1887

Abstract

An internal adenylyltransferase gene () fragment from was amplified using heterologous PCR primers derived from consensus motifs. The sequence had significant similarity to bacterial genes, and included a motif typical of the C-terminal adenylyltransferase domain of GlnE. from lies on the I-C fragment of the chromosome and is localized near (encoding glutamine synthetase I, GSI) and (encoding GSII). To analyse the function of GlnE in , ( E4) and ( HT107) gene replacement mutants were constructed. The GSI activity of the mutant was not down-regulated after an ammonium shock. However, the GSI activity of the wild-type cells decreased to 60% of the original activity. The mutant is not glutamine auxotrophic, but in the γ-glutamyltransferase assay no GSI activity was detected in unshifted and shifted HT107 cells. By snake venom phosphodiesterase treatment the GSI activity in the wild-type can be reconstituted, whereas no alteration is observed in the E4 mutant. Additionally, the loss of short-term GSI regulation in the E4 mutant was accompanied by an increased glutamine:glutamate ratio.

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1999-09-01
2020-11-30
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