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Volume 143, Issue 3

Analysis of a gene encoding a protein homologous to glutathione -transferases Free

Abstract

A novel gene, , the sequence of which indicated that it was a member of the gene family of glutathione -transferases (GSTs), was identified. The homology was greatest to the GST enzymes of higher plants. The gene was normally expressed at a very low level. The product of was over-expressed and purified from . It was shown to bind to the affinity matrix glutathione-Sepharose, but no enzymic GST activity with 1-chloro-2,4-dinitrobenzene as substrate was detected. encoded a polypeptide of 203 amino acid residues with a calculated molecular mass of 21990 Da. Transcribed divergently from is another gene, , which was similar in sequence to the LysR family of bacterial transcriptional regulators. A mutation in had no effect on the transcription of itself or under the growth conditions used here. Mutations in and caused no obvious phenotypic defect and the biological functions of these genes remain to be determined.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): glutathione , glutathione S-transferase , Rhizobium and transcriptional regulator
© Society for General Microbiology 1997
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1997-03-01
2024-04-27
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Analysis of a Rhizobium leguminosarum gene encoding a protein homologous to glutathione S-transferases
Microbiology 143, 813 (1997); https://doi.org/10.1099/00221287-143-3-813
/content/journal/micro/10.1099/00221287-143-3-813
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