1887

Abstract

The authors previously identified large plasmids in Mycobacterium xenopi, M. branderi and M. celatum which appeared to have a linear topology. This study has confirmed the presence of such linear plasmids in mycobacteria, including M. avium, and demonstrated that the ends of these replicons are covalently bound with protein(s), suggesting an invertron-like structure. The termini of one 25 kb plasmid, designated pCLP, from M. celatum were cloned and the first 500 bp of each terminus were sequenced. The termini of this plasmid show the characteristic features of invertrons with terminal inverted repeats of 45 bp (with imperfect matches) and several palindromic sequences. Moreover, similarity existed in the structure and terminal nucleotide sequence of pCLP and the termini of linear replicons of Streptomyces and Rhodococcus species, indicating a conservation of these linear extrachromosomal elements within the Actinomycetales.

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1998-07-01
2022-05-28
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References

  1. Berdy J. 1980; Recent advances in and prospects for antibiotic research. . Process Biochem 15:28–35
    [Google Scholar]
  2. Bimboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. . Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  3. Chang P.C., Kim E.S., Cohen S.N. 1996; Streptomyces linear plasmids that contain a phage-like, centrally located, replication origin.. Mol Microbiol 22:789–800
    [Google Scholar]
  4. Chardon-Loriaux I., Charpentier M., Percheron F. 1986; Isolation and characterization of a linear plasmid from Streptomyces rimosus. . FEMS Microbiol Lett 35:151–155
    [Google Scholar]
  5. Chen C.W. 1996; Complications and implications of linear bacterial chromosomes.. Trends Genet 12:192–196
    [Google Scholar]
  6. Chen C.W., Yu T.W., Lin Y.S., Kieser H.M., Hopwood D.A. 1993; . The conjugative plasmid SLP2 of Streptomyces lividans is a 50 kb linear molecule.. Mol Microbiol 7:925–932
    [Google Scholar]
  7. Chou S.H., Zhu L, Reid B.R. 1997; Sheared purine pairing in biology.. J Mol Biol 267:1055–1067
    [Google Scholar]
  8. Crespi M., Messens E., Caplan A.B., van Montagu M., Desomer J. 1992; Fasciation induction by the phytopathogen Rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene.. EMBO J 11:795–804
    [Google Scholar]
  9. Crespi M., Vereecke D., Temmerman W., vanMontagu M., Desomer J. 1994; The fas operon of Rhodococcus fascians encodes new genes required for efficient fasciation of host plants.. J Bacteriol 176:2492–2501
    [Google Scholar]
  10. Dabrock B., Kebeler M., Averhoff B., Gottschalk G. 1994; Identification and characterization of a transmissible linear plasmid from Rhodococcus erythropolis BD2 that encodes isopropylbenzene and trichloroethene catabolism.. Appl Environ Microbiol 60:853–860
    [Google Scholar]
  11. DeMot R., Nagy I., DeSchrijver A., Pattanapipitpaisal P., Schoofs G., Vanderleyden J. 1997; Structural analysis of the 6 kb cryptic plasmid pFAJ2600 from Rhodococcus erythropolis NI86/21 and construction of Escherichia coli-Rhodococcus shuttle vectors.. Microbiology 143:3137–3147
    [Google Scholar]
  12. Evans M., Kaczmarek F.S., Stutzman-Engwall K., Dyson P. 1994; . Characterization of a Streptomyces lividans-type site- specific DNA modification system in the avermectin producer Streptomyces avermitilis permits investigation of two novel giant linear plasmids, pSAl and pSA2.. Microbiology 140:1367–1371
    [Google Scholar]
  13. Finnerty W.R. 1992; The biology and genetics of the genus Rhodococcus. . Annu Rev Microbiol 46:193–218
    [Google Scholar]
  14. Gavigan J.A., Ainsa J.A., Pérez E., Otal I., Martin C. 1997; Isolation by genetic labeling of a new mycobacterial plasmid, pJAZ38, from Mycobacterium fortuitum. . J Bacteriol 179:4115–4122
    [Google Scholar]
  15. Gravius B., Glocker D., Pigac J., Pandza K., Hranueli D., Cullum J. 1994; The 387 kb linear plasmid pPZGIOl of Streptomyces rimosus and its interactions with the chromosome.. Microbiology 140:2271–2277
    [Google Scholar]
  16. Hanafusa T., Kinashi H. 1992; The structure of an integrated copy of the giant linear plasmid SCP1 in the chromosome of Streptomyces coelicolor 2612.. Mol Gen Genet 231:363–368
    [Google Scholar]
  17. Hayakawa T., Tanaka T., Sakaguchi K., Otake N., Yonehara H. 1979; A linear plasmid-like DNA in Streptomyces sp. producing lankacidin group antibiotics.. J Gen Appl Microbiol 25:255–260
    [Google Scholar]
  18. Hinnebusch J., Tilly K. 1993; Linear plasmids and chromosomes in bacteria.. Mol Microbiol 10:917–922
    [Google Scholar]
  19. Hinnebusch J., Bergström S., Barbour A.G. 1990; Cloning and sequence analysis of linear plasmid telomeres of the bacterium Borrelia burgdorferi. . Mol Microbiol 4:811–820
    [Google Scholar]
  20. Hirochika H., Sakaguchi K. 1982; Analysis of linear plasmids isolated from Streptomyces: association of protein with the ends of the plasmid DNA.. Plasmid 7:59–65
    [Google Scholar]
  21. Hirochika H., Nakamura K., Sakaguchi K. 1984; A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.. EMBO J 3:761–766
    [Google Scholar]
  22. Kalkus J., Reh M., Schlegel H.G. 1990; Hydrogen autotrophy of Nocardia opaca strains is encoded by linear megaplasmids.. J Gen Microbiol 136:1145–1151
    [Google Scholar]
  23. Kalkus J., Dörrie G, Fischer D., Reh M., Schlegel H.G. 1993; The giant linear plasmid pHG207 from Rhodococcus sp. encoding hydrogen autotrophy: characterization of the plasmid and its termini.. J Gen Microbiol 139:2055–2065
    [Google Scholar]
  24. Kato K., Ohtsuki K., Mitsuda H., Yomo T., Negoro S., Urabe I. 1994; Insertion sequence IS6100 on plasmid pOAD2, which degrades nylon oligomers.. J Bacteriol 176:1197–1200
    [Google Scholar]
  25. Keen C. L., Mendelovitz S., Cohen G., Aharonowitz Y., Roy K. L. 1994; Insertion sequence IS6100 on plasmid pOAD2, which degrades nylon oligomers.. J Bacteriol 176:1197–1200
    [Google Scholar]
  26. Kebeler M., Dabbs E.R., Averhoff B., Gottschalk G. 1996; Studies on the isopropylbenzene 2,3-dioxygenase and the 3- isopropylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of Rhodococcus erythropolis BD2.. Microbiology 142:3241–3251
    [Google Scholar]
  27. Keen C.L, Mendelovitz S., Cohen G., Aharonowitz Y., Roy K.L. 1988; Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus. . Mol Gen Genet 212:172–176
    [Google Scholar]
  28. Kinashi H. 1994; Linear plasmids from Actinomycetes.. Actino- mycetologica 8:87–96
    [Google Scholar]
  29. Kinashi H., Shimaji-Murayama M. 1991; Physical characterization of SCP1, a giant linear plasmid from Streptomyces coelicolor. . J Bacteriol 173:1523–1529
    [Google Scholar]
  30. Kinashi H., Shimaji M., Sakai A. 1987; Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes.. Nature 328:454–456
    [Google Scholar]
  31. Kinashi H., Shimaji-Murayama M., Hanafusa T. 1992; Integration of SCP1, a giant linear plasmid, into the Streptomyces coelicolor chromosome.. Gene 115:35–41
    [Google Scholar]
  32. Kosono S., Maeda M., Fuji F., Arai H., Kudo T. 1997; Three of the seven bphC genes of Rhodoccoccus erythropolis TA421, isolated from a termite ecosystem, are located on an indigenous plasmid associated with biphenyl degradation.. Appl Environ Microbiol 63:3282–3285
    [Google Scholar]
  33. Kulakov L.A., Larkin M.J., Kulakova A.N. 1997; Cryptic plasmid pKA22 isolated from the naphthalene degrading derivative of Rhodococcus rhodochrous NCIMB 13064.. Plasmid 38:61–69
    [Google Scholar]
  34. Lazrak R., Clavel-Sérés S., David H.L., Rouiland-Dussoix D. 1990; Conjugative transfer of a shuttle plasmid for Escherichia coli to Mycobacterium smegmatis. . FEMS Microbiol Lett 69:135–138
    [Google Scholar]
  35. Leblond P., Francou F.X., Simonet J.M., Decaris B. 1990; Pulsed-field gel electrophoresis analysis of the genome of Streptomyces ambofaciens strains.. FEMS Microbiol Lett 72:79–88
    [Google Scholar]
  36. Leblond P., Fischer G., Francou F.X., Berger F., Guérineau M., Decaris B. 1996; The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA.. Mol Microbiol 19:261–271
    [Google Scholar]
  37. Lin Y.S., Kieser H.M., Hopwood D.A., Chen C.W. 1993; The chromosomal DNA of Streptomyces lividans 66 is linear.. Mol Microbiol 10:923–933
    [Google Scholar]
  38. Philipp W.J., Poulet S., Eiglmeier K. 7 other authors 1996; An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae. . Proc Natl Acad Sci USA 933132–3137
    [Google Scholar]
  39. Picardeau M., Vincent V. 1997; Characterization of large linear plasmids in mycobacteria.. J Bacteriol 179:2753–2756
    [Google Scholar]
  40. Picardeau M., Varnerot A., Rauzier J., Gicquel B., Vincent V. 1996; Mycobacterium xenopi IS1395, a novel insertion sequence expanding the IS256 family.. Microbiology 142:2453–2461
    [Google Scholar]
  41. Picardeau M., Bull T.J., Vincent V. 1997; Identification and characterization of IS-like elements in Mycobacterium gordonae. . FEMS Microbiol Lett 154:95–102
    [Google Scholar]
  42. Sakaguchi K. 1990; Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adeno-type viruses.. Microbiol Rev 54:66–74
    [Google Scholar]
  43. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  44. Stackebrandt E., Rainey F.A., Ward-Rainey N.L. 1997; Proposal for a new hierarchic classification system, Actino- bacteria classis nov.. Int J Syst Bacteriol 47:479–491
    [Google Scholar]
  45. Wu X., Roy K.L. 1993; Complete nucleotide sequence of a linear plasmid from Streptomyces clavuligerus and characterization of its RNA transcripts.. J Bacteriol 175:37–52
    [Google Scholar]
  46. Zotchev S.B., Soldatova L.I., Orekhov A.V., Schrempf H. 1992; Characterization of a linear extrachromosomal DNA element (pBLl) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans. . Res Microbiol 143:839–845
    [Google Scholar]
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