1887

Abstract

Streptomycetes differ from other prokaryotic organisms in their mycelial life cycle and in possessing a large, linear, GC-rich chromosome. To deduce structural features of the origin of chromosomal replication, the sequences of three species ( and ) were compared. In , the region contains 19 DnaA boxes whose location, orientation and spacing are conserved. The consensus sequence of the DnaA box identified within is (/C)(/C)(/A/C) (preferred bases underlined). The interactions of DnaA with DNA fragments containing single, two or three DnaA boxes were studied using surface plasmon resonance. The dissociation constant ( ) for specific binding of individual DnaA boxes varied between 12 and 78 nM. does not contain the three AT-rich 13-mer direct repeats present in the 5′ part of the region. However, short AT-rich sequences are distributed among the DnaA boxes of Repeated attempts to unwind have been unsuccessful. It remains to be elucidated whether DnaA interacts with putative accessory proteins which help in unwinding Streptomyces oriC.

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1998-05-01
2021-05-08
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