1887

Abstract

The gene from the dimorphic fungus was cloned by functional complementation of the arginine auxotrophy present in strain EL2 (Arg) using a gene library constructed in the double autonomously replicating sequence vector pRM1. Sequence analysis revealed a putative 857 amino acid polypeptide (95 kDa) which showed high homology (63% protein identity) to the 6 gene. Similarly to the gene, the gene is responsible for both the acetylglutamate kinase and acetylglutamyl-phosphate reductase activities, the second and third steps of arginine biosynthesis at the mitochondria. The gene complemented the mutation present in (strain D160-4D) on a yeast episomal plasmid using its own regulatory signals. A set of non-integrative high-efficiency plasmid vectors based on this gene marker was constructed and a null ,6d strain was obtained using the common -blaster strategy. In addition, we generated an ,6d null mutant in a single transformation event, thus improving the basic strategy for generating gene deletions in

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1997-02-01
2021-05-12
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References

  1. Boonchird C., Messenguy F., Dubois E. 1991; Charac-terization of the yeast ARG5,6 gene: determination of the nucleotide sequence, analysis of the control region and of ARG5,6 transcript.. Mol Gen Genet 226:154–166
    [Google Scholar]
  2. Cannon R.D., Jenkinson H.F., Shepherd M.G. 1990; Isolation and nucleotide sequence of an autonomously replicating sequence (ARS) element functional in Candida albicans and Saccharomyces cerevisiae.. Mol Gen Genet 221:210–218
    [Google Scholar]
  3. Cannon R.D., Jenkinson H.F., Shepherd M.G. 1992; Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in C.. albicans and in Saccharomyces cerevisiae. Mol Gen Genet 235:453–457
    [Google Scholar]
  4. Fonzi W.A., Irwin M.Y. 1993; Isogenic strain construction and gene mapping in Candida albicans.. Genetics 134:717–728
    [Google Scholar]
  5. Gil C., Pomés R., Nombela C. 1988; A complementation analysis by parasexual recombination of Candida albicans morphological mutants.. J Gen Microbiol 134:1587–1595
    [Google Scholar]
  6. Gil C., Pomés R., Nombela C. 1990; Isolation and charac-terization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures.. J Bacteriol 172:2384–2391
    [Google Scholar]
  7. Gillum A.M., Tsay E.Y.H., Kirsch D.R. 1984; Isolation of theCandida albicans gene for orotidine-5/-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations.. Mol Gen Genet 198:179–182
    [Google Scholar]
  8. Gorman J.A., Chan W., Gorman J.W. 1991; Repeated use of GAL1 for gene disruption in Candida albicans.. Genetics 129:19–24
    [Google Scholar]
  9. Goshorn A.K., Scherer S. 1989; Genetic analysis of proto-trophic natural variants of Candida albicans.. Genetics 123:667–673
    [Google Scholar]
  10. Goshorn A.K., Grindle S.M., Scherer S. 1992; Gene isolation by complementation in Candida albicans and applications to physical and genetic mapping.. Infect Immun 60:876–884
    [Google Scholar]
  11. Hanahan D. 1988; Techniques for transformation of E. coli. . In DNA Cloning pp. 109–135 Glover D.M., Bhattacharya SK. Edited by Oxford: IRL Press;
    [Google Scholar]
  12. Herreros E., García-Sáez M.I., Nombela C., Sánchez M. 1992; A reorganized Candida albicans DNA sequence promoting homologous non-integrative genetic transformation.. Mol Microbiol 6:3567–3574
    [Google Scholar]
  13. Hill J.E., Myers A.M., Koerner T.J., Tzagoloff A. 1986; Yeast/E. coli shuttle vectors with multiple unique restriction sites.. Yeast 2:163–167
    [Google Scholar]
  14. Hoyer L.L., Magee B.B., Rikkerink E.H., Scherer S. 1994; The ARG4 gene of Candida albicans.. Gene 2:213–218
    [Google Scholar]
  15. van Huffel C., Dubois E., Messenguy F. 1992; Cloning and sequencing of arg3 and argl1 genes of Schizosacchavomyces pombe on a 10-kb DNA fragment. Heterologous expression and mitochondria1 targeting of their translation products.. Eur J Biochem 205:33–43
    [Google Scholar]
  16. Kelly R., Miller S.M., Kurtz M.B., Kirsch D.R. 1987; Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants.. Mol Cell Biol 7:199–208
    [Google Scholar]
  17. Kurtz M.B., Cortelyou M.W., Kirsch D.R. 1986; Integrative transformation of Candida albicans using a cloned Candida ADE2 gene.. Mol Cell Biol 6:142–149
    [Google Scholar]
  18. Kurtz M.B., Cortelyou M.W., Miller S.M., Lai M., Kirsch D.R. 1987; Development of autonomously replicating plasmids for Candida albicans.. Mol Cell Biol 7:209–217
    [Google Scholar]
  19. Kurtz M.B., Kelly R., Kirsch D.R. 1990; Molecular genetics of Candida albicans. . In The Genetics of Candida pp. 21–74 Kirsch D.R., Kelly R., Kurtz M.B. Edited by Boca Raton: CRC Press;
    [Google Scholar]
  20. Navarro-García F., Sánchez M„Pla, Pla J., Nombela C. 1995; Functional characterization of the MKC1 gene of Candida albicans, which encodes a mitogen-activated protein kinase homolog related to cell integrity.. Mol Cell Biol 4:2197–2206
    [Google Scholar]
  21. Odds F.C. 1988 Candida and Candidosis, 2nd edn.. London: Bailliere Tindall;
    [Google Scholar]
  22. Pearson W.R., Lipman D.J. 1988; Improved tools for biological sequence comparison.. Proc Natl Acad Sci USA 85:2444–2448
    [Google Scholar]
  23. Pereira S.A., Livi G.P. 1995; A GCN-like response in Candida albicans.. Cell Biol lnt 19:65–69
    [Google Scholar]
  24. Pla J., Pérez-Díaz R.M., Navarro-García F., Sánchez M., Nombela C. 1995; Cloning of Candida albicans H1S1 gene by direct homologous complementation of a histidine auxotroph using an improved double-ARS shuttle vector.. Gene 165:115–120
    [Google Scholar]
  25. Rosenbluh A., Mevarech M., Koltin Y., Gorman J.A. 1985; Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae.. Mol Gen Genet 200:500–502
    [Google Scholar]
  26. Sadhu G, McEachern M.J., Reed S.I., Hicks J.B. 1992; A G-protein alpha subunit from asexual Candida albicans functions in the mating signal transduction pathway of Saccharomyces cerevisiae and is regulated by the al-alpha 2 repressor.. Mol Cell Biol 12:1977–1985
    [Google Scholar]
  27. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencingwith chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  29. Scherer S., Magee P.T. 1990; Genetics of Candida albicans.. Microbiol Rev 54:226–241
    [Google Scholar]
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