1887

Abstract

A cDNA encoding a 37 kDa protein was isolated from an expression library using antibodies raised against mycelial cell walls from The 37 kDa protein has over 60% sequence identity with the 37 kDa laminin-binding protein (LBP) from humans and over 80% identity with the Yst proteins of . The protein was named CaYst1. It was found in membrane and ribosome fractions but surprisingly, was not found in cell walls. Unlike the human LBP, CaYst1p does not bind laminin. These data indicate that CaYst1p is not a cell-surface receptor for laminin as has been proposed for the human LBP. Instead, like the Yst proteins, it appears to be a ribosomal protein. This conclusion is supported by the finding that -cDNA complements the lethal phenotype linked to the disruption of both genes in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-4-839
1998-04-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/4/mic-144-4-839.html?itemId=/content/journal/micro/10.1099/00221287-144-4-839&mimeType=html&fmt=ahah

References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. (1990); Basic local alignment search tool.. J 215:403–410 [View Article]
    [Google Scholar]
  2. Auth D., Brawerman G. (1992); A 33-kDa polypeptide with homology to the laminin receptor: component of translation machinery.. Proc Natl Acad Sci USA 89:4368–4372 [View Article]
    [Google Scholar]
  3. Bouchara J.P., Tronchin G., Annaix V., Robert R., Senet J.M. (1990); Laminin receptors on Candida albicans germ tubes.. Infection and Immunity 58:48–54 [View Article]
    [Google Scholar]
  4. Calderone R., Braun P.C. (1991); Adherence and receptor relationships of Candida albicans.. Microbiological Reviews 55:1–20 [View Article]
    [Google Scholar]
  5. Casanova M., Gil M.L., Cardefioso L., Martinez J.P., Sentandreu R. (1989); Identification of wall-specific antigens synthesized during germ tube formation by Candida albicans.. Infection and Immunity 57:262–271 [View Article]
    [Google Scholar]
  6. Casanova M., Lopez-Ribot J.L., Martinez i P, Sentandreu R. (1992); Characterization of cell wall proteins from yeast and mycelial cells of Candida albicans by labeling with biotin: comparison with other techniques.. Infection and Immunity 60:4898–4906 [View Article]
    [Google Scholar]
  7. G, Sobel E.M. (1991); Functional domains of the 67-kDa laminin receptor precursor.. Journal of Biological Chemistry 266:20440–20446 [View Article]
    [Google Scholar]
  8. Demianova M., Formosa T.G., Ellis R.S. (1996); Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40S ribosomal subunit.. Journal of Biological Chemistry 271:11383–11391 [View Article]
    [Google Scholar]
  9. Elorza M.V., Rico H., Gozalbo D., Sentandreu R. (1983); Cell wall composition and protoplast regeneration in Candida albicans.. Antonie Leeuwenhoek 49:457–469
    [Google Scholar]
  10. Elorza M.V., Marcilla A., Sentandreu R. (1988); Wall manno- proteins of the yeast and mycelial cells of Candida albicans: nature of the glycosidic bonds and polydispersity of their mannan moieties.. ] Gen Microbiol 134:2393–2403
    [Google Scholar]
  11. Eroles P., Sentandreu M., Elorza M.V., Sentandreu R. (1995); Cloning of a DNA fragment encoding part of a 70 kDa heat shock protein of Candida albicans. Fems Microbiology Letters 12895–100
    [Google Scholar]
  12. Eroles P., Sentandreu M., Elorza M.V., Sentandreu R. (1997); The highly immunogenic enolase and Hsp70p are adventitious Candida albicans cell wall proteins. Microbiology 143313–320
    [Google Scholar]
  13. Garcfa-Hern£ndez M., Davis E., Staswick P.E. (1994); Arabidopsis p40 homologue: a novel acidic protein associated with the 40S subunit of ribosomes. Journal of Biological Chemistry,269,20744–20749
    [Google Scholar]
  14. Glee P.M., Masuoka J., Ozier W.T., Hazen K.C. (1996); Presence of multiple laminin- and fibronectin-binding proteins in cell wall extracts of Candida albicans: influence of dialysis. ] Med Vet Mycol 3457–61
    [Google Scholar]
  15. Gozalbo D., Elorza M.V., Sanjudn R., Marcilla A., Valentin E., Sentandreu R. (1994); Critical steps in fungal cell wall synthesis: strategies for their inhibition.. Pharmacol Ther 60:337–345 [View Article]
    [Google Scholar]
  16. Hanahan D. (1983); Studies on transformation of Escherichia coli with plasmids.. } Mol Biol 166:557–580 [View Article]
    [Google Scholar]
  17. Hoffman C.S., Winston F. (1987); A ten minute DNA preparation from yeast efficiently releases autonomous plasmid for transformation of Escherichia coli.. Gene 57:267–272 [View Article]
    [Google Scholar]
  18. Ito H., Fukuda Y., Murata K., Kimura A. (1983); Transformation of intact yeast cells treated with alkali cations.. Journal of Bacteriology 153:163–168 [View Article]
    [Google Scholar]
  19. Keppel E., Schaller H.C. (1991); A 33 kDa protein with sequence homology to the ‘laminin binding protein’ is associated with cytoskeleton in hydra and in mammalian cells.. J Cell Science 100:789–797
    [Google Scholar]
  20. Kyte J., Doolittle R.F. (1982); A simple method for displaying the hydropathic character of a protein.. Journal of Molecular Biology 157:105–132 [View Article]
    [Google Scholar]
  21. Laemmli U.K. (1970); Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685 [View Article]
    [Google Scholar]
  22. Lee K.L., Buckley M.R., Campbell C. (1975); An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans.. Sabouraudia-journal of Medical and Veterinary Mycology 13:148–153 [View Article]
    [Google Scholar]
  23. L6pez-Ribot J.L., Casanova M., Martinez J.P., Sentandreu R. (1991); Characterization of cell wall proteins of yeast and hydrophobic mycelial cells of Candida albicans.. Infection and Immunity 59:2324–2332 [View Article]
    [Google Scholar]
  24. L6pez-Ribot J.L., Casanova M., Monteagudo C., Sepulveda P., Martinez J.P. (1994); Evidence for the presence of a high- affinity laminin receptor-like molecule in the surface of Candida albicans yeast cells.. Infection and Immunity 62:742–746 [View Article]
    [Google Scholar]
  25. Ldpez-Ribot J.L., Monteagudo C., Sepulveda P., Casanova M., Martinez J.P., Chaffin W.L. (1996); Expression of the fibrinogen binding mannoprotein and the laminin receptor of Candida albicans in vitro and in infected tissues.. Fems Microbiology Letters 142:117–122 [View Article]
    [Google Scholar]
  26. Mdlhinney RAJ. (1990); The fats of life: the importance and function of protein acylation.. Trends in Biochemical Sciences 15:387–391 [View Article]
    [Google Scholar]
  27. Marcilla A., Elorza M.V., Mormeneo S., Rico H., Sentandreu R. (1991); Candida albicans mycelial wall structure: supra- molecular complexes released by Zymolyase, chitinase and /?- mercaptoethanol.. Archives of Microbiology 155:312–319 [View Article]
    [Google Scholar]
  28. Mecham R.P. (1991); Receptors for laminin on mammalian cells.. Faseb Journal 5:2538–2546 [View Article]
    [Google Scholar]
  29. Moukadiri I., Armero J., Abad A., Sentandreu R., Zueco J. (1997); Identification of a mannoprotein present in the inner layer of the cell wall of Saccharomyces cerevisiae.. Journal of Bacteriology 179:2154–2162 [View Article]
    [Google Scholar]
  30. Narasimhan S., Armstrong MYK, Rhee K., Edman J.C., Richards F.F., Spicer E. (1994); Gene for an extracellular matrix receptor protein from Pneumocystis carinii.. Proc Natl Acad Sci USA 91:7440–7444 [View Article]
    [Google Scholar]
  31. Odds F.C. 1988 Candida and Candidosis. A Review and Bibliography London:: Bailliere Tindall;42–59
    [Google Scholar]
  32. Ram6n A.M., Gil M.R., Burgal M., Sentandreu R., Valentfn R. (1996); A novel cell wall protein specific to the mycelial form of Yarrowia lipolytica.. Yeast 12:1535–1548 [View Article]
    [Google Scholar]
  33. Rambn A.M., Valentfn E., Maicas S., Sentandreu R. (1997); Expression of YWP1, a gene that codifies a specific Yarrowia lipolytica mycelial cell wall protein, in Saccharomyces cerevisiae.. Fungal Genetics and Biology 22:77–83 [View Article]
    [Google Scholar]
  34. Randez-Gil F., Prieto J.A., Murcia A., Sanz P. (1995); Construction of baker’s yeast strains that secrete Aspergillus oryzae alpha-amylase and their use in bread making. Journal of Cereal Science 21185–193
    [Google Scholar]
  35. Rao MJK, Argo P. (1986); A conformational preference parameter to predict helices in integral membrane proteins.. Biochimica Et Biophysica Acta 869:197–214 [View Article]
    [Google Scholar]
  36. Rau£ H.A., Mager W.H. 1991 8< Planta, R, Structural and functional analysis of the yeast ribosomal proteins.. In Guide to Yeast Genetics and Molecular Biology,453—477 Edited by Guthrie C., Fink G. R. San Diego:: Academic Press;
    [Google Scholar]
  37. Rosenthal E.T., Worderman L. (1995); A protein similar to the 67 kDa laminin binding protein and p40 is probably a component of the translational machinery in Urechis caupo oocytes and embryos.. Journal of Cell Science 108:245–256
    [Google Scholar]
  38. Sambrook J., Fritsch E.F., Maniatis T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. edition, Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  39. Sanger F., Nicklen S., Coulson A.R. (1977); DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467 [View Article]
    [Google Scholar]
  40. Sentandreu M., Elorza M.V., Valentfn E., Sentandreu R., Gozalbo D. (1995); Cloning of cDNAs coding for Candida albicans cell surface proteins.. Journal of Medical and Veterinary Mycology 33:105–111 [View Article]
    [Google Scholar]
  41. Sentandreu R., Elorza M.V., Mormeneo S., Sanjudn R., Iranzo M. 1993 Possible roles of mannoproteins in the construction of Candida albicans cell wall.. In: Bossche HVanden, Odds F., Kerndge D. eds. Dimorphic Fungi in Biology and Medicine,169–175 New York:: Plenum;
    [Google Scholar]
  42. Sherman F. 1991 Getting started with yeast.. In: Guthrie C., Fink G.R. eds. Guide to Yeast Genetics and Molecular Biology San Diego:: Academic Press;3—21
    [Google Scholar]
  43. Towbin H., Staehelin T., Gordon J. (1979); Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proc Natl Acad Sci USA 76:4350–4354 [View Article]
    [Google Scholar]
  44. Wool I.G. (1996); Extraribosomal functions of ribosomal proteins.. Trends Biol Sci 21:164–165 [View Article]
    [Google Scholar]
  45. L.&Warner WoolfordJ, J. R. (1991) The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis and Energetics Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  46. M. J., Chen H.S., Lee C., Steele G.D., Chen L.B. (1988); Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full- length cDNA encoding the protein.. Proc Natl Acad Sci USA 85:6394–6398 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-4-839
Loading
/content/journal/micro/10.1099/00221287-144-4-839
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error