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Volume 146, Issue 11

Phylogenetic diversity of rhizobial strains nodulating L. Free

Abstract

The EMBL accession numbers for the sequences reported in this paper are AJ271898–AJ271902 for the strains Rob6, Rob8 and Rob23 and the strains Rob18 and Rob20, respectively.

Lack of knowledge exists regarding the diversity of rhizobial strains nodulating black locust ( L.), which is a neophytic tree species widely distributed in Europe. Seventeen rhizobial strains isolated from nodules of black locust at a German location were examined by phenotypic characterization and 16S rDNA analysis. The isolates were classified in nine 16S rDNA genotypes using a set of seven endonucleases. Based on RFLP analysis and sequencing, the strains were shown to belong to the genera (76%) and (24%). Five genotypes were identical to the species , , , and . A strong similarity between the 16S rDNA sequence of another two genotypes and (999%) as well as the strain R88b (998%) was found. The two remaining genotypes were classified in the genus , without a significant relationship at the species level. Comparing isolates nodulating and , a parallel picture of phylogenetic diversity was detected with a range of phylogenetically different rhizobia and dominating. For this study, 18 rhizobial strains which had originally been isolated from a forest in Maryland where black locust is native were additionally analysed. Results revealed seven genotypes all belonging to the genus , with four genotypes identical to the isolates from the German sampling location. Whereas the genotype identical to dominated within the strains obtained from the German location, the dominance of a genotype identical to was found among the strains from the native location. Summarizing data from both locations, was nodulated with various genomic species, most of which belonged to the genus . Concerning phenotypic features such as growth rate, pH tolerance or use of certain carbohydrates, most isolates corresponded to described species and genera. However, there were differences in salt tolerance between these isolates and the corresponding reference strains. Overall, the results demonstrated a high phenotypic and phylogenetic diversity of rhizobial strains nodulating . This may be a characteristic of neophytic and other widely spread legumes and may contribute to the success of black locust as a pioneer tree species for the temperate zone.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): 16S rDNA sequences , black locust , Mesorhizobium , PCR-RFLP and Robinia pseudoacacia
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2000-11-01
2024-04-26
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Phylogenetic diversity of rhizobial strains nodulating Robinia pseudoacacia L.
Microbiology 146, 2997 (2000); https://doi.org/10.1099/00221287-146-11-2997
/content/journal/micro/10.1099/00221287-146-11-2997
/content/journal/micro/10.1099/00221287-146-11-2997
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