1887

Abstract

The secretion-dedicated chaperone SecB targets a subset of proteins to the translocase by interacting with the carboxyl (C-) terminus of SecA. This region of SecA is highly conserved in Eubacteria, but despite its presence in the SecA, the genome does not appear to contain a gene for a clear homologue of SecB. Deletion of the C-terminus of the SecA yields cells that have normal viability, but that exhibit a response reminiscent of oxidative stress and the loss of a number of secretory proteins from the culture supernatant. Semi-quantitative RT-PCR demonstrates that these proteins are expressed at lower levels. The C-terminus of SecA fused to glutathione -transferase (GST) specifically binds a cytosolic protein, termed MrgA. This protein has been reported to function in relation to oxidative stress, but deletion of the gene does not result in a secretion defect nor does it cause an oxidative stress response. It is concluded that the C-terminus of the SecA is not essential for secretion and viability.

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2000-10-01
2020-04-04
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