1887

Abstract

Adherence of to cells of the respiratory tract of patients with cystic fibrosis (CF) appears to be a necessary precondition for colonization and infection. To date, no effective anti-adhesive strategy has been devised for preventing infection in CF patients. It was found in this study that adhered to respiratory epithelial cells both and . However, strains with cable-like pili (Cbl) exhibited the typical clump formation on pneumocytes, whereas non-cable piliated strains predominantly showed single-cell adherence. Dextrans (nominally 4000–10000 Da) significantly inhibited adhesion of to A549 pneumocytes. When compared on an equal weight basis, the nominally 10000 Da dextran was most inhibitory. A dose-dependent inhibitory effect (up to 80 mg ml) was observed for most strains. Dextran exerted less of an anti-adhesive effect on the two Cbl strains than on the others which were Cbl. Dextrans appeared to block the adherence in a non-specific fashion, as shown by the observations that the inhibitory effect was readily reversible and oligosaccharides composed of 2–4 glucose units with the same α-1,6 linkage were not inhibitory. The mean molecular masses of dextrans used in this study, as determined by gel filtration and MS, were approximately 10-fold lower than those indicated by the manufacturers. Our data suggest that dextran of nominal molecular mass 4000 Da at a concentration of 40 mg ml (10 mM according to manufacturer’s quoted molecular mass) or more may be useful in patients with CF to prevent colonization and infection with .

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2001-10-01
2020-04-07
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