Summary: The genes encoding pyruvate dehydrogenase (PDH) of were previously located by cloning and sequence analysis of the region upstream of the genes encoding the citrate synthase and -glutamylcysteine synthetase genes. The genes of were able to complement an mutant for growth on minimal medium lacking acetate, indicating that the PDH complex was functional in The predicted amino acid sequence of the PDH complex contained three ORFs. The first ORF encoded a 36.7 kDa homologue of the PDH complex E1α subunit, the second ORF a 37.4 kDa E1α subunit and the third ORF an unusual 102 kDa fusion of the E2 and E3 subunits. In spite of being a Gram-negative bacterium, its PDH complex had more features in common with Gram-positive bacteria and eukaryotes.


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