1887

Abstract

The O-antigenic specificities found among salmonellae of serogroup C are O:67, O:67, O:667 and O:6,7,14, as defined by classical serology. Factor O:7 is the group-wide determinant while factors O:6 O:6 and O:14 are found in some strains but not others. Strains of the O:67 specificity are subject to lysogenic conversion by phages 6 and 14 to the O:67 and O:6,7,14 specificities, respectively. To further delineate antigenic complexity and serological relationships among strains of this serogroup monoclonal antibodies (mAbs) were generated against the O:667 polysaccharide of Five mAbs of either the O:6 or the O:6 specificities did not bind O:6,7,14 strains or LPS, showing that the O:6 determinant in these strains is neither O:6 nor O:6. Thus antigenic conversion of O:67 strains by phage 14 is accompanied by addition of O:14 as well as loss of O:6 Three mAbs which demonstrated group-wide reactivity, and were thus specific for O:7, recognized clearly separable epitopes hereby defined as sub-specificities, O:7 O:7 and O:7 Immunoblotting of mAbs against electrophoretically resolved LPS showed that factors O:6 and O:6 are expressed only in LPS molecules of high molecular mass whereas O:7 and O:7 are expressed only in relatively low-molecular-mass chains. These results are consistent with the expression of different antigenic determinants in structurally distinct subpopulations of O chains. The implication of the existence of distinct subpopulation of chains is that the published structure of the O:6,7 repeat unit is not fully representative of the O-antigenic structure of this group.

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1997-02-01
2021-10-19
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References

  1. Beutler B., Cerami A. 1988; Tumor necrosis, cachexia, shock and inflammation: a common mediator.. Annu Rev Biochem 57:505–518
    [Google Scholar]
  2. Carlin N.I.A., Lindberg A.A. 1983; Monoclonal antibodies specific for O-antigenic polysaccharides of Shigella flexneri: clones binding to II, II: 3,4, and 7,8 epitopes.. J Clin Microbiol 18:1183–1189
    [Google Scholar]
  3. Di-Padova F.E., Mikol V., Barclay G.R., Poxton I.R., Brade H., Rietschel E.T. 1994; Anti-lipopolysaccharide core antibodies.. In Bacterial Endotoxins: Basic Science to Anti-sepsis Strategies pp. 85–94 Edited by Levin J., van Deventer S. J. H., van der Poll T., Sturk A. New York: Wiley-Liss;
    [Google Scholar]
  4. Edwards P.R. 1945; Form variation in group C Salmonella strains.. Proc Soc Exp Biol Med 5949–52
    [Google Scholar]
  5. Ekwall E., Svenson S.B., Lindberg A.A. 1982; Identification of Salmonella of serogroup C1 by immunofluorescence and coagglutination with antiserum against an oligosaccharide-protein conjugate.. J Med Microbiol 15:173–180
    [Google Scholar]
  6. Elkins K., Metcalf E.S. 1984; Monoclonal antibodies demonstrate multiple epitopes on the O antigens of Salmonella typhimurium LPS.. J Immunol 133:2255–2260
    [Google Scholar]
  7. Escobar M.R., Edwards P.R. 1968; Lysogenic conversion of O antigens in Salmonella serogroup C1.. Can J Microbiol 14:453–458
    [Google Scholar]
  8. Finlay B.B., Starnbach M.N., Francis C.L., Stocker B.A., Chatfield S., Dougan G., Falkow S. 1988; Identification and characterization of TnphoA mutants of Salmonella that are unable to pass through a polarised MDCK epithelial cell monolayer.. Mol Microbiol 2:757–763
    [Google Scholar]
  9. Fuller N.A., Staub A.M. 1968; Immunochemical studies on Salmonella. 13. Chemical changes appearing on the specific polysaccharide of S. choleraesuis (62,7) after its conversion by phage 14 (6,7).. Eur J Biochem 4:286–300
    [Google Scholar]
  10. Fuller N.A., Etievant M., Staub A.-M. 1968; Immunochemical studies on Salmonella. 14. Oligosaccharides which determine the specificities 62,7 and 14 in S. choleraesuis, after conversion by bacteriophage 14 (6,7).. Eur J Biochem 6:525–533
    [Google Scholar]
  11. Galfre G., Milstein C., Wright B. 1979; Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.. Nature 277:131–133
    [Google Scholar]
  12. Helander I.M., Moran A.P., Mäkelä P.H. 1992; Separation of lipopolysaccharide populations with different contents of O- antigen factor 122 in Salmonella enterica serovar Typhimurium.. Mol Microbiol 6:2857–2862
    [Google Scholar]
  13. Hitchcock P.J., Brown T.M. 1983; Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.. J Bacteriol 154:269–277
    [Google Scholar]
  14. Jörbeck H.J.A., Svenson S.B., Lindberg A.A. 1979; Immuno-chemistry of Salmonella O-antigens: specificity of rabbit antibodies against the O-antigen 4 determinant elicited by whole bacteria and O antigen 4-specific saccharide-protein conjugates.. J Immunol 123:1376–1381
    [Google Scholar]
  15. Kalisiak A., Oosterwijk E., Minniti J.G., Old L.J., Scheinberg D.A. 1991; A monoclonal antibody for terminal β-galactose: use in analysis of glycosphingolipids.. Glycoconj J 3:311–319
    [Google Scholar]
  16. Kauffmann F. 1966 The Bacteriology of Enterobacteriaceae. Copenhagen: Munksgaard;
    [Google Scholar]
  17. Lam M.Y.C., McGroarty E.J., Kropinski A.M., MacDonald L.A., Pedersen S.S., Hoiby N., Lam J.S. 1989; Occurrence of a common lipopolysaccharide antigen in standard and clinical strains of Pseudomonas aeruginosa.. J Clin Microbiol 27:962–967
    [Google Scholar]
  18. LeMinor L. 1965; Conversions antigéniques chez les Salmonella. VII. Acquisition du facteur 14 par les Salmonella su sous-groupe C: (6,7), après lysogénisation par une phage tempéré isolé de cultures du sous-groupe C1 [6, (7), (14) (*)].. Ann Inst Pasteur 109:505–515
    [Google Scholar]
  19. LeMinor L. 1968; Conversions antigéniques chez les Salmonella. X. Etude d’un nouveau phage convertisseur modifiant la structure antigenique O des souches du groupe C1 . Ann Inst Pasteur 114:49–62
    [Google Scholar]
  20. LeMinor L., Popoff M.Y. 1988 Antigenic Formulas of the Salmonella Serovars 5th revision Paris: WHO Collaborating Center for Reference and Research on Salmonella, Institut Pasteur;
    [Google Scholar]
  21. LeMinor L., Rhode R. 1989 Guidelines for the Preparation of Salmonella Antisera. Paris: WHO Collaborating Center for Reference and Research on Salmonella, Institut Pasteur.;
    [Google Scholar]
  22. Liang-Takasaki C.J., Mäkelä P.H., Leive L. 1982; Phagocytosis of bacteria by macrophages: changing the carbohydrate of lipopolysaccharide alters interaction with complement and macrophages.. J Immunol 128:1229–1235
    [Google Scholar]
  23. Liang-Takasaki C.J., Saxen H., Mäkelä P.H., Leive L. 1983; Complement activation by polysaccharide of LPS: an important virulence determinant for salmonellae.. Infect Immun 41:563–569
    [Google Scholar]
  24. Lightfoot J., Lam J.S. 1991; Molecular cloning of genes involved with expression of A-band lipopolysaccharide, an antigenically conserved form, in Pseudomonas aeruginosa.. J Bacteriol 173:5624–5630
    [Google Scholar]
  25. Lind S. 1992 PhD thesis, Karolinska Institute, Stockholm, Sweden.
  26. Lindberg A.A., Le Minor L. 1984; Serology of Salmonella.. Methods Microbiol 15:1–141
    [Google Scholar]
  27. Lindberg B., Leontein K., Lindqvist U., Svensson S.B., Wrangsell G., Dell A., Rogers M. 1988; Structural studies of the O-antigenic polysaccharide of Salmonella thompson, sero-group C1 (6,7).. Carbohydr Res 174:313–322
    [Google Scholar]
  28. Luk J.M.C., Lindberg A.A. 1991; Anti-Salmonella lipopoly- saaccharide monoclonal antibodies: characterization of Salmonella BO-, CO-, DO-, and EO-specific clones and their diagnostic usefulness.. J Clin Microbiol 29:2424–2433
    [Google Scholar]
  29. Luk J.M.G, Nnalue N. A., Lindberg A.A. 1990; Efficient production of mouse and rat monoclonal antibodies against Salmonella serogroup Cl using LPS-coated bacteria as immunogen.. J Immunol Methods 129:243–250
    [Google Scholar]
  30. Mäkelä P.H., Stocker B.A.D. 1984; Genetics of lipopoly-saccharide.. In Handbook of Endotoxin, vol. 1, Chemistry of Endotoxin pp. 59–137 Edited by Rietschel E.T. Amsterdam: Elsevier;
    [Google Scholar]
  31. Mintz GS., Deibel R.H. 1983; Effect of lipopolysaccharide mutations on the pathogenesis of experimental Salmonella gastroenteritis.. Infect Immun 40:236–244
    [Google Scholar]
  32. Nnalue N.A., Lindberg A.A. 1990; Salmonella choleraesuis strains deficient in O antigen remain fully virulent for mice by parenteral inoculation but are avirulent by oral administration.. Infect Immun 58:2493–2501
    [Google Scholar]
  33. Nnalue N.A., Newton S., Stocker B.A.D. 1990; Lysogenisation of Salmonella choleraesuis by phage 14 increases average length of O-antigen chains, serum resistance and intraperitoneal mouse virulence.. Microb Pathog 8:393–402
    [Google Scholar]
  34. Nnalue N.A., Weintraub A., Lindberg A.A. 1991; Properties of a rat monoclonal antibody reactive with both the mannan of Candida species and the O-antigen 6,7 polysaccharide of serogroup C1 salmonellae.. Infect Immun 59:229–233
    [Google Scholar]
  35. Nnalue N.A., Lind S., Lindberg A.A. 1992; The disaccharide L-α-d-heptosel → 7-L-α-d-heptosel → of the inner core domain of Salmonella LPS is accessible to antibody and is the epitope of a broadly reactive monoclonal antibody.. J Immunol 149:2722–2728
    [Google Scholar]
  36. Nnalue N.A., Weintraub A., Oscarson S., Lindberg A.A. 1994; Cross-reactivity between the mannan of Candida species, Klebsiella K24 polysaccharide and Salmonella C1 and E O- antigens is mediated by a terminal non-reducing β-mannosyl residue.. Eur J Biochem 220:973–979
    [Google Scholar]
  37. Ørskov I., Ørskov F., Jann B., Jann K. 1977; Serology, chemistry and genetics of O and K antigens of Escherichia coli.. Bacteriol Rev 41:667–710
    [Google Scholar]
  38. Rivera M., Bryan L.E., Hancock R.E.W., McGroarty E.J. 1988; Heterogeneity of lipopolysaccharides from Pseudomonas aeruginosa: analysis of lipopolysaccharide chain length.. J Bacteriol 170:512–521
    [Google Scholar]
  39. Slauch J.M., Mahan M.J., Michetti P., Neutra M.R., Mekelanos J.J. 1995; Acetylation (O-factor 5) affects the structural and immunological properties of Salmonella typhimurium lipopolysaccharide O antigen.. Infect Immun 63:437–441
    [Google Scholar]
  40. Smith H.W., Parsell Z. 1974; The effect on virulence of converting the O antigen of Salmonella choleraesuis from 62,7 to 61,7 by phage.. J Gen Microbiol 81:217–224
    [Google Scholar]
  41. Tsai C.M., Frasch GE. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.. Anal Biochem 119:115–119
    [Google Scholar]
  42. Valtonen V.V. 1970; Mouse virulence of Salmonella strains: the effect of different smooth-type O-side chains.. J Gen Microbiol 69:107–115
    [Google Scholar]
  43. Valtonen V.V., Mäkelä P.H. 1971; The effect of lipopolysaccharide modifications - antigenic factors 1,5,122 and 27-on the virulence of Salmonella strains for mice.. J Gen Microbiol 69:107–115
    [Google Scholar]
  44. Weintraub A., Lindberg A.A., Lipniunas P., Nilsson B. 1988; Heterogeneity in oligosaccharides from the O-polysaccharide chains of the lipopolysaccharide from Salmonella typhi 253Ty determined by Fast Atom Bombardment Mass Spectrometry.. Glycoconj J 5:207–213
    [Google Scholar]
  45. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure.. Methods Carbohydr Chem 5:80–91
    [Google Scholar]
  46. Whitfield G, Richards J. C., Perry M. B., Clarke B.R., MacLean L.L. 1991; Expression of two structurally distinct d-galactan O antigens in the lipopolysaccharide of Klebsiella pneumoniae serotype Ol.. J Bacteriol 173:1420–1431
    [Google Scholar]
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