1887

Abstract

The , and genes of pv. are essential for ferric iron uptake. In contrast, the gene located in the same gene cluster is not essential. Mutational analysis revealed that the ferric-iron-uptake genes , and are necessary for the induction of a hypersensitive response (HR) on the nonhost plant pepper () and the induction of typical black rot symptoms on the host plant cauliflower (). Again, the gene behaved differently. It was found to play a role only in the induction of the HR in pepper but not in the induction of black rot symptoms in cauliflower. Due to the low iron concentration in the plant tissue, the titre of viable bacteria of the ferric-iron-uptake mutants , and decreased after leaf infiltration of pepper. The mutant, however, which is not impaired in ferric iron uptake, multiplied in the pepper leaf tissue and grew even better than the wild-type strain, probably due to its failure to induce the HR. Nevertheless, the , and mutant strains were able to spread systemically in cauliflower.

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2000-05-01
2020-09-29
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References

  1. Aguilar O. M., Kapp D., Pühler A.. 1985; Characterization of a Rhizobium meliloti fixation gene (fixF) located near the common nodulation region. J Bacteriol164:245–254
    [Google Scholar]
  2. Arlat M., Gough C. L., Barber C. E., Boucher C., Daniels M. J.. 1991; Xanthomonas campestris contains a cluster of hrp genes related to the larger hrp cluster of Pseudomonas solanacearum. Mol Plant–Microbe Interact4:593–601[CrossRef]
    [Google Scholar]
  3. Arlat M., Van Gijsegem F., Huet J. C., Pernollet J. C., Boucher C. A.. 1994; PopA1, a protein which induces a hypersensitive-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum. EMBO J13:543–553
    [Google Scholar]
  4. Arnold W., Pühler A.. 1988; A family of high copy-number plasmid vectors with single end-label sites for rapid nucleotide sequencing. Gene70:171–179[CrossRef]
    [Google Scholar]
  5. Bachmann K., Patschne M., Gilbert W.. 1976; Construction of plasmids carrying the CI gene of bacteriophage λ. Proc Natl Acad Sci USA73:4174–4178[CrossRef]
    [Google Scholar]
  6. Bonas U.. 1994; hrp genes of phytopathogenic bacteria. Curr Top Microbiol Immunol192:79–98
    [Google Scholar]
  7. Bradbeer C.. 1993; The proton motive force drives the outer membrane transport of cobalamin in Escherichia coli. J Bacteriol175:3146–3150
    [Google Scholar]
  8. Braun V.. 1995; Energy-coupled transport and signal transduction through the gram-negative outer membrane via TonB-ExbB-ExbD-dependent receptor proteins. FEMS Microbiol Rev16:295–307[CrossRef]
    [Google Scholar]
  9. Conrads-Strauch J., Dow J. M., Milligan D. E., Parra R., Daniels M. J.. 1990; Induction of hydrolytic enzymes in Brassica campestris in response to pathovars of Xanthomonas campestris. Plant Physiol93:238–243[CrossRef]
    [Google Scholar]
  10. Dangl J. L.. 1994; The enigmatic avirulence genes of phytopathogenic bacteria. Curr Top Microbiol Immunol192:99–118
    [Google Scholar]
  11. Daniels M. J., Barber C. E., Dow J. M., Han B., Liddel S. A., Newman M. A., Parker J. E., Soby S. D., Wilson T. G. J.. 1992; Plant and bacterial genes involved in interactions between Xanthomonas and crucifers. In Advances in Molecular Genetics of Plant–Microbe Interactions pp.423–433Edited by Nester E. W., Verma D. P. S.. Dordrecht: Kluwer;
    [Google Scholar]
  12. Dow J. M., Daniels M. J.. 1994; Pathogenicity determinants and global regulation of pathogenicity of Xanthomonas campestris pv. campestris. Curr Top Microbiol Immunol192:29–41
    [Google Scholar]
  13. Enard C., Diolez A., Expert D.. 1988; Systemic virulence of Erwinia chrysanthemi 3937 requires a functional iron assimilation system. J Bacteriol170:2419–2426
    [Google Scholar]
  14. Expert D., Enard C., Masclaux C.. 1996; The role of iron in plant host–pathogen interactions. Trends Microbiol4:232–237[CrossRef]
    [Google Scholar]
  15. Fenselau S., Bonas U.. 1995; Sequence and expression analysis of the hrpB pathogenicity operon of Xanthomonas campestris pv. vesicatoria which encodes eight proteins with similarity to components of the Hrp, Ysc, Spa, and Fli secretion system. Mol Plant–Microbe Interact8:845–854[CrossRef]
    [Google Scholar]
  16. Fenselau S., Balbo I., Bonas U.. 1992; Determinants of pathogenicity in Xanthomonas campestris pv. vesicatoria are related to proteins involved in secretion in bacterial pathogens of animals. Mol Plant–Microbe Interact5:390–396[CrossRef]
    [Google Scholar]
  17. Härle C., Kim I., Angerer A., Braun V.. 1995; Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface. EMBO J145:1430–1438
    [Google Scholar]
  18. He S. Y., Huang H. C., Collmer A.. 1993; Pseudomonas syringae pv. syringae harpinPss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants. Cell73:1255–1266[CrossRef]
    [Google Scholar]
  19. Hötte B., Rath-Arnold I., Pühler A., Simon R.. 1990; Cloning and analysis of a 35·3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris. J Bacteriol172:2804–2807
    [Google Scholar]
  20. Klement Z.. 1982; Hypersensitivity. In Phytopathogenic Prokaryotes pp.149–177Edited by Mount M. S., Lacy G. H.. New York: Academic Press;
    [Google Scholar]
  21. Koster M., van Klompenburg W., Bitter W., Leong J., Weisbeck P.. 1994; Role for the outer membrane ferric siderophore receptor PupB in signal transduction across the bacterial cell envelope. EMBO J13:2805–2813
    [Google Scholar]
  22. Loper J. E., Buyer J. S.. 1991; Siderophores in microbial interactions on plant surfaces. Mol Plant–Microbe Interact4:5–13[CrossRef]
    [Google Scholar]
  23. Maniatis T., Fritsch E. F., Sambrook J.. 1982; Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Meade H., Long S., Ruvkun G., Brown S., Ausubel F.. 1982; Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J Bacteriol149:114–122
    [Google Scholar]
  25. Morrison D. A.. 1977; Transformation in E. coli: cryogenic preservation of competent cells. J Bacteriol132:349–351
    [Google Scholar]
  26. Postle K.. 1990; TonB and the gram-negative dilemma. Mol Microbiol4:2019–2025[CrossRef]
    [Google Scholar]
  27. Raymond K. N., Müller G. I., Matzanke B. F.. 1989; Complexation of iron by siderophores. A review of their solution and structural chemistry and biological function. Top Curr Chem123:49–58
    [Google Scholar]
  28. Ritter C., Dangl J. L.. 1995; The avrRpm1 gene of Pseudomonas syringae pv. maculicola is required for virulence on Arabidopsis. Mol Plant–Microbe Interact8:444–453[CrossRef]
    [Google Scholar]
  29. Simon R., Hötte B., Klauke B., Kosier B.. 1991; Isolation and characterization of insertion sequence elements from Gram-negative bacteria using new broad-host-range, positive selection vectors. J Bacteriol173:1502–1508
    [Google Scholar]
  30. Sutherland I. W.. 1993; Xanthan. In Xanthomonas pp.363–388Edited by Swings J. G., Civerolo E. L.. London: Chapman & Hall;
    [Google Scholar]
  31. Swings J. G., Civerolo E. L.. 1993; Xanthomonas London: Chapman & Hall;
    [Google Scholar]
  32. Van den Ackerveken G., Marois E., Bonas U. . 1996; Recognition of the bacterial avirulence protein AvrBs3 occurs inside the host plant cell. Cell87:1307–1316[CrossRef]
    [Google Scholar]
  33. Wei Z.-M., Laby R. J., Zumoff C. H., Bauer D. W., He S. Y., Collmer A. C., Beer S. V.. 1992; Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. Science257:85–88[CrossRef]
    [Google Scholar]
  34. Wengelnik K., Marie C., Russel M., Bonas U.. 1996; Expression and localization of HrpA1, a protein of Xanthomonas campestris pv. vesicatoria essential for pathogenicity and induction of the hypersensitive reaction. J Bacteriol178:1061–1069
    [Google Scholar]
  35. Wiggerich H. G., Klauke B., Köplin R., Priefer U. B., Pühler A.. 1997; Unusual structure of the tonB-exb DNA region of Xanthomonas campestris pv. campestris: tonB, exbB, and exbD1 are essential for ferric iron uptake, but exbD2 is not. J Bacteriol179:7103–7110
    [Google Scholar]
  36. Williams P. H.. 1980; Black rot, a continuing threat to world crucifers. Plant Dis64:736–742[CrossRef]
    [Google Scholar]
  37. Willis D. K., Rich J. J., Hrabak E. M.. 1991; hrp genes of phytopathogenic bacteria. Mol Plant–Microbe Interact4:132–138[CrossRef]
    [Google Scholar]
  38. Yang Y., Gabriel D. W.. 1995; Xanthomonas avirulence/pathogenicity gene family encodes functional plant nuclear targeting signals. Mol Plant–Microbe Interact8:627–631[CrossRef]
    [Google Scholar]
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