1887

Abstract

, isolate NE-32, is an endophyte of the Himalayan yew () that produces taxol, an important chemotherapeutic drug used in the treatment of breast and ovarian cancers. Conditions were determined to induce the perfect stage (teleomorph) of this organism in the laboratory as a critical first step to study inheritance of taxol biosynthetic genes. The perfect stage of NE-32 forms in a period of 3–6 weeks on water agarose with dried yew needles at 16–20 °C with 12 h of light per day. Morphological analysis of the teleomorph and sequencing of the 18S rDNA indicates that is the perfect stage of . Only certain plants (e.g. yews, some pines, pecan, oat and some barley cultivars) allow the production of perithecia. Exhaustive methylene chloride extraction of yew () needles removes their capacity to induce production of perithecia. The methylene chloride extract is able to induce formation of perithecia by strain NE-32 in a bioassay system utilizing the sterilized sheaths of the Cholla cactus () spine, indicating that a chemical compound(s) in yew stimulates the formation of the perfect stage. This hydrophobic plant compound(s) has been designated the perithecial-stimulating factor (PSF). The data suggest that plant products may play a role in regulating the biology of endophytic microbes.

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2000-08-01
2019-10-18
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References

  1. Barr, M. E. ( 1975; ). Pestalosphaeria, a new genus in the Amphispaeriaceae. Mycologia 67, 187-194.[CrossRef]
    [Google Scholar]
  2. Chang, H. S. & Chang, J. M. ( 1990; ). Pestalosphaeria hansenii, the teleomorph of an anamorph Pestalotiopsis sp., on mangrove (Kandelia candel (L.) Druce) leaves and its ascomatal formation under laboratory conditions. Bot Bull Acad Sin (Taipei) 31, 175-177.
    [Google Scholar]
  3. Croan, S. C., Kim-Young, H. & Kim, Y. H. ( 1997; ). Carpogenesis and basidiosporogenesis by Flammulina velutipes, Schizophyllum commune and Trametes versicolor in vitro. Mater Org (Berl) 31, 1-16.
    [Google Scholar]
  4. Dykstra, M. J. (1993). A Manual of Applied Techniques for Biological Electron Microscopy. New York: Plenum.
  5. Gooday, G. W. & Adams, D. J. ( 1993; ). Sex hormones and fungi. Adv Microb Physiol 34, 69-145.
    [Google Scholar]
  6. Hansen, H. N., Zentmeyer, G. A. & Snyder, W. C. ( 1956; ). The perfect stage of Pestalotia. Phytopathology 46, 637.
    [Google Scholar]
  7. Hendrix, J. W. ( 1964; ). Sterol induction of reproduction and stimulation of growth of Pythium and Phytophthora. Ann NY Acad Sci 144, 1028-1029.
    [Google Scholar]
  8. Hess, W. M. ( 1966; ). Fixation and staining of fungus hyphae and host plant root tissues for electron microscopy. Stain Technol 41, 27-35.
    [Google Scholar]
  9. Hess, W. M. & Seegmiller, R. E. ( 1988; ). Computerized image analysis of resin-embedded hair. Trans Am Microsc Soc 107, 421-425.[CrossRef]
    [Google Scholar]
  10. Hyde, K. D. ( 1996; ). Fungi from palms. XXV. Pestalosphaeria elaeidis. Mycotaxon 57, 353-357.
    [Google Scholar]
  11. Kang, J. C., Hyde, K. D. & Kong, R. Y. C. ( 1999; ). Studies on Amphisphaeriales: the Amphisphaeriaceae (sensu stricto). Mycol Res 103, 53-64.[CrossRef]
    [Google Scholar]
  12. Lee, S. B. & Taylor, J. W. (1990). Isolation of DNA from fungal mycelia and single spores. In PCR Protocols: a Guide to Methods and Applications, pp. 282–287. Edited by M. A. Innis and others. New York: Academic Press.
  13. Li, J. Y, Strobel, G. A., Sidhu, R. S., Hess, W. M. & Ford, E. J. ( 1996; ). Endophytic taxol-producing fungi from bald cypress, Taxodium distichum. Microbiology 142, 2223-2226.[CrossRef]
    [Google Scholar]
  14. Long, D. M., Smidansky, E. D., Archer, A. & Strobel, G. A. ( 1998; ). In vivo addition of telomeric repeats to foreign DNA generates extrachromosomal DNAs in the taxol-producing fungus Pestalotiopsis microspora. Fungal Genet Biol 24, 335-344.[CrossRef]
    [Google Scholar]
  15. Meguro, S., Takagi, N. & Imamura, H. ( 1988; ). The effects of electric impulses on fruiting body formation of Lentinus edodes. Bull Kyushu Univ For 59, 61-69.
    [Google Scholar]
  16. Mirocha, C. J. & Swanson, S. P. ( 1983; ). Regulation of perithecia production in Fusarium roseum by zearalenone. J Food Safety 5, 41-53.[CrossRef]
    [Google Scholar]
  17. Nag Raj, T. R. ( 1985; ). Redisposals and redescriptions in the Monochaetia–Seiridium, Pestalotia–Pestalotiopsis complexes. II. Pestalotiopsis besseyii (Guba) comb. nov. and Pestalosphaeria varia sp. nov. Mycotaxon 22, 52-63.
    [Google Scholar]
  18. Nelson, T. A., Toussoun, T. & Marasas, W. F. O. (1983). Fusarium species. University Park, PA: Penn State University Press.
  19. Sathiarajan, P. K. & Govindan, M. ( 1989; ). Leaf blight of coconut caused by Pestalospaeria elaeidis (Booth & Robertson) Vander AA – a new record. Agric Res J Kerala 27, 61-62.
    [Google Scholar]
  20. Shoemaker, R. A. & Simpson, J. A. ( 1981; ). A new species of Pestalosphaeria on pine (Pinus) with comments on the generic placement of the anamorph. Can J Bot 59, 986-999.[CrossRef]
    [Google Scholar]
  21. Siddiq, A. A., Ingram, D. S., Johnstone, K., Friend, J. & Ashby, A. M. ( 1989; ). The control of asexual and sexual development by morphogens in fungal pathogens. Asp Appl Biol 23, 417-426.
    [Google Scholar]
  22. Spurr, A. R. ( 1969; ). A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26, 31-43.[CrossRef]
    [Google Scholar]
  23. Strobel, G., Yang, X., Sears, J., Kramer, R., Sidhu, R. S. & Hess, W. M. ( 1996a; ). Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiology 142, 435-440.[CrossRef]
    [Google Scholar]
  24. Strobel, G. A., Hess, W. M., Ford, E. J., Sidhu, R. S. & Yang, X. ( 1996b; ). Taxol from fungal endophytes and the issue of biodiversity. J Ind Microbiol 17, 417-423.[CrossRef]
    [Google Scholar]
  25. Tan, Y. H. & Chang, S. T. (1989). Effect of growth regulators, enzyme inhibitors and stimulatory additives on the vegetative development and fructification of Lentinus edodes. In Mushroom Science, Pt II: Proceedings of the 12th International Congress on the Science and Cultivation of Edible Fungi, pp. 267–277.
  26. Upadhyay, R. V., Strobel, G. A. & Hess, W. M. ( 1991; ). Morphogenesis and ultrastructure of the conidiomata of Aschochyta cypericola. Mycol Res 95, 785-791.[CrossRef]
    [Google Scholar]
  27. Valent, B. & Chumley, F. G. ( 1991; ). Molecular genetic analysis of the rice blast fungus, Magnaporthe grisea. Annu Rev Phytopathol 29, 443-467.[CrossRef]
    [Google Scholar]
  28. Wolf, J. C. & Mirocha, C. J. ( 1977; ). Control of sexual reproduction in Gibberella zeae (Fusarium roseum ‘‘Graminearum’’) Appl Environ Microbiol 33, 546-550.
    [Google Scholar]
  29. Yildiz, A. ( 1998; ). Effects of various proportions of different additive substances on the micelle growth of Pleurotis florida Fovose, on duration of formation and development of basidiocarps and on the yield. Turk J Biol 22, 127-142.
    [Google Scholar]
  30. Zhu, P., Ge, Q. & Xu, T. ( 1991; ). The perfect stage of Pestalotiopsis from China. Mycotaxon 40, 129-140.
    [Google Scholar]
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