1887

Abstract

The population structure of in a sample of 134 carried antibiotic-susceptible isolates, and 53 resistant and susceptible invasive isolates, was examined using a DNA-based version of multilocus enzyme electrophoresis: multilocus restriction typing (MLRT). This involved RFLP analysis of PCR products generated from nine loci of housekeeping genes located around the pneumococcal chromosome. The combination of alleles at each of the nine loci gave an allelic profile or restriction type (RT). All carried (throat or nasopharyngeal) isolates from children or adults in Oxford and Manchester, UK, and from an HIV-seropositive cohort in Nairobi, Kenya, showed an epidemic population structure. Twelve carried clonal groups, each with different serotypes, were identified at both locations within the UK. Almost all of the carried clones examined (16/17) were found to possess identical RTs or sequence types (STs) to invasive isolates, indicating that frequently carried clones are also associated with cases of invasive disease. As expected from previous studies, the population of 53 invasive, mainly penicillin-resistant, isolates was also found to be at linkage equilibrium. Serotype switching was identified among 14% of RTs that possessed two or more members, or 57% of individual isolates within these RTs. In support of a population structure in which there is frequent recombination, there is also clear evidence that the locus within pneumococci has evolved by horizontal gene transfer. A non-serotypable isolate from an HIV-seropositive patient in Kenya was clearly genetically distinct from other strains studied, with unique alleles at eight out of nine loci examined. However, it was initially identified as a pneumococcus by a 16S RNA gene probe (Gen-Probe), optochin susceptibility and the presence of pneumolysin and autolysin.

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1999-11-01
2024-12-08
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References

  1. Appelbaum P. C., Gladkova C., Hryniewicz W.10 other authors 1996; Carriage of antibiotic resistant Streptococcus pneumoniae by children in Eastern and Central Europe – a multicenter study with use of standardised methods. Clin Infect Dis 23:712–717 [CrossRef]
    [Google Scholar]
  2. Austrian R. 1986; Some aspects of the pneumococcal carrier state. J Antimicrob Chemother 18:35–45 [CrossRef]
    [Google Scholar]
  3. Austrian R., Douglas R. M., Schiffman G.6 other authors 1976; Prevention of pneumococcal pneumonia by vaccination. Trans Assoc Am Phys 89:184–194
    [Google Scholar]
  4. Baquero F., Jesus B. 1997; Evolution of antibiotic resistance. Trends Ecol Evol 12:482–487 [CrossRef]
    [Google Scholar]
  5. Brown A. H. D., Feldman M. W., Nevo E. 1980; Multilocus structure of natural populations of Hordeum spontaneum. Genetics 96:523–536
    [Google Scholar]
  6. Coffey T. J., Dowson C. G., Daniels M., Zhou J., Martin C., Spratt B. G., Musser J. M. 1991; Horizontal transfer of multiple penicillin-binding protein genes and capsular biosynthetic genes, in natural populations of Streptococcus pneumoniae. Mol Microbiol 5:2255–2260 [CrossRef]
    [Google Scholar]
  7. Coffey T. J., Enright M. C., Daniels M., Morona J. K., Morona R., Hryniewicz W., Paton J. C., Spratt B. G. 1998; Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae. Mol Microbiol 27:73–83 [CrossRef]
    [Google Scholar]
  8. Diaz E., Lopez R., Garcia J. L. 1992; Role of the major pneumococcal autolysin in the atypical response of a clinical isolate of Streptococcus pneumoniae. . J Bacteriol 174:5508–5515
    [Google Scholar]
  9. Dowson C. G., Coffey T. J., Kell C., Whiley R. A. 1993; Evolution of penicillin resistance in Streptococcus pneumoniae; the role of Streptococcus mitis in the formation of a low affinity PBP2B in S. pneumoniae. Mol Microbiol 9:635–643 [CrossRef]
    [Google Scholar]
  10. Enright M. C., Spratt B. G. 1998; A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology 144:3049–3060 [CrossRef]
    [Google Scholar]
  11. Feldman C., Klugman K. P. 1997; Pneumococcal infections. Curr Opin Infect Dis 10:109–115 [CrossRef]
    [Google Scholar]
  12. Guttman D. S. 1997; Recombination and clonality in natural populations of Escherichia coli. Trends Ecol Evol 12:16–21
    [Google Scholar]
  13. Hall L. M. C., Whiley R. A., Duke B., George R. C., Efstratiou A. 1996; Genetic relatedness within and between serotypes of Streptococcus pneumoniae from the United Kingdom: analysis of multilocus enzyme electrophoresis, pulsed-field gel electrophoresis, and antimicrobial resistance patterns. J Clin Microbiol 34:853–859
    [Google Scholar]
  14. Haubold B., Rainey P. B. 1996; Genetic and ecotypic structure of a fluorescent Pseudomonas population. Mol Ecol 5:747–761 [CrossRef]
    [Google Scholar]
  15. Hermans P. W., Sluijter M., Dejsirilert S., Lemmens N., Elzenaar K., van Veen A., Goessens W. H., deGroot R. 1997; Molecular epidemiology of drug-resistant pneumococci: towards an international approach. Microb Drug Resist 3:243–251 [CrossRef]
    [Google Scholar]
  16. Hendley O. J., Sande M. A., Stewart P. M., Gwaltney J. M. 1975; Spread of Streptococcus pneumoniae in families. I. Carriage rates and distribution of types. J Infect Dis 132:55–61 [CrossRef]
    [Google Scholar]
  17. Hoeprich P. D. 1994; Bacterial pneumonias. In Infectious Diseases pp. 421–433Edited by Hoeprich P. D., Jordan M. C., Ronald A. R. Philadelphia: Lippincott;
    [Google Scholar]
  18. Jordens Z., Paul J., Bates J., Beaumont C., Kimari J., Gilks C. 1995; Characterization of Streptococcus pneumoniae from human immunodeficiency virus – seropositive patients with acute and recurrent pneumonia. J Infect Dis 172:983–987 [CrossRef]
    [Google Scholar]
  19. Kumar S., Tamura K., Nei M. 1993; mega: molecular evolutionary analysis software for microcomputers. CABIOS 10:189–191
    [Google Scholar]
  20. Lomholt H. 1995; Evidence of recombination and an antigenically diverse immunoglobulin A1 protease among strains of Streptococcus pneumoniae. Infect Immun 63:4238–4243
    [Google Scholar]
  21. McDougal L. K., Facklam R., Reeves M., Hunter S., Swenson J. M., Hill B. C., Tenover F. C. 1992; Analysis of multiply antimicrobial-resistant isolates of Streptococcus pneumoniae from the United States. Antimicrob Agents Chemother 36:2176–2177 [CrossRef]
    [Google Scholar]
  22. Maiden M. C. J., Bygraves J. A., Feil E.10 other authors 1998; Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic micro-organisms. Proc Natl Acad Sci USA 95:3140–3145 [CrossRef]
    [Google Scholar]
  23. Maynard Smith J. 1992; Analysing the mosaic structure of genes. J Mol Evol 34:126–129
    [Google Scholar]
  24. Maynard Smith J., Smith N. H., O’Rourke M., Spratt B. G. 1993; How clonal are bacteria?. Proc Natl Acad Sci USA 90:4384–4388 [CrossRef]
    [Google Scholar]
  25. Muñoz R., Coffey T. J., Dowson C. G., Laible G., Casal J., Hakenbeck R., Jacobs M., Musser J. M., Spratt B. G., Tomasz A. 1991; Interncontinental spread of a multiresistant clone of serotype 23F Streptococcus pneumoniae. J Infect Dis 164:302–306 [CrossRef]
    [Google Scholar]
  26. Musser J. M. 1996; Molecular population genetic analysis of emerged bacterial pathogens: selected insights. Emerg Infect Dis 2:1–17 [CrossRef]
    [Google Scholar]
  27. Nesin M., Ramirez M., Tomasz A. 1998; Capsular transformation of a multidrug-resistant Streptococcus pneumoniae in vivo. J Infect Dis 177:707–713 [CrossRef]
    [Google Scholar]
  28. O’Rourke M., Stevens E. 1993; Genetic structure of Neisseria gonorrhoeae populations: a non-clonal pathogen. J Gen Microbiol 139:2603–2611 [CrossRef]
    [Google Scholar]
  29. Paul J. 1997; HIV and pneumococcal infection in Africa. Trans R Soc Trop Med Hyg 91:632–637 [CrossRef]
    [Google Scholar]
  30. Paul J., Bates J., Kimari J., Gilks C. 1996; Serotypes and antibiotic susceptibilities of Streptococcus pneumoniae in Nairobi, Kenya. J Infect 32:139–142 [CrossRef]
    [Google Scholar]
  31. Pons J.-L., Mandement M.-N., Martin E., Lemort C., Nouvellon M., Mallet E., Lemeland J.-F. 1996; Clonal and temporal patterns of nasopharyngeal penicillin-susceptible and penicillin-resistant Streptococcus pneumoniae strains in children attending a day care center. J Clin Microbiol 34:3218–3222
    [Google Scholar]
  32. SAS Institute 1990 SAS User’s Guide, Version 6 Cary, NC: SAS Institute.;
    [Google Scholar]
  33. Sawyer S. 1989; Statistical tests for detecting gene conversation. Mol Biol Evol 6:526–538
    [Google Scholar]
  34. Scott J. A. G., Hall A. J., Dagan R.12 other authors 1996; Serogroup-specific epidemiology of Streptococcus pneumoniae – associations with age, sex, and geography in 7000 episodes of invasive disease. Clin Infect Dis 22:973–981 [CrossRef]
    [Google Scholar]
  35. Sibold C., Wang J., Henrichsen J., Hakenbeck R. 1992; Genetic relationships of penicillin-susceptible and -resistant Streptococcus pneumoniae strains isolated on different continents. Infect Immun 60:4119–4126
    [Google Scholar]
  36. Souza V., Nguyen T. T., Hudson R. R., Piñero D., Lenski R. E. 1992; Hierarchical analysis of linkage disequilibrium in Rhizobium populations: evidence for sex?. Proc Natl Acad Sci USA 89:8389–8393 [CrossRef]
    [Google Scholar]
  37. Speller D. C. E., Johnson A. P., Cookson B. D., Waight P., George R. C. 1996; PHLS surveillance of antibiotic resistance, England and Wales: emerging resistance in Streptococcus pneumoniae. Emerg Infect Dis 2:57–58 [CrossRef]
    [Google Scholar]
  38. Sung R. Y. T., Ling J. M., Fung S. M., Oppenheimer S. J., Crook D. W., Lau J. T. F., Cheng A. F. B. 1995; Carriage of Haemophilus influenzae and Streptococcus pneumoniae in healthy Chinese and Vietnamese children in Hong Kong. Acta Paediatr 84:1262–1267 [CrossRef]
    [Google Scholar]
  39. Versalovic J., Kapur V., Mason E. O., Shah V., Keouth T., Lupski J. R., Musser J. M. 1993; Penicillin-resistant Streptococcus pneumoniae strains recovered in Houston: identification and molecular characterisation of multiple clones. J Infect Dis 167:850–856 [CrossRef]
    [Google Scholar]
  40. Weiser J. N., Austrian R., Screenivasan P. K., Masure H. R. 1994; Phase variation in pneumococcal opacity – relationship between colonial morphology and nasopharyngeal colonization. Infect Immun 62:2582–2589
    [Google Scholar]
  41. Whatmore A. M., King S. J., Doherty N., Sturgeon D., Chanter N., Dowson C. G. 1999; Molecular characterisation of equine isolates of Streptococcus pneumoniae: natural disruption of genes encoding the virulence factors pneumolysin and autolysin. Infect Immun 67:2776–2782
    [Google Scholar]
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