar]
  27. Mutters R., Piechulla K., Hinz K.-H., Mannheim W.. 1985b; Pasteurella avium (Hinz and Kunjara 1977) comb. nov. and Pasteurella volantium sp. nov. Int J Syst Bacteriol35:5–9[CrossRef]
    [Google Scholar]
  28. Olsen G. J., Matsuda H., Hagstrom R., Overbeek R.. 1994; fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci10:41–48
    [Google Scholar]
  29. Paster B. J., Dewhirst F. E.. 1988; Phylogeny of Campylobacters , Wolinellas , Bacteroides gracilis , and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int J Syst Bacteriol38:56–62[CrossRef]
    [Google Scholar]
  30. Petersen K. D., Christensen J. P., Bisgaard M.. 1998; Phenotypic and genotypic diversity of organisms previously classified as maltose positive Pasteurella multocida . Zentbl Bakteriol288:1–12[CrossRef]
    [Google Scholar]
  31. Rimler R. B., Glisson J. G.. 1997; Fowl cholera. In Diseases of Poultry pp143–159 Edited by Calnek B. W., Barnes H. J., Beard C. W., McDougald L. R., Saif Y. M.. London: Mosby-Wolfe;
    [Google Scholar]
  32. Rimler R. B., Rhoades K. R.. 1987; Serogroup F, a new capsule serogroup of Pasteurella multocida . J Clin Microbiol25:615–618
    [Google Scholar]
  33. Smith J. M.. 1995; Do bacteria have population genetics?. In Population Genetics of Bacteria pp1–12 Edited by Baumberg S., Young J. P. W., Wellington E. M. H., Saunders J. R.. Cambridge: Cambridge University Press;
    [Google Scholar]
  34. Snipes K. P., Hirsh D. C., Kasten R. W., Hansen L. M., Hird D. W., Carpenter T. E., McCapes R. H.. 1989; Use of rRNA probe and restriction endonuclease analysis to fingerprint Pasteurella multocida isolated from turkeys and wildlife. J Clin Microbiol27:1847–1853
    [Google Scholar]
  35. Vandamme P., Pot B., Gillis M., De Vos P., Kersters K., Swings J.. 1996; Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev60:407–438
    [Google Scholar]
  36. Vogel B. F., Jorgensen K., Christensen H., Olsen J. E., Gram L.. 1997; Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis. Appl Environ Microbiol63:2189–2199
    [Google Scholar]
  37. Wilson M. A., Morgan M. J., Barger G. E.. 1993; Comparison of DNA fingerprinting and serotyping for identification of avian Pasteurella multocida isolates. J Clin Microbiol31:255–259
    [Google Scholar]
  38. Wilson M. A., Duncan R. M., Nordholm G. E., Berlowski B. M.. 1995; Pasteurella multocida isolated from wild birds of North America: a serotype and DNA fingerprint study of isolates from 1978 to 1993. Avian Dis39:587–593[CrossRef]
    [Google Scholar]
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