1887

Abstract

The ability of mRNA to direct synthesis of cDNA in the presence of oligo(dT) was analysed using a novel application of fluorescein-11-dUTP incorporation into cDNA by reverse transcriptase. Evidence is provided for the first time that a majority of the mycobacterial mRNA pool is polyadenylylated. mRNA transcripts of were also amplified with specific primers from the oligo(dT)-primed cDNA preparation in BCG, and . Furthermore, PCR amplication of cDNAs for genes , and from BCG yielded the expected products when reverse transcription was primed with oligo(dT), suggesting that polyadenylylation is a general phenomenon in mycobacteria.

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2000-03-01
2020-01-21
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