1887

Abstract

Summary: Using enrichment methods, a new bacteriophage (M1) was isolated, which is capable of generalized transduction in subsp. () strain SCRI1043. M1 is probably a virulent phage and contains double-stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2 were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. M1 infected over 25% of strains tested and so may be useful both for the genetic analysis of a number of isolates and for the transductional transfer of selectable markers between strains.

Funding
This study was supported by the:
  • NERC, UK (Award GR3/8003)
  • BBSRC, UK (Award PO3960)
  • Scottish Office Agriculture, Environment and Fisheries Department
  • BBSRC/CASE student with SCRI
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/content/journal/micro/10.1099/00221287-143-7-2433
1997-07-01
2021-05-15
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