1887

Abstract

Two genes, and , with nearly identical nucleotide sequences were cloned from A3(2). The deduced amino acid sequences of the product of these two genes showed high similarity to BcpA2 of and other biotin-containing proteins from different organisms assumed to be the α subunit of a propionyl-CoA carboxylase. A gene, , encoding the carboxyl transferase subunit of this enzyme complex was also characterized. Strains disrupted in did not show any change in acetyl- or propionyl-CoA carboxylase activity, whilst cell-free extracts of a mutant strain contained a reduced level of propionyl-CoA carboxylase. No mutants in could be isolated, suggesting that the gene may be essential. Heterologous expression of , and in and reconstitution of enzyme activity confirmed that PccB is the β subunit of a propionyl-CoA carboxylase and that either AccA1 or AccA2 could act as the α component of this enzyme complex. The fact that mutants appear to be inviable suggests that this gene encodes a biotinylated protein that might be shared with other carboxyl transferases essential for the growth of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-145-11-3109
1999-11-01
2020-07-11
Loading full text...

Full text loading...

/deliver/fulltext/micro/145/11/1453109a.html?itemId=/content/journal/micro/10.1099/00221287-145-11-3109&mimeType=html&fmt=ahah

References

  1. Barker D., Campbell A.. 1981; The birA gene of Escherichia coli encodes a biotin holoenzyme synthetase. J Mol Biol146:469–492[CrossRef]
    [Google Scholar]
  2. Bartolomé, B., Jubete Y., Martı́nez, E., de la Cruz F.. 1991; Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene102:75–78[CrossRef]
    [Google Scholar]
  3. Behal V., Jechova V., Vanek Z., Hostalek Z.. 1977; Alternative pathways of malonyl-CoA formation in Streptomyces aureofaciens. Phytochemistry16:347–350[CrossRef]
    [Google Scholar]
  4. Best E., Knauf V.. 1993; Organization and nucleotide sequences of the genes encoding the biotin carboxyl carrier protein and biotin carboxylase protein of Pseudomonas aeruginosa acetyl coenzyme A carboxylase. J Bacteriol175:6881–6889
    [Google Scholar]
  5. Bierman M., Logan R., O’Brien K., Seno E. T., Nagaranja Rao R., Schoner B. E.. 1992; Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene116:43–49[CrossRef]
    [Google Scholar]
  6. Birch A., Leiser A., Robinson J.. 1993; Cloning, sequencing, and expression of the gene encoding methylmalonyl-CoA mutase from Streptomyces cinnamonensis. J Bacteriol175:3511–3519
    [Google Scholar]
  7. Bloch K., Vance D.. 1977; Control mechanisms in the synthesis of saturated fatty acids. Annu Rev Biochem46:263–298[CrossRef]
    [Google Scholar]
  8. Bradford M.. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem72:248–254[CrossRef]
    [Google Scholar]
  9. Bramwell H., Nimmo H. G., Hunter I. S., Coggins J. R.. 1993; Phosphoenolpyruvate carboxylase from Streptomyces coelicolor A3(2): purification of the enzyme, cloning of the ppc gene and over-expression of the protein in a streptomycete. Biochem J293:131–136
    [Google Scholar]
  10. Bramwell H., Hunter I. S., Coggins J. R., Nimmo H. G.. 1996; Propionyl-CoA carboxylase from Streptomyces coelicolor A3(2): cloning of the gene encoding the biotin-containing subunit. Microbiology142:649–655[CrossRef]
    [Google Scholar]
  11. Browner M., Taroni F., Stzul E., Rosenberg L.. 1989; Sequence analysis, biogenesis and mitochondrial import of the alpha subunit of rat liver propionyl-CoA carboxylase. J Biol Chem264:1280–1285
    [Google Scholar]
  12. Burnette W. N.. 1981; ‘‘Western blotting’’: electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem112:195–203[CrossRef]
    [Google Scholar]
  13. Cole S. T., Brosch R., Parkhill J..39 other authors 1998; Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature393:537–544[CrossRef]
    [Google Scholar]
  14. Cronan J. E. Jr. 1990; Biotination of proteins in vivo. J Biol Chem265:10327–10333
    [Google Scholar]
  15. Donadio S., Staver M., Katz L.. 1996; Erythromycin production in Saccharopolyspora erythraea does not require a functional propionyl-CoA carboxylase. Mol Microbiol19:977–984[CrossRef]
    [Google Scholar]
  16. Erfle J. D.. 1973; Acetyl-CoA and propionyl-CoA carboxylation by Mycobacterium phlei. Partial purification and some properties of the enzyme. Biochim Biophys Acta316:143–155[CrossRef]
    [Google Scholar]
  17. Floriano B., Bibb M. J.. 1996; afsR is a pleiotropic but conditionally required regulatory gene for antibiotic production in Streptomyces coelicolor A3(2). Mol Microbiol21:385–396[CrossRef]
    [Google Scholar]
  18. Gorst-Allman C. P., Rudd B. A. M., Chang C. J., Floss H. G.. 1981; Biosynthesis of actinorhodin. Point of dimerization. J Org Chem46:455–456[CrossRef]
    [Google Scholar]
  19. Gottschalk G.. 1986; Bacterial metabolism, 2nd edn. New York: Springer;
    [Google Scholar]
  20. Hanahan D.. 1983; Studies of transformation of Escherichia coli with plasmids. J Mol Biol166:557–580[CrossRef]
    [Google Scholar]
  21. Harder M. E., Beacham I., Cronan J. Jr, Beachan K., Honegger J., Silbert D.. 1972; Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties. Proc Natl Acad Sci USA69:3105–3109[CrossRef]
    [Google Scholar]
  22. Harwood J. L.. 1988; Fatty acid metabolism. Annu Rev Plant Physiol39:101–138[CrossRef]
    [Google Scholar]
  23. Henrikson K. P., Allen S. H. G.. 1979; Purification and subunit structure of propionyl coenzyme A carboxylase of Mycobacterium smegmatis. J Biol Chem254:5888–5891
    [Google Scholar]
  24. Hopwood D. A., Sherman D. H.. 1990; Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Annu Rev Genet24:37–66[CrossRef]
    [Google Scholar]
  25. Hopwood D. A., Bibb M. J., Chater K. F..7 other authors 1985; Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  26. Hopwood D., Chater K., Bibb M. J.. 1994; Genetics of antibiotic production in Streptomyces coelicolor A3(2), a model streptomycete. In Genetics and Biochemistry of Antibiotic Production pp.65–102Edited by Vining L. C., Studdard C.. Wenheim: Butterworth-Heinemann;
    [Google Scholar]
  27. Hunaiti A. R., Kolattukudy P. E.. 1982; Isolation and characterisation of an acyl-coenzyme A carboxylase from an erythromycin-producing Streptomyces erythyreus. Arch Biochem Biophys216:362–371[CrossRef]
    [Google Scholar]
  28. Janssen G. R., Bibb M. J.. 1993; Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene124:133–134[CrossRef]
    [Google Scholar]
  29. Kao C., Luo G., Katz L., Cane D., Khosla C.. 1994; Engineered biosynthesis of a complete macrolactone in a heterologous host. J Am Chem Soc116:11612–11613[CrossRef]
    [Google Scholar]
  30. Katz L., Donadio S.. 1993; Polyketide synthesis: prospects for hybrid antibiotics. Annu Rev Microbiol47:875–912[CrossRef]
    [Google Scholar]
  31. Kondo H., Shiratsuchi K., Yoshimoto T., Masuda T., Kitazono A., Tsuru D., Anai M., Sekiguchi M., Tanabe T.. 1991; Acetyl-CoA carboxylase from Escherichia coli: gene organization and nucleotide sequence of the biotin carboxylase subunit. Proc Natl Acad Sci USA88:9730–9733[CrossRef]
    [Google Scholar]
  32. Laakel M., Lebrihi A., Khaoua S., Schneider F., Lefebvre G., Germain P.. 1994; A link between primary and secondary metabolism: malonyl-CoA formation in Streptomyces ambofaciens growing on ammonium ions or valine. Microbiology140:1451–1456[CrossRef]
    [Google Scholar]
  33. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685[CrossRef]
    [Google Scholar]
  34. Li S. J., Cronan J. E. Jr. 1992; The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase. J Biol Chem267:16841–16847
    [Google Scholar]
  35. MacNeil D. J., Gewain K. M., Ruby C. L., Dezeny G., Gibbons P. H., MacNeil T.. 1992; Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene111:61–68[CrossRef]
    [Google Scholar]
  36. Martı́n, J. F., Liras P.. 1989; Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites. Annu Rev Microbiol43:173–206[CrossRef]
    [Google Scholar]
  37. Nikolau B. J., Wurtele E. S., Stumpf P. K.. 1985; Acetyl-coenzyme A carboxylase in maize leaves. Anal Biochem149:448–453[CrossRef]
    [Google Scholar]
  38. Norman E., De Smet K. A. L., Stoker N. G., Ratledge C., Wheeler P. R., Dale J. W.. 1994; Lipid synthesis in Mycobacteria: characterization of the biotin carboxyl carrier protein genes from Mycobacterium leprae and Mycobacterium tuberculosis. J Bacteriol176:2525–2531
    [Google Scholar]
  39. Paget M. S. B., Chamberlin L., Atrih A., Foster S. J., Buttner M. J.. 1999; Evidence that the extracytoplasmic function sigma factor σE is required for normal cell wall structure in Streptomyces coelicolor A3(2). J. Bacteriol181:204–211
    [Google Scholar]
  40. Polakis S., Guchhait R., Lane M.. 1972; On the possible involvement of a carbonyl phosphate group intermediate in the adenosine triphosphate-dependent carboxylation of biotin. J Biol Chem247:1335–1337
    [Google Scholar]
  41. Redenbach M., Kieser H. M., Denapaite D., Eichner A., Cullum J., Kinashi H., Hopwood D.. 1996; A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol Microbiol21:77–95[CrossRef]
    [Google Scholar]
  42. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  43. Sanger F., Nicklen S., Coulson A. R.. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA74:5463–5467[CrossRef]
    [Google Scholar]
  44. Studier F. W., Moffatt B. A.. 1986; Use of the bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol189:113–130[CrossRef]
    [Google Scholar]
  45. Wasserman H., Rodgers G., Keith D.. 1976; Undecylprodigiosin. Tetrahedron32:1851–1854[CrossRef]
    [Google Scholar]
  46. Wright F., Bibb M. J.. 1992; Codon usage in the G+C-rich Streptomyces genome. Gene113:55–65[CrossRef]
    [Google Scholar]
  47. Zerbe-Burkhardt K., Ratnatilleke A., Phillipon N., Birch A., Leiser A., Vrijbloed J., Hess D., Hunziker P., Robinson J.. 1998; Cloning, sequencing, expression, and insertional inactivation of the gene for the large subunit of the coenzyme B12-dependent isobutyryl-CoA mutase from Streptomyces cinnamonensis. J Biol Chem273:6508–6517[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-145-11-3109
Loading
/content/journal/micro/10.1099/00221287-145-11-3109
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error