1887

Abstract

The specific interaction between rhizobia and their hosts requires many genes that influence both early and late steps in symbiosis. Three new genes, designated (protein secretion) and were identified adjacent to the mutation in a cosmid carrying the genomic DNA of bv. TA1. The genes share significant homology to the genes encoding ABC transporter proteins PrtDE from and AprDE from which export the proteases in these bacteria. PrsD shows at least five potential transmembrane hydrophobic regions and a large hydrophilic domain containing an ATP/GTP binding cassette. has only one potential transmembrane hydrophobic domain in the N-terminal part and is proposed to function as an accessory factor in the transport system. ORF3, like PrtF and AprF, has a typical N-terminal signal sequence but has no homology to these proteins. The insertion of a kanamycin resistance cassette into the gene of the bv. TA1 wild-type strain created a mutant which produced a normal amount of exopolysaccharide but was not effective in the nodulation of clover plants.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-143-4-1389
1997-04-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/143/4/mic-143-4-1389.html?itemId=/content/journal/micro/10.1099/00221287-143-4-1389&mimeType=html&fmt=ahah

References

  1. Akatsuka H., Kawai E., Omori K., Shibatani T. 1995; The three genes lipB, lipC, and lipD involved in the extracellular secretion of the Serratia marcescens lipase which lacks an Nterminal signal peptide.. J Bacteriol 177:6311–6389.
    [Google Scholar]
  2. Becker A., Küster H., Niehaus K., Pühler A. 1995; Extension of the Rhizobium meliloti succinoglycan biosynthesis gene cluster: identification of the exsA gene encoding an ABC transporter protein, and the exsB gene which probably codes for a regulator of succinoglycan biosynthesis.. Mol Gen Genet 249:487–497.
    [Google Scholar]
  3. Bialek U., Skorupska A., Yang W.-C., Bisseling T., Van Lammeren A. A. M. 1995; Disturbed gene expression and bacterial development in Trifolium pratense root nodules induced by a Tn5 mutant in exopolysaccharide synthesis.. Planta 197:184–192.
    [Google Scholar]
  4. Chen H., Batley M., Redmond J., Rolfe B. G. 1985; Alteration of the effective nodulation properties of a fast-growing broad host range Rhizobium due to changes in exopolysaccharide synthesis.. J Plant Physio 120:331–349.
    [Google Scholar]
  5. Delepelaire P., Wandersman C., Redmond J. 1991; Characterization, localization and transmembrane organization of the three proteins PrtD, PrtE and PrtF necessary for protease secretion by the Gram-negative bacterium Erwinia chrysanthemi.. Mol Microbiol 5:2427–2434.
    [Google Scholar]
  6. Derylo M., Skorupska A., Bednara J., Lorkiewicz Z. 1986; Rhizobium trifolii mutants deficient in exopolysaccharide production.. Physiol Plant 66:699–704.
    [Google Scholar]
  7. Ditta G., Stanfield S., Corbin D., Helinski D. R. 1980; Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.. Proc Natl Acad Sci USA 77:7347–7351.
    [Google Scholar]
  8. Djordjevic M. A., Schofield P. R., Rolfe B. G. 1985; Tn5 mutagenesis of Rhizobium trifolii host-specific nodulation genes results in mutants with altered host-range ability.. Mol Gen Genet 200:463–471.
    [Google Scholar]
  9. Duong F., Lazdunski A., Cami B., Murgier M. 1992; Sequence of cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.. Gene 121:47–54.
    [Google Scholar]
  10. Eisenberg D., Schwarz E., Komaramy M., Wall R. 1984; Analysis of membrane and surface protein sequences with the hydrophobic moment plot.. J Mol Biol 179:125–142.
    [Google Scholar]
  11. Fath M. J., Kolter R., Komaramy M. 1993; ABC transporters: bacterial exporters.. Microbiol Rev 57:995–1017.
    [Google Scholar]
  12. Guzzo J., Duong F., Wandersman C., Murgier M., Lazdunski A. 1991; The secretion genes of Pseudomonas aeruginosa alkaline protease are functionally related to those of Erwinia chrysanthemi proteases and Escherichia coli α-haemolysin.. Mol Microbiol 5:447–453.
    [Google Scholar]
  13. Hardy R. W., Holsten R. D., Jackson E. K., Burns C. 1982; The C2H2-C2H4 assay for N2 fixation. Laboratory and field evolution.. Plant Physiol 43:1185–1210.
    [Google Scholar]
  14. Higgins C. F. 1992; ABC transporters: from microorganisms to man.. Annu Rev Cell Bio 8:67–113.
    [Google Scholar]
  15. Jimenez-Zurdo J. I., Mateos P. F., Dazzo F., Martinez-Molina E. 1996; Influence of the symbiotic plasmid (pSym) on cellulase production by Rhizobium leguminosarum bv. trifolii ANU843.. Soil Biol Biochem 28:131–133.
    [Google Scholar]
  16. Leigh J. A., Walker G. 1994; Exopolysaccharides of Rhizobium: synthesis, regulation and symbiotic function.. Trends Genet 10:63–67.
    [Google Scholar]
  17. Letoffé S., Delepelaire P., Wandersman C. 1990; Protease secretion by Erwinia chrysmthemi: the specific secretion functions are analogous to those of Escherichia coli α-haemolysin.. EMBO J 9:1375–1382.
    [Google Scholar]
  18. Letoffé S., Ghigo J. M., Wandersman C. 1993; Identification of two components of Serratia marcescens metalloprotease transporter: protease SM secretion in Escherichia coli is TolC dependent.. J Bacteriol 175:7321–7328.
    [Google Scholar]
  19. Mateos P. F., Jimenez-Zurdo J. I., Chen J., Squartini A., Haack S. K., Martinez-Molina E., Hubbell D. H., Dazzo F. 1992; Cellassociated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii.. Appl Environ Microbiol 58:1816–1822.
    [Google Scholar]
  20. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison.. Proc Natl Acad Sci USA 85:2444–2448.
    [Google Scholar]
  21. Reuber T. L., Walker G. C. 1993; Biosynthesis of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti.. Cell 74:269–280.
    [Google Scholar]
  22. Ruvkun G. B., Ausubel F. M. 1981; A general method for site directed mutagenesis in prokaryotes.. Nature 289:85–90.
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY:. Cold Spring Harbor Laboratory.
    [Google Scholar]
  24. Scheu A. K., Economou A., Hong G. F., Ghelani S., Johnston A. W. B., Downie J. A. 1992; Secretion of the Rhizobium leguminosarum nodulation protein NodO by haemolysin type systems.. Mol Microbiol 6:231–238
    [Google Scholar]
  25. Schofield P. R., Watson J. M. 1986; DNA sequence of Rhizobiurn trifolii nodulation genes reveals a reiterated and potentially regulatory sequence preceding nodABC and nodEF.. Nucleic Acids Res 14:2891–2903.
    [Google Scholar]
  26. Schuler G. D., Altschu S. F., Lipman D. J. 1991; A workbench for multiple alignment construction and analysis.. Protein Struct Funct Genet 9:180–190.
    [Google Scholar]
  27. Schulze M., Kondoros E. F., Ratet P., Buire M., Kondoros A. 1994; Cell and molecular biology of Rhizobium-plant interactions.. Intern Rev Cytol 156:1–75.
    [Google Scholar]
  28. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria.. Bio/Technology 1:784–791.
    [Google Scholar]
  29. Skorupska A., Derylo M., Golinowski W. 1991; The region for exopolysaccharide synthesis on Rhizobium leguminosarum bv. trifolii is located on the non-symbiotic plasmid.. Acta Biochim Polon 38:423–435.
    [Google Scholar]
  30. Skorupska A., Bialek U., Urbanik-Sypniewska T., Van Lammeren A. 1995; Two types of nodules induced on Trifolium pratense by mutants of Rhizobium leguminosarum bv. trifolii deficient in exopolysaccharide production.. J Plant Physiol 147:93–100.
    [Google Scholar]
  31. Stanfield S. W., lelpi L., O’Brochta, D., Helinski R. D., Ditta G. S. 1988; The ndvA gene product of Rhizobium meliloti is required for β-(l-2)glucan production and has homology to the ATP-binding export protein HlyB.. J Bacteriol 170:3523–3530.
    [Google Scholar]
  32. Stanley J., Dowling D. N., Stucker M., Broughton W. J. 1987; Screening costramid libraries for chromosomal genes: an alternative interspecific hybridization method.. FEMS Microbiol Lett 48:25–30.
    [Google Scholar]
  33. Sutton M., Lea E. J., Downie J. A. 1994; The nodulationsignaling protein NodO from Rhizobium leguminosarum biovar viciae forms ion channels in membranes.. Proc Nut1 Acad Sci USA 91:9990–9994.
    [Google Scholar]
  34. Walker J. E., Sarste M., Runswick M. J., Gay N. J. 1982; Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.. EMBO J 1:945–951.
    [Google Scholar]
  35. Wandersman C., Letoffé S. 1993; Involvement of lipopolysaccharide in the secretion of Escherichia coli α-haemolysin and Erwinia chrysanthemi proteases.. Mol Microbiol 7:141–150.
    [Google Scholar]
/content/journal/micro/10.1099/00221287-143-4-1389
Loading
/content/journal/micro/10.1099/00221287-143-4-1389
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error