1887

Abstract

Adherence of to cells of the respiratory tract of patients with cystic fibrosis (CF) appears to be a necessary precondition for colonization and infection. To date, no effective anti-adhesive strategy has been devised for preventing infection in CF patients. It was found in this study that adhered to respiratory epithelial cells both and . However, strains with cable-like pili (Cbl) exhibited the typical clump formation on pneumocytes, whereas non-cable piliated strains predominantly showed single-cell adherence. Dextrans (nominally 4000–10000 Da) significantly inhibited adhesion of to A549 pneumocytes. When compared on an equal weight basis, the nominally 10000 Da dextran was most inhibitory. A dose-dependent inhibitory effect (up to 80 mg ml) was observed for most strains. Dextran exerted less of an anti-adhesive effect on the two Cbl strains than on the others which were Cbl. Dextrans appeared to block the adherence in a non-specific fashion, as shown by the observations that the inhibitory effect was readily reversible and oligosaccharides composed of 2–4 glucose units with the same α-1,6 linkage were not inhibitory. The mean molecular masses of dextrans used in this study, as determined by gel filtration and MS, were approximately 10-fold lower than those indicated by the manufacturers. Our data suggest that dextran of nominal molecular mass 4000 Da at a concentration of 40 mg ml (10 mM according to manufacturer’s quoted molecular mass) or more may be useful in patients with CF to prevent colonization and infection with .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-147-10-2651
2001-10-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/147/10/1472651a.html?itemId=/content/journal/micro/10.1099/00221287-147-10-2651&mimeType=html&fmt=ahah

References

  1. Ashford, D., Dwek, P. A., Welply, J. K., Amatayakul, S., Homans, S. W., Lis, H., Taylor, G. N., Sharon, N. & Rademacher, T. W. ( 1987; ). The beta-2-d-xylose and alpha-1–3-l-fucose substituted N-linked oligosaccharides from Erythrina cristagalli lectin. Isolation, characterization and comparison with other legume lectins. Eur J Biochem 166, 311-320.[CrossRef]
    [Google Scholar]
  2. Barghouthi, S., Guerdoud, L. M. & Speert, D. P. ( 1996; ). Inhibition by dextran of Pseudomonas aeruginosa adherence to epithelial cells. Am J Respir Crit Care Med 154, 1788-1793.[CrossRef]
    [Google Scholar]
  3. Bigge, J. C., Patel, T. P., Bruce, J. A., Goulding, P. N., Charles, S. M. & Parekh, R. B. ( 1995; ). Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid. Anal Biochem 230, 229-238.[CrossRef]
    [Google Scholar]
  4. Bryan, R., Feldman, M., Jawetz, S. C., Rajan, S., DiMango, E., Tang, H. B., Scheffler, L., Speert, D. P. & Prince, A. ( 1999; ). The effects of aerosolized dextran in a mouse model of Pseudomonas aeruginosa pulmonary infection. J Infect Dis 179, 1449-1458.[CrossRef]
    [Google Scholar]
  5. Burns, J. L., Jonas, M., Chi, E. Y., Clark, D. A., Berger, A. & Griffith, A. ( 1996; ). Invasion of respiratory epithelial cells by Burkholderia (Pseudomonas) cepacia. Infect Immun 64, 4054-4059.
    [Google Scholar]
  6. Butler, S. L., Nelson, J. W., Poxton, I. R. & Govan, J. R. W. ( 1994; ). Serum sensitivity of Burkholderia (Pseudomonas) cepacia isolates from patients with cystic fibrosis. FEMS Immunol Med Microbiol 8, 285-292.[CrossRef]
    [Google Scholar]
  7. Canadian cystic fibrosis foundation medical/scientific advisory committee ( 1993; ). Epidemiology of Pseudomonas cepacia in cystic fibrosis. Can J Infect Dis 4, 163–164.
    [Google Scholar]
  8. Cronberg, S., Robertson, B., Nilsson, I. M. & Nilehn, J. E. ( 1966; ). Suppressive effect of dextran on platelet adhesiveness. Thromb Diath Haemorrh 16, 384-394.
    [Google Scholar]
  9. Feng, W., Garrett, H., Speert, D. P. & King, M. ( 1996; ). Improved clearability of cystic fibrosis sputum with dextran treatment in vitro. Am J Respir Crit Care Med 154, 1788-1793.[CrossRef]
    [Google Scholar]
  10. Finlay, W. H., Lange, C. F., King, M. & Speert, D. P. ( 2000; ). Lung delivery of aerosolized dextran. Am J Respir Crit Care Med 16, 91-97.
    [Google Scholar]
  11. Goldstein, R., Sun, L., Jiang, R. Z., Sajjan, U. S., Forstner, J. F. & Campbell, C. ( 1995; ). Structurally variant classes of pilus appendage fibers coexpressed from Burkholderia (Pseudomonas) cepacia. J Bacteriol 177, 1039-1052.
    [Google Scholar]
  12. Govan, J. R., Hughes, J. E. & Vandamme, P. ( 1996; ). Burkholderia cepacia: medical, taxonomic, and ecological issues. J Med Microbiol 45, 395-407.[CrossRef]
    [Google Scholar]
  13. Guile, G. R., Rudd, P. M., Wing, D. R., Prime, S. B. & Dwek, R. A. ( 1996; ). A rapid high-resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles. Ann Biochem 240, 210-226.[CrossRef]
    [Google Scholar]
  14. Isles, A., MacLusky, I., Corey, M., Gold, R., Prober, C., Fleming, P. & Levison, H. ( 1984; ). Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J Pediatr 104, 206-210.[CrossRef]
    [Google Scholar]
  15. Kuehn, M., Lent, K., Haas, J., Hagenzicker, J., Cervin, M. & Smith, A. L. ( 1992; ). Fimbriation of Pseudomonas cepacia. Infect Immun 60, 2002-2007.
    [Google Scholar]
  16. Kuster, B., Wheeler, S. F., Hunter, A. P., Dwek, R. A. & Harvey, D. J. ( 1997; ). Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography. Ann Biochem 250, 82-101.[CrossRef]
    [Google Scholar]
  17. Mahenthiralingam, E., Campbell, M. E., Henry, D. A. & Speert, D. P. ( 1996; ). Epidemiology of Burkholderia cepacia infection in patients with cystic fibrosis: analysis by randomly amplified polymorphic DNA fingerprinting. J Clin Microbiol 34, 2914-2920.
    [Google Scholar]
  18. Mahenthiralingam, E., Simpson, D. A. & Speert, D. P. ( 1997; ). Identification and characterization of a novel DNA marker associated with epidemic Burkholderia cepacia strains recovered from patients with cystic fibrosis. J Clin Microbiol 35, 808-816.
    [Google Scholar]
  19. Saini, L. S., Galsworthy, S. B., John, M. A. & Valvano, M. A. ( 1999; ). Intracellular survival of Burkholderia cepacia complex isolates in the presence of macrophage cell activation. Microbiology 145, 3465-3475.
    [Google Scholar]
  20. Sajjan, U. S. & Forstner, J. F. ( 1992; ). Identification of the mucin-binding adhesin of Pseudomonas cepacia isolates from patients with cystic fibrosis. Infect Immun 60, 1434-1440.
    [Google Scholar]
  21. Sajjan, U. S. & Forstner, J. F. ( 1993; ). Role of the 22-kilodalton pilin protein in binding of Pseudomonas cepacia to buccal epithelial cells. Infect Immun 61, 3157-3163.
    [Google Scholar]
  22. Sajjan, U. S., Sun, L., Goldstein, R. & Forstner, J. F. ( 1995; ). Cable (Cbl) type II pili of cystic fibrosis-associated Burkholderia (Pseudomonas) cepacia: nucleotide sequence of the cblA major subunit pilin gene and novel morphology of the assembled appendage fibers. J Bacteriol 177, 1030-1038.
    [Google Scholar]
  23. Sajjan, U. S., Sylvester, F. A. & Forstner, J. F. ( 2000; ). Cable-piliated Burkholderia cepacia binds to cytokeratin 13 of epithelial cells. Infect Immun 68, 1787-1795.[CrossRef]
    [Google Scholar]
  24. Snell, G. I., de Hoyos, A., Krajden, M., Winton, T. & Maurer, J. R. ( 1993; ). Pseudomonas cepacia in lung transplant recipients with cystic fibrosis. Chest 103, 466-471.[CrossRef]
    [Google Scholar]
  25. Speert, D. P. ( 1989; ). Prevention of severe lower respiratory infections in patients with cystic fibrosis. Semin Respir Infect 4, 266-271.
    [Google Scholar]
  26. Tablan, O. C., Chobra, T. L., Schidlow, D. V. & 7 other authors. ( 1985; ). Pseudomonas cepacia colonization in patients with cystic fibrosis: risk factors and clinical outcome. J Pediatr 107, 382–387.[CrossRef]
    [Google Scholar]
  27. Vandamme, P., Holmes, B., Vancanneyt, M. & 8 other authors. ( 1997; ). Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int J Syst Bacteriol 47, 1188–1200.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-147-10-2651
Loading
/content/journal/micro/10.1099/00221287-147-10-2651
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error