1887

Abstract

Summary: Cryopreparation of live sporozoites and oocysts of the apicomplexan parasite , followed by transmission electron microscopy, was undertaken to show the 3D arrangement of organelles, their number and distribution. Profiles of parasites obtained from energy-filtering transmission electron microscopy of serial sections provided 3D reconstructions from which morphometric data and stereo images were derived. The results suggest that sporozoites have a single rhoptry containing an organized lamellar body, no mitochondria or conventional Golgi apparatus, and one or two crystalline bodies. Micronemes were shown to be spherical, numerous and apically located, and to account for 0·8% of the total cell volume. Dense granules were less numerous, larger, accounted for 5·8% of the cell volume, and were located more posteriorly than micronemes. A structure juxtaposed to the nucleus with similarities to the plastid-like organelle reported for other members of the Apicomplexa was observed. The detailed analysis illustrates the advantages of cryopreparation in retaining ultrastructural fidelity of labile or difficult to preserve structures such as the sporozoite of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-144-12-3343
1998-12-01
2021-04-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/144/12/mic-144-12-3343.html?itemId=/content/journal/micro/10.1099/00221287-144-12-3343&mimeType=html&fmt=ahah

References

  1. Bonnet P., Lacourt I., Venard P., Ricci P. 1994; Diversity in pathogenicity and in elicitin production among isolates of Pbytophtbora parasitica.. J Phytopathol 141:25–37
    [Google Scholar]
  2. Bonnet P., Bourdon E., Ponchet M., Blein J.-P., Ricci P. 1996; Acquired resistance triggered by elicitins in tobacco and other plants.. Eur J Plant Pathol 102:181–192
    [Google Scholar]
  3. Campbell A.M., Moon R.P., Duncan J.M., Gurr S.-J., Kinghorn J.R. 1989; Protoplast formation and regeneration from sporangia and encysted zoospores of Phytophthora infestans.. Physiol Mol Plant Pathol 34:299–307
    [Google Scholar]
  4. Elliott C.G., Hendrie M.R., Knights B.A. 1966; The sterol requirement of Phytophthora cactorum.. J Gen Microbiol 42:425–435
    [Google Scholar]
  5. Erwin D.C., Ribeiro O.K. 1996 Phytophthora Diseases Worldwide. St Paul, MN: American Phytopathological Society;
    [Google Scholar]
  6. Judelson H.S. 1993; Efficient co-transformation mediated by intermolecular ligation occurring in vivo in the oomycete Phytophthora infestans.. Mol Gen Genet 239:241–250
    [Google Scholar]
  7. Judelson H.S. 1996; Recent advances in the genetics of oomycete plant pathogens.. Mol Plant-Microbe Interact 7:302–304
    [Google Scholar]
  8. Judelson H.S., Whittaker S.L. 1995; Inactivation of transgenes in Phytophthora infestans is not associated with their deletion, methylation, or mutation.. Curr Genet 28:571–579
    [Google Scholar]
  9. Judelson H.S., Tyler B.M., Michelmore R.W. 1991; Stable transformation of the oomycete pathogen Phytophthora infestans.. Mol Plant-Microbe Interact 4:602–607
    [Google Scholar]
  10. Judelson H.S., Dudler R., Pieterse C.M.J., Unkles S.E. 1993; Expression and antisense inhibition of transgenes in Phytophthora infestans is modulated by choice of promoter and position effects.. Gene 133:63–69
    [Google Scholar]
  11. Kamoun S., Young M., Glascock C.B., Tyler B.M. 1993; Extracellular protein elicitors from Phytohpthora: host-specificity and induction of resistance to bacterial and fungal phytopathogens.. Mol Plant-Microbe Interact 6:15–22
    [Google Scholar]
  12. Kamoun S., van West P., de Jong A.J., de Groot K.E., Vieeshouwers V.G.A.A., Govers F. 1997; A gene encoding a protein elicitor of Phytophthora infestans is down-regulated during infection of potato.. Mol Plant-Microbe Interact 10:13–20
    [Google Scholar]
  13. Keller H., Bonnet P., Galiana E., Pruvot L., Friedrich L., Ryals J., Ricci P. 1996; Salicylic acid mediates elicitin-induced sytemic acquired resistance, but not necrosis in tobacco.. Mol Plant-Microbe Interact 9:696–703
    [Google Scholar]
  14. Le Berre J.-Y., Panabières F., Ponchet M., Deneroy L., Bonnet P., Marais A., Ricci P. 1994; Occurrence of multiple forms of elicitins in Phytophthora cryptogea.. Plant Physiol Biochem 32:251–258
    [Google Scholar]
  15. Moon R.P., Unkles S.E., Duncan J.M., Hawkins A.R., Kinghorn J.R. 1992; Sequence of the Phytophthora infestans glyceraldehyde-3-phosphate dehydrogenase-encoding genes (gpdA).. Plant Mol Biol 18:1209–1211
    [Google Scholar]
  16. Panabières F., Marais A., Le Berre J.-Y., Penot I., Fournier D., Ricci P. 1995; Characterisation of a gene cluster of Phytophthora cryptogea which codes for elicitins, proteins inducing a hypersensitive-like response in tobacco.. Mol Plant-Microbe Interact 8:996–1003
    [Google Scholar]
  17. Panabières res, Ponchet M., Allasia V., Cardin L., Ricci P. 1997; Characterisation of border species among Pythiaceae: several Pythiums produce elicitins, typical proteins from Phytophthora spp.. Mycol Res 101:1459–1468
    [Google Scholar]
  18. Penot I. 1996 Induction de necrose et de resistance chez le tabac par une famille de proteines fongiques; les elicitines. These de doctorat, Université Claude Bernard
    [Google Scholar]
  19. Raeder U., Broda P. 1985; Rapid preparation of DNA from filamentous fungi.. Lett Appl Microbiol 1:17–20
    [Google Scholar]
  20. Ricci P. 1997; Induction of the hypersensitive response and systemic acquired resistance by fungal proteins: the case of elicitins.. In Plant-Microbe Interactions 3 pp. 53–75 Edited by Stacey G., Keen N.T. New York: Chapman & Hall;
    [Google Scholar]
  21. Ricci P., Bonnet P., Huet J.C., Sallantin M., Beauvais-Cante F., Bruneteau M., Billard V., Michel G., Pernollet J.C. 1989; Structure and activity of proteins from pathogenic fungi Phytophthora eliciting necrosis and acquired resistance in tobacco.. Eur J Biochem 183:555–563
    [Google Scholar]
  22. Ricci P., Trentin F., Bonnet P., Venard P., Mouton-Perronnet F., Bruneteau M. 1992; Differential production of parasiticein, an elicitor of necrosis and resistance in tobacco, by isolates of Phytophthora parasitica.. Plant Pathol 41:298–307
    [Google Scholar]
  23. Ricci P., Panabières res, Bonnet P. 7 other authors 1993; Proteinaceous elicitors of plant defense responses.. In Mechanisms of Plant Defense Responses pp. 121–135 Edited by Legrand M., Fritig B. Dordrecht: Kluwer;
    [Google Scholar]
  24. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Yu L.M. 1995; Elicitins from Phytophthora and basic resistance in tobacco.. Proc Natl Acad Sci USA 924088–4094
    [Google Scholar]
  26. Yu L.M. 1997; The isolation and assay of elicitins.. In Methods in Plant Biochemistry and Molecular Biology pp. 265–279 Edited by Dashek W. V. New York: CRC Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-144-12-3343
Loading
/content/journal/micro/10.1099/00221287-144-12-3343
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error