1887

Abstract

Cyanobacteria modulate intracellular levels of cAMP and cGMP in response to environmental conditions (light, nutrients and pH). In an attempt to identify components of the cAMP and cGMP signalling pathways in PCC 6803, the authors screened its complete genome sequence by using bioinformatic tools and data from sequence–function studies performed on both eukaryotic and prokaryotic cAMP/cGMP-dependent proteins. Sll1624 and Slr2100 were tentatively assigned as being two putative cyclic nucleotide phosphodiesterases. Five proteins were identified as having all the determinants required to be cyclic nucleotide receptors, two of them being probably more specific for cGMP (an element of two-component regulatory systems – Slr2104 – and a putative cyclic-nucleotide-gated cation channel – Slr1575), the three others being probably more specific for cAMP: (i) a protein of unidentified function (Slr0842); (ii) a putative cyclic-nucleotide-modulated permease (Slr0593), previously annotated as a kinase A regulatory subunit; and (iii) a putative transcription factor (CRP- =Sll1371), which possesses cAMP- and DNA-binding determinants homologous to those of the cAMP receptor protein of (CRP-). This homology, together with the presence in of CRP--like binding sites upstream of , , , and several genes encoding enzymes involved in transport and metabolism, strongly suggests that CRP- is a global regulator.

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2000-12-01
2020-01-21
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