1887

Abstract

As a basis for studing the essential cellular processes of hyperthermophilic archaea, thermosensitive mutants of were isolated and characterized. Exponential-phase liquid cultures were shifted to the non-permissive temperature and growth, viability, and distributions of cell mass and DNA content were measured as a function of time after the shift. The observed phenotypes demonstrate that chromosome replication, nucleoid organization, nucleoid partition and cell division, which normally are tightly co-ordinated during cellular growth, can be inhibited or uncoupled by mutation in this hyperthermophilic archaeon.

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2000-03-01
2020-01-20
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