1887

Abstract

The isolation and functional characterization of a Na/H antiporter gene, is reported here. The gene encodes a protein of 840 amino acids that exhibits high levels of similarity in sequence, size, and structural and functional domains to a group of known Na/H antiporters of fungi. The gene is able to functionally complement the salt-sensitivity of a mutant, and mutations of two conserved aspartate residues to asparagines in the putative Na-binding site abolish this activity. Deletion of results in retardation of growth and a highly elongated morphology in a significant fraction of cells under conditions that normally support yeast growth. These results indicate that has a role in Na and H transport, salt-tolerance, and morphogenesis.

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2000-05-01
2024-12-03
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