1887

Abstract

grown for 16 h in the presence of [C]spermine formed a high intracellular pool of [C]spermidine and a small but detectable pool of [C]putrescine. When [H]putrescine was added to the growth medium, a large intracellular pool of [H]putrescine was found, but it was not further metabolized, confirming previous studies suggesting the absence of a forward-directed polyamine synthetic pathway in . Spermidine:spermine -acetyltransferase (SSAT) and polyamine oxidase enzyme activities were detected which collectively converted spermine to spermidine. Polyamine oxidase was localized in the hydrogenosome-enriched fraction, whereas SSAT was found predominantly in the cytosolic fraction. In the presence of saturating substrate, the trichomonad SSAT had an activity of 039±009 nmol min (mg protein) (the mean of five analyses) and an apparent for spermine of 17 μM. The enzyme was competitively inhibited by di(ethyl)spermine with a of 28 μM. Growth studies indicated that 50 μM di(ethyl)spermine caused a 68% and 84% reduction in the intracellular concentrations of spermidine and spermine, respectively. The trichomonad polyamine oxidase required FAD as a cofactor and had an apparent of 60 μM for -acetylspermine. The potential of bis(alkyl) polyamine analogues as antitrichomonad agents is discussed.

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2000-10-01
2020-03-29
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