1887

Abstract

Attachment of to plastic materials of dental prostheses or to salivary macromolecules adsorbed on their surface is believed to be a critical event in the development of denture stomatitis. In an earlier study, it was shown that adhesion of to polystyrene, a model system to study the adhesion of to plastic materials, can be partially inhibited with an mAb directed against cell wall polysaccharides of . In the present study, the role of whole saliva in the adhesion of to polystyrene has been investigated, and three mAbs directed against epitopes of cell wall mannoproteins have been used to mimic the inhibitory effect observed with salivary secretory IgA (sIgA) on the adhesion of to polystyrene. In the absence of whole saliva, adherence of 3153 increased with germination. However, the presence of whole saliva enhanced the adhesion to polystyrene of 3153 yeast cells but decreased the adhesion of germinated cells. The enhancement of adhesion of yeast cells to polystyrene mediated by saliva was confirmed with an agerminative mutant of 3153. The inhibition of the adhesion of 3153 germ tubes to polystyrene was due to the salivary sIgA since sIgA-depleted saliva enhanced the adhesion of 3153 to polystyrene. The inhibitory effect mediated by sIgA was not related to the inhibition of germination but to the blockage of adhesins expressed on the cell wall surface of the germ tubes. The three mAbs studied reduced the adhesion of 3153 to polystyrene at levels equivalent to those for purified sIgA. The highest reduction in the adhesion was obtained with the IgA mAb N3B. The best results were obtained when the three mAbs were combined. The results suggest that whole saliva plays a different role in the adhesion of to polystyrene depending on the morphological phase of . These results may give new insights into the conflicting role of saliva in the adhesion of to plastic materials of dental prostheses.

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2000-09-01
2020-06-05
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References

  1. Arendorf T. M., Walker D. M.. 1987; Denture stomatitis: a review. J Oral Rehabil14:217–227[CrossRef]
    [Google Scholar]
  2. Bendel C. M., Hostetter M. K., McClellan M.. 1993; Distinct mechanisms of epithelial adhesion for Candida albicans and Candida tropicalis. Identification of the participating ligands and development of inhibitory peptides. J Clin Investig92:1840–1849[CrossRef]
    [Google Scholar]
  3. Budtz-Jörgensen E.. 1974; The significance of Candida albicans in denture stomatitis. Scand J Dent Res82:151–190
    [Google Scholar]
  4. Budtz-Jörgensen E.. 1990; Candida-associated denture stomatitis and angular cheilitis. In Oral Candidosis pp.156–183Edited by Samaranayake L. P., MacFarlane T. W.. London: Wright;
    [Google Scholar]
  5. Cannon R. D., Nand A. K., Jenkinson H. F.. 1995; Adherence of Candida albicans to human salivary components adsorbed to hydroxylapatite. Microbiology141:213–219[CrossRef]
    [Google Scholar]
  6. Casadevall A.. 1995; Antibody immunity and invasive fungal infections. Infect Immun63:4211–4218
    [Google Scholar]
  7. Casadevall A., Cassone A., Bistoni F., Cutler J. E., Magliani W., Murphy J. W., Polonelli L., Romani L.. 1998; Antibody and/or cell-mediated immunity, protective mechanisms in fungal disease: an ongoing dilemma or an unnecessary dispute?. Med Mycol36:95–105
    [Google Scholar]
  8. Cassone A., Conti S., De Bernardis F., Polonelli L.. 1997; Antibodies, killer toxins and antifungal immunoprotection: a lesson from nature?. Immunol Today18:164–169[CrossRef]
    [Google Scholar]
  9. Critchley I. A., Douglas L. J.. 1987; Isolation and partial characterization of an adhesin from Candida albicans. J Gen Microbiol133:629–636
    [Google Scholar]
  10. Delgado W., Aguirre J. M.. 1997; Las micosis orales en la era del sida. Rev Iberoam Micol14:14–22
    [Google Scholar]
  11. Edgerton M., Levine M. J.. 1992; Characterization of acquired denture pellicle from healthy and stomatitis patients. J Prosthet Dent68:683–691[CrossRef]
    [Google Scholar]
  12. Edgerton M., Scannapieco F. A., Reddy M. S., Levine M. J.. 1993; Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate. Infect Immun61:2644–2652
    [Google Scholar]
  13. Edgerton M., Raj P. A., Levine M. J.. 1995; Surface-modified poly(methyl methacrylate) enhances adsorption and retains anticandidal activities of salivary histatin 5. J Biomed Mater Res29:1277–1286[CrossRef]
    [Google Scholar]
  14. Epstein J. B., Kimura L. H., Menard T. W., Truelove E. L., Pearsall N. N.. 1982; Effects of specific antibodies on the interaction between the fungus Candida albicans and human oral mucosa. Arch Oral Biol27:469–474[CrossRef]
    [Google Scholar]
  15. Hawser S. P., Douglas L. J.. 1994; Biofilm formation by Candida species on the surface of catheter material in vitro. Infect Immun62:915–921
    [Google Scholar]
  16. Kennedy M. J.. 1988; Adhesion and association mechanisms of Candida albicans. In Current Topics in Medical Mycology pp.73–169Edited by McGinnins M. R.. New York: Springer;
    [Google Scholar]
  17. Kennedy M. J., Rogers A. L., Yancey R. J.. 1989; Environmental alteration and phenotypic regulation of Candida albicans adhesion to plastic. Infect Immun57:3876–3881
    [Google Scholar]
  18. McCourtie J., Douglas L. J.. 1981; Relationship between cell surface composition of Candida albicans and adherence to acrylic after growth on different carbon sources. Infect Immun32:1234–1241
    [Google Scholar]
  19. McCourtie J., MacFarlane T. W., Samaranayake L. P.. 1986; Effect of saliva and serum on the adherence of Candida species to chlorhexidine-treated denture acrylic. J Med Microbiol21:209–213[CrossRef]
    [Google Scholar]
  20. MacFarlane T. W.. 1990; Ecology and epidemiology of Candida. In Oral Candidosis pp.21–46Edited by Samaranayake L. P., MacFarlane T. W.. London: Wright;
    [Google Scholar]
  21. Miyakawa Y., Kuribayashi T., Kagaya K., Suzuki M., Nakase T., Fukazawa Y.. 1992; Role of specific determinants in mannan of Candida albicans serotype-A in adherence to human buccal epithelial cells. Infect Immun60:2493–2499
    [Google Scholar]
  22. Odds F. C.. 1988; Candida and Candidosis London: Baillière Tindall;
    [Google Scholar]
  23. Pontón J., Marot-Leblond A., Ezkurra P., Barturen B., Robert R., Senet J. M.. 1993; Characterization of Candida albicans cell wall antigens with monoclonal antibodies. Infect Immun61:4842–4847
    [Google Scholar]
  24. Pontón J., Bikandi J., Moragues M. D., Arilla M. C., Elósegui R., Quindós G., Fisicaro P., Conti S., Polonelli L.. 1996; Reactivity of Candida albicans germ tubes with salivary secretory IgA. J Dent Res75:1979–1985[CrossRef]
    [Google Scholar]
  25. Samaranayake L. P., McCourtie J., MacFarlane T. W.. 1980; Factors affecting the in vitro adherence of Candida albicans to acrylic surfaces. Arch Oral Biol25:603–609[CrossRef]
    [Google Scholar]
  26. San Millán R., Ezkurra P., Quindós G., Robert R., Senet J. M., Pontón J.. 1996; Effect of monoclonal antibodies directed against Candida albicans cell wall antigens on the adhesion of the fungus to polystyrene. Microbiology142:2271–2277[CrossRef]
    [Google Scholar]
  27. Tronchin G., Bouchara J. F., Robert R., Senet J. M.. 1988; Adherence of Candida albicans germ tube to plastic: ultrastructural and molecular studies of fibrillar adhesins. Infect Immun56:1987–1993
    [Google Scholar]
  28. Umazume M., Ueta E., Osaki T.. 1995; Reduced inhibition of Candida albicans adhesion by saliva from patients receiving oral cancer therapy. J Clin Microbiol33:432–439
    [Google Scholar]
  29. Vasilas A., Molina L., Hoffman M., Haidaris C. G.. 1992; The influence of morphological variation on Candida albicans adhesion to denture acrylic in vitro. Arch Oral Biol37:613–622[CrossRef]
    [Google Scholar]
  30. Vuddhhakul V., McCormack J. G., Seow W. K., Smith S. E., Thong Y. H.. 1988; Inhibition of adherence of Candida albicans by conventional and experimental antifungal drugs. J Antimicrob Chemother21:755–763[CrossRef]
    [Google Scholar]
  31. Vudhichamnong K., Walker D. M., Ryley H. C.. 1982; The effect of secretory immunoglobulin A on the in vitro adherence of the yeast Candida albicans to human oral epithelial cells. Arch Oral Biol27:617–621[CrossRef]
    [Google Scholar]
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