1887

Abstract

The gene region of was cloned and sequenced. A tRNA gene, seven ORFs and three DnaA box clusters were identified. The order of the genes and intergene regions found was tRNA--DnaA box cluster 3--DnaA box cluster 2--DnaA box cluster 1-. Five ORFs are homologous to known bacterial genes. The tRNA gene and , also called , have already been described in pneumococci and have been reported to be preceded by the competence regulation locus . In encodes a serine protease. In plays a role in sporulation and partition. encodes an initiator replication protein, very well conserved in several bacteria and encodes the β subunit of DNA polymerase III in . The function of is unknown. The N-terminal part of another reading frame, , revealed high homology with a GTP-binding protein. DnaA box clusters were found upstream and downstream from . The presence of two such clusters suggests that the chromosomal origin of is located within this region. The position of , and therefore the putative origin of replication, were localized on the physical map of .

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1998-02-01
2021-07-25
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